Quantifying the inhibitory impact of soluble phenolics on anaerobic carbon mineralization in a thawing permafrost peatland
The mechanisms controlling the extraordinarily slow carbon (C) mineralization rates characteristic of Sphagnum-rich peatlands (“bogs”) are not fully understood, despite decades of research on this topic. Soluble phenolic compounds have been invoked as potentially significant contributors to bog peat...
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ftpubmed:oai:pubmedcentral.nih.gov:8809605 2023-05-15T17:58:01+02:00 Quantifying the inhibitory impact of soluble phenolics on anaerobic carbon mineralization in a thawing permafrost peatland Cory, Alexandra B. Chanton, Jeffrey P. Spencer, Robert G. M. Ogles, Olivia C. Rich, Virginia I. McCalley, Carmody K. Wilson, Rachel M. 2022-02-02 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8809605/ https://doi.org/10.1371/journal.pone.0252743 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8809605/ http://dx.doi.org/10.1371/journal.pone.0252743 © 2022 Cory et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. CC-BY PLoS One Research Article Text 2022 ftpubmed https://doi.org/10.1371/journal.pone.0252743 2022-02-06T02:19:02Z The mechanisms controlling the extraordinarily slow carbon (C) mineralization rates characteristic of Sphagnum-rich peatlands (“bogs”) are not fully understood, despite decades of research on this topic. Soluble phenolic compounds have been invoked as potentially significant contributors to bog peat recalcitrance due to their affinity to slow microbial metabolism and cell growth. Despite this potentially significant role, the effects of soluble phenolic compounds on bog peat C mineralization remain unclear. We analyzed this effect by manipulating the concentration of free soluble phenolics in anaerobic bog and fen peat incubations using water-soluble polyvinylpyrrolidone (“PVP”), a compound that binds with and inactivates phenolics, preventing phenolic-enzyme interactions. CO(2) and CH(4) production rates (end-products of anaerobic C mineralization) generally correlated positively with PVP concentration following Michaelis-Menten (M.M.) saturation functions. Using M.M. parameters, we estimated that the extent to which phenolics inhibit anaerobic CO(2) production was significantly higher in the bog—62 ± 16%—than the fen—14 ± 4%. This difference was found to be more substantial with regards to methane production—wherein phenolic inhibition for the bog was estimated at 54 ± 19%, while the fen demonstrated no apparent inhibition. Consistent with this habitat difference, we observed significantly higher soluble phenolic content in bog vs. fen pore-water. Together, these findings suggest that soluble phenolics could contribute to bogs’ extraordinary recalcitrance and high (relative to other peatland habitats) CO(2):CH(4) production ratios. Text permafrost PubMed Central (PMC) PLOS ONE 17 2 e0252743 |
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Research Article Cory, Alexandra B. Chanton, Jeffrey P. Spencer, Robert G. M. Ogles, Olivia C. Rich, Virginia I. McCalley, Carmody K. Wilson, Rachel M. Quantifying the inhibitory impact of soluble phenolics on anaerobic carbon mineralization in a thawing permafrost peatland |
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Research Article |
description |
The mechanisms controlling the extraordinarily slow carbon (C) mineralization rates characteristic of Sphagnum-rich peatlands (“bogs”) are not fully understood, despite decades of research on this topic. Soluble phenolic compounds have been invoked as potentially significant contributors to bog peat recalcitrance due to their affinity to slow microbial metabolism and cell growth. Despite this potentially significant role, the effects of soluble phenolic compounds on bog peat C mineralization remain unclear. We analyzed this effect by manipulating the concentration of free soluble phenolics in anaerobic bog and fen peat incubations using water-soluble polyvinylpyrrolidone (“PVP”), a compound that binds with and inactivates phenolics, preventing phenolic-enzyme interactions. CO(2) and CH(4) production rates (end-products of anaerobic C mineralization) generally correlated positively with PVP concentration following Michaelis-Menten (M.M.) saturation functions. Using M.M. parameters, we estimated that the extent to which phenolics inhibit anaerobic CO(2) production was significantly higher in the bog—62 ± 16%—than the fen—14 ± 4%. This difference was found to be more substantial with regards to methane production—wherein phenolic inhibition for the bog was estimated at 54 ± 19%, while the fen demonstrated no apparent inhibition. Consistent with this habitat difference, we observed significantly higher soluble phenolic content in bog vs. fen pore-water. Together, these findings suggest that soluble phenolics could contribute to bogs’ extraordinary recalcitrance and high (relative to other peatland habitats) CO(2):CH(4) production ratios. |
format |
Text |
author |
Cory, Alexandra B. Chanton, Jeffrey P. Spencer, Robert G. M. Ogles, Olivia C. Rich, Virginia I. McCalley, Carmody K. Wilson, Rachel M. |
author_facet |
Cory, Alexandra B. Chanton, Jeffrey P. Spencer, Robert G. M. Ogles, Olivia C. Rich, Virginia I. McCalley, Carmody K. Wilson, Rachel M. |
author_sort |
Cory, Alexandra B. |
title |
Quantifying the inhibitory impact of soluble phenolics on anaerobic carbon mineralization in a thawing permafrost peatland |
title_short |
Quantifying the inhibitory impact of soluble phenolics on anaerobic carbon mineralization in a thawing permafrost peatland |
title_full |
Quantifying the inhibitory impact of soluble phenolics on anaerobic carbon mineralization in a thawing permafrost peatland |
title_fullStr |
Quantifying the inhibitory impact of soluble phenolics on anaerobic carbon mineralization in a thawing permafrost peatland |
title_full_unstemmed |
Quantifying the inhibitory impact of soluble phenolics on anaerobic carbon mineralization in a thawing permafrost peatland |
title_sort |
quantifying the inhibitory impact of soluble phenolics on anaerobic carbon mineralization in a thawing permafrost peatland |
publisher |
Public Library of Science |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8809605/ https://doi.org/10.1371/journal.pone.0252743 |
genre |
permafrost |
genre_facet |
permafrost |
op_source |
PLoS One |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8809605/ http://dx.doi.org/10.1371/journal.pone.0252743 |
op_rights |
© 2022 Cory et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1371/journal.pone.0252743 |
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PLOS ONE |
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17 |
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2 |
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e0252743 |
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