Novel Insights into Dimethylsulfoniopropionate Catabolism by Cultivable Bacteria in the Arctic Kongsfjorden
Dimethylsulfoniopropionate (DMSP) is one of the most abundant organic sulfur compounds in the oceans, which is mainly degraded by bacteria through two pathways, a cleavage pathway and a demethylation pathway. Its volatile catabolites dimethyl sulfide (DMS) and methanethiol (MT) in these pathways pla...
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American Society for Microbiology
2022
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8788697/ http://www.ncbi.nlm.nih.gov/pubmed/34788071 https://doi.org/10.1128/AEM.01806-21 |
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ftpubmed:oai:pubmedcentral.nih.gov:8788697 2023-05-15T14:51:53+02:00 Novel Insights into Dimethylsulfoniopropionate Catabolism by Cultivable Bacteria in the Arctic Kongsfjorden Zhang, Shan Cao, Hai-Yan Zhang, Nan Teng, Zhao-Jie Yu, Yang Wang, Zhi-Bin Wang, Peng Fu, Hui-Hui Chen, Xiu-Lan Zhang, Yu-Zhong Li, Chun-Yang 2022-01-25 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8788697/ http://www.ncbi.nlm.nih.gov/pubmed/34788071 https://doi.org/10.1128/AEM.01806-21 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8788697/ http://www.ncbi.nlm.nih.gov/pubmed/34788071 http://dx.doi.org/10.1128/AEM.01806-21 Copyright © 2022 American Society for Microbiology. https://doi.org/10.1128/ASMCopyrightv2All Rights Reserved (https://doi.org/10.1128/ASMCopyrightv2) . Appl Environ Microbiol Environmental Microbiology Text 2022 ftpubmed https://doi.org/10.1128/AEM.01806-21 2022-07-31T00:32:43Z Dimethylsulfoniopropionate (DMSP) is one of the most abundant organic sulfur compounds in the oceans, which is mainly degraded by bacteria through two pathways, a cleavage pathway and a demethylation pathway. Its volatile catabolites dimethyl sulfide (DMS) and methanethiol (MT) in these pathways play important roles in the global sulfur cycle and have potential influences on the global climate. Intense DMS/DMSP cycling occurs in the Arctic. However, little is known about the diversity of cultivable DMSP-catabolizing bacteria in the Arctic and how they catabolize DMSP. Here, we screened DMSP-catabolizing bacteria from Arctic samples and found that bacteria of four genera (Psychrobacter, Pseudoalteromonas, Alteromonas, and Vibrio) could grow with DMSP as the sole carbon source, among which Psychrobacter and Pseudoalteromonas are predominant. Four representative strains (Psychrobacter sp. K31L, Pseudoalteromonas sp. K222D, Alteromonas sp. K632G, and Vibrio sp. G41H) from different genera were selected to probe their DMSP catabolic pathways. All these strains produce DMS and MT simultaneously during their growth on DMSP, indicating that all strains likely possess the two DMSP catabolic pathways. On the basis of genomic and biochemical analyses, the DMSP catabolic pathways in these strains were proposed. Bioinformatic analysis indicated that most Psychrobacter and Vibrio bacteria have the potential to catabolize DMSP via the demethylation pathway and that only a small portion of Psychrobacter strains may catabolize DMSP via the cleavage pathway. This study provides novel insights into DMSP catabolism in marine bacteria. IMPORTANCE Dimethylsulfoniopropionate (DMSP) is abundant in the oceans. The catabolism of DMSP is an important step of the global sulfur cycle. Although Gammaproteobacteria are widespread in the oceans, the contribution of Gammaproteobacteria in global DMSP catabolism is not fully understood. Here, we found that bacteria of four genera belonging to Gammaproteobacteria (Psychrobacter, ... Text Arctic Kongsfjord* Kongsfjorden PubMed Central (PMC) Arctic Applied and Environmental Microbiology 88 2 |
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PubMed Central (PMC) |
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ftpubmed |
language |
English |
topic |
Environmental Microbiology |
spellingShingle |
Environmental Microbiology Zhang, Shan Cao, Hai-Yan Zhang, Nan Teng, Zhao-Jie Yu, Yang Wang, Zhi-Bin Wang, Peng Fu, Hui-Hui Chen, Xiu-Lan Zhang, Yu-Zhong Li, Chun-Yang Novel Insights into Dimethylsulfoniopropionate Catabolism by Cultivable Bacteria in the Arctic Kongsfjorden |
topic_facet |
Environmental Microbiology |
description |
Dimethylsulfoniopropionate (DMSP) is one of the most abundant organic sulfur compounds in the oceans, which is mainly degraded by bacteria through two pathways, a cleavage pathway and a demethylation pathway. Its volatile catabolites dimethyl sulfide (DMS) and methanethiol (MT) in these pathways play important roles in the global sulfur cycle and have potential influences on the global climate. Intense DMS/DMSP cycling occurs in the Arctic. However, little is known about the diversity of cultivable DMSP-catabolizing bacteria in the Arctic and how they catabolize DMSP. Here, we screened DMSP-catabolizing bacteria from Arctic samples and found that bacteria of four genera (Psychrobacter, Pseudoalteromonas, Alteromonas, and Vibrio) could grow with DMSP as the sole carbon source, among which Psychrobacter and Pseudoalteromonas are predominant. Four representative strains (Psychrobacter sp. K31L, Pseudoalteromonas sp. K222D, Alteromonas sp. K632G, and Vibrio sp. G41H) from different genera were selected to probe their DMSP catabolic pathways. All these strains produce DMS and MT simultaneously during their growth on DMSP, indicating that all strains likely possess the two DMSP catabolic pathways. On the basis of genomic and biochemical analyses, the DMSP catabolic pathways in these strains were proposed. Bioinformatic analysis indicated that most Psychrobacter and Vibrio bacteria have the potential to catabolize DMSP via the demethylation pathway and that only a small portion of Psychrobacter strains may catabolize DMSP via the cleavage pathway. This study provides novel insights into DMSP catabolism in marine bacteria. IMPORTANCE Dimethylsulfoniopropionate (DMSP) is abundant in the oceans. The catabolism of DMSP is an important step of the global sulfur cycle. Although Gammaproteobacteria are widespread in the oceans, the contribution of Gammaproteobacteria in global DMSP catabolism is not fully understood. Here, we found that bacteria of four genera belonging to Gammaproteobacteria (Psychrobacter, ... |
format |
Text |
author |
Zhang, Shan Cao, Hai-Yan Zhang, Nan Teng, Zhao-Jie Yu, Yang Wang, Zhi-Bin Wang, Peng Fu, Hui-Hui Chen, Xiu-Lan Zhang, Yu-Zhong Li, Chun-Yang |
author_facet |
Zhang, Shan Cao, Hai-Yan Zhang, Nan Teng, Zhao-Jie Yu, Yang Wang, Zhi-Bin Wang, Peng Fu, Hui-Hui Chen, Xiu-Lan Zhang, Yu-Zhong Li, Chun-Yang |
author_sort |
Zhang, Shan |
title |
Novel Insights into Dimethylsulfoniopropionate Catabolism by Cultivable Bacteria in the Arctic Kongsfjorden |
title_short |
Novel Insights into Dimethylsulfoniopropionate Catabolism by Cultivable Bacteria in the Arctic Kongsfjorden |
title_full |
Novel Insights into Dimethylsulfoniopropionate Catabolism by Cultivable Bacteria in the Arctic Kongsfjorden |
title_fullStr |
Novel Insights into Dimethylsulfoniopropionate Catabolism by Cultivable Bacteria in the Arctic Kongsfjorden |
title_full_unstemmed |
Novel Insights into Dimethylsulfoniopropionate Catabolism by Cultivable Bacteria in the Arctic Kongsfjorden |
title_sort |
novel insights into dimethylsulfoniopropionate catabolism by cultivable bacteria in the arctic kongsfjorden |
publisher |
American Society for Microbiology |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8788697/ http://www.ncbi.nlm.nih.gov/pubmed/34788071 https://doi.org/10.1128/AEM.01806-21 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Kongsfjord* Kongsfjorden |
genre_facet |
Arctic Kongsfjord* Kongsfjorden |
op_source |
Appl Environ Microbiol |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8788697/ http://www.ncbi.nlm.nih.gov/pubmed/34788071 http://dx.doi.org/10.1128/AEM.01806-21 |
op_rights |
Copyright © 2022 American Society for Microbiology. https://doi.org/10.1128/ASMCopyrightv2All Rights Reserved (https://doi.org/10.1128/ASMCopyrightv2) . |
op_doi |
https://doi.org/10.1128/AEM.01806-21 |
container_title |
Applied and Environmental Microbiology |
container_volume |
88 |
container_issue |
2 |
_version_ |
1766323027527598080 |