Multilocus sequence based identification and adaptational strategies of Pseudomonas sp. from the supraglacial site of Sikkim Himalaya
Microorganisms inhabiting the supraglacial ice are biotechnologically significant as they are equipped with unique adaptive features in response to extreme environmental conditions of high ultraviolet radiations and frequent freeze-thaw. In the current study, we obtained eleven strains of Pseudomona...
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ftpubmed:oai:pubmedcentral.nih.gov:8786180 2023-05-15T13:54:22+02:00 Multilocus sequence based identification and adaptational strategies of Pseudomonas sp. from the supraglacial site of Sikkim Himalaya Mukhia, Srijana Kumar, Anil Kumari, Poonam Kumar, Rakshak Kumar, Sanjay 2022-01-24 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8786180/ http://www.ncbi.nlm.nih.gov/pubmed/35073328 https://doi.org/10.1371/journal.pone.0261178 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8786180/ http://www.ncbi.nlm.nih.gov/pubmed/35073328 http://dx.doi.org/10.1371/journal.pone.0261178 © 2022 Mukhia et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. CC-BY PLoS One Research Article Text 2022 ftpubmed https://doi.org/10.1371/journal.pone.0261178 2022-01-30T01:38:06Z Microorganisms inhabiting the supraglacial ice are biotechnologically significant as they are equipped with unique adaptive features in response to extreme environmental conditions of high ultraviolet radiations and frequent freeze-thaw. In the current study, we obtained eleven strains of Pseudomonas from the East Rathong supraglacial site in Sikkim Himalaya that showed taxonomic ambiguity in terms of species affiliation. Being one of the most complex and diverse genera, deciphering the correct taxonomy of Pseudomonas species has always been challenging. So, we conducted multilocus sequence analysis (MLSA) using five housekeeping genes, which concluded the taxonomic assignment of these strains to Pseudomonas antarctica. This was further supported by the lesser mean genetic distances with P. antarctica (0.73%) compared to P. fluorescens (3.65%), and highest ANI value of ~99 and dDDH value of 91.2 of the representative strains with P. antarctica PAMC 27494. We examined the multi-tolerance abilities of these eleven Pseudomonas strains. Indeed the studied strains displayed significant tolerance to freezing for 96 hours compared to the mesophilic control strain, while except for four strains, seven strains exhibited noteworthy tolerance to UV-C radiations. The genome-based findings revealed many cold and radiation resistance-associated genes that supported the physiological findings. Further, the bacterial strains produced two or more cold-active enzymes in plate-based assays. Owing to the polyadaptational attributes, the strains ERGC3:01 and ERGC3:05 could be most promising for bioprospection. Text Antarc* Antarctica PubMed Central (PMC) PLOS ONE 17 1 e0261178 |
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Research Article Mukhia, Srijana Kumar, Anil Kumari, Poonam Kumar, Rakshak Kumar, Sanjay Multilocus sequence based identification and adaptational strategies of Pseudomonas sp. from the supraglacial site of Sikkim Himalaya |
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Research Article |
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Microorganisms inhabiting the supraglacial ice are biotechnologically significant as they are equipped with unique adaptive features in response to extreme environmental conditions of high ultraviolet radiations and frequent freeze-thaw. In the current study, we obtained eleven strains of Pseudomonas from the East Rathong supraglacial site in Sikkim Himalaya that showed taxonomic ambiguity in terms of species affiliation. Being one of the most complex and diverse genera, deciphering the correct taxonomy of Pseudomonas species has always been challenging. So, we conducted multilocus sequence analysis (MLSA) using five housekeeping genes, which concluded the taxonomic assignment of these strains to Pseudomonas antarctica. This was further supported by the lesser mean genetic distances with P. antarctica (0.73%) compared to P. fluorescens (3.65%), and highest ANI value of ~99 and dDDH value of 91.2 of the representative strains with P. antarctica PAMC 27494. We examined the multi-tolerance abilities of these eleven Pseudomonas strains. Indeed the studied strains displayed significant tolerance to freezing for 96 hours compared to the mesophilic control strain, while except for four strains, seven strains exhibited noteworthy tolerance to UV-C radiations. The genome-based findings revealed many cold and radiation resistance-associated genes that supported the physiological findings. Further, the bacterial strains produced two or more cold-active enzymes in plate-based assays. Owing to the polyadaptational attributes, the strains ERGC3:01 and ERGC3:05 could be most promising for bioprospection. |
format |
Text |
author |
Mukhia, Srijana Kumar, Anil Kumari, Poonam Kumar, Rakshak Kumar, Sanjay |
author_facet |
Mukhia, Srijana Kumar, Anil Kumari, Poonam Kumar, Rakshak Kumar, Sanjay |
author_sort |
Mukhia, Srijana |
title |
Multilocus sequence based identification and adaptational strategies of Pseudomonas sp. from the supraglacial site of Sikkim Himalaya |
title_short |
Multilocus sequence based identification and adaptational strategies of Pseudomonas sp. from the supraglacial site of Sikkim Himalaya |
title_full |
Multilocus sequence based identification and adaptational strategies of Pseudomonas sp. from the supraglacial site of Sikkim Himalaya |
title_fullStr |
Multilocus sequence based identification and adaptational strategies of Pseudomonas sp. from the supraglacial site of Sikkim Himalaya |
title_full_unstemmed |
Multilocus sequence based identification and adaptational strategies of Pseudomonas sp. from the supraglacial site of Sikkim Himalaya |
title_sort |
multilocus sequence based identification and adaptational strategies of pseudomonas sp. from the supraglacial site of sikkim himalaya |
publisher |
Public Library of Science |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8786180/ http://www.ncbi.nlm.nih.gov/pubmed/35073328 https://doi.org/10.1371/journal.pone.0261178 |
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Antarc* Antarctica |
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Antarc* Antarctica |
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PLoS One |
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8786180/ http://www.ncbi.nlm.nih.gov/pubmed/35073328 http://dx.doi.org/10.1371/journal.pone.0261178 |
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© 2022 Mukhia et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
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https://doi.org/10.1371/journal.pone.0261178 |
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