Assessing rodents as carriers of pathogenic Leptospira species in the U.S. Virgin Islands and their risk to animal and public health
Leptospirosis is a global zoonotic disease caused by pathogenic bacteria of the genus Leptospira. We sought to determine if rodents in U.S. Virgin Islands (USVI) are carriers of Leptospira. In total, 140 rodents were sampled, including 112 Mus musculus and 28 Rattus rattus. A positive carrier status...
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ftpubmed:oai:pubmedcentral.nih.gov:8782869 2023-05-15T18:05:17+02:00 Assessing rodents as carriers of pathogenic Leptospira species in the U.S. Virgin Islands and their risk to animal and public health Hamond, Camila Browne, A. Springer de Wilde, Leah H. Hornsby, Richard L. LeCount, Karen Anderson, Tammy Stuber, Tod Cranford, Hannah M. Browne, Stephanie K. Blanchard, Gerard Horner, David Taylor, Marissa L. Evans, Michael Angeli, Nicole F. Roth, Joseph Bisgard, Kristine M. Salzer, Johanna S. Schafer, Ilana J. Ellis, Brett R. Alt, David P. Schlater, Linda Nally, Jarlath E. Ellis, Esther M. 2022-01-21 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8782869/ http://www.ncbi.nlm.nih.gov/pubmed/35064157 https://doi.org/10.1038/s41598-022-04846-3 en eng Nature Publishing Group UK http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8782869/ http://www.ncbi.nlm.nih.gov/pubmed/35064157 http://dx.doi.org/10.1038/s41598-022-04846-3 © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . CC-BY Sci Rep Article Text 2022 ftpubmed https://doi.org/10.1038/s41598-022-04846-3 2022-01-30T01:33:46Z Leptospirosis is a global zoonotic disease caused by pathogenic bacteria of the genus Leptospira. We sought to determine if rodents in U.S. Virgin Islands (USVI) are carriers of Leptospira. In total, 140 rodents were sampled, including 112 Mus musculus and 28 Rattus rattus. A positive carrier status was identified for 64/140 (45.7%); 49 (35.0%) were positive by dark-field microscopy, 60 (42.9%) by culture, 63 (45.0%) by fluorescent antibody testing, and 61 (43.6%) by real-time polymerase chain reaction (rtPCR). Molecular typing indicated that 48 isolates were L. borgpetersenii and 3 were L. kirschneri; the remaining nine comprised mixed species. In the single culture-negative sample that was rtPCR positive, genotyping directly from the kidney identified L. interrogans. Serotyping of L. borgpetersenii isolates identified serogroup Ballum and L. kirschneri isolates as serogroup Icterohaemorrhagiae. These results demonstrate that rodents are significant Leptospira carriers and adds to understanding the ecoepidemiology of leptospirosis in USVI. Text Rattus rattus PubMed Central (PMC) Scientific Reports 12 1 |
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Article Hamond, Camila Browne, A. Springer de Wilde, Leah H. Hornsby, Richard L. LeCount, Karen Anderson, Tammy Stuber, Tod Cranford, Hannah M. Browne, Stephanie K. Blanchard, Gerard Horner, David Taylor, Marissa L. Evans, Michael Angeli, Nicole F. Roth, Joseph Bisgard, Kristine M. Salzer, Johanna S. Schafer, Ilana J. Ellis, Brett R. Alt, David P. Schlater, Linda Nally, Jarlath E. Ellis, Esther M. Assessing rodents as carriers of pathogenic Leptospira species in the U.S. Virgin Islands and their risk to animal and public health |
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Leptospirosis is a global zoonotic disease caused by pathogenic bacteria of the genus Leptospira. We sought to determine if rodents in U.S. Virgin Islands (USVI) are carriers of Leptospira. In total, 140 rodents were sampled, including 112 Mus musculus and 28 Rattus rattus. A positive carrier status was identified for 64/140 (45.7%); 49 (35.0%) were positive by dark-field microscopy, 60 (42.9%) by culture, 63 (45.0%) by fluorescent antibody testing, and 61 (43.6%) by real-time polymerase chain reaction (rtPCR). Molecular typing indicated that 48 isolates were L. borgpetersenii and 3 were L. kirschneri; the remaining nine comprised mixed species. In the single culture-negative sample that was rtPCR positive, genotyping directly from the kidney identified L. interrogans. Serotyping of L. borgpetersenii isolates identified serogroup Ballum and L. kirschneri isolates as serogroup Icterohaemorrhagiae. These results demonstrate that rodents are significant Leptospira carriers and adds to understanding the ecoepidemiology of leptospirosis in USVI. |
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Text |
author |
Hamond, Camila Browne, A. Springer de Wilde, Leah H. Hornsby, Richard L. LeCount, Karen Anderson, Tammy Stuber, Tod Cranford, Hannah M. Browne, Stephanie K. Blanchard, Gerard Horner, David Taylor, Marissa L. Evans, Michael Angeli, Nicole F. Roth, Joseph Bisgard, Kristine M. Salzer, Johanna S. Schafer, Ilana J. Ellis, Brett R. Alt, David P. Schlater, Linda Nally, Jarlath E. Ellis, Esther M. |
author_facet |
Hamond, Camila Browne, A. Springer de Wilde, Leah H. Hornsby, Richard L. LeCount, Karen Anderson, Tammy Stuber, Tod Cranford, Hannah M. Browne, Stephanie K. Blanchard, Gerard Horner, David Taylor, Marissa L. Evans, Michael Angeli, Nicole F. Roth, Joseph Bisgard, Kristine M. Salzer, Johanna S. Schafer, Ilana J. Ellis, Brett R. Alt, David P. Schlater, Linda Nally, Jarlath E. Ellis, Esther M. |
author_sort |
Hamond, Camila |
title |
Assessing rodents as carriers of pathogenic Leptospira species in the U.S. Virgin Islands and their risk to animal and public health |
title_short |
Assessing rodents as carriers of pathogenic Leptospira species in the U.S. Virgin Islands and their risk to animal and public health |
title_full |
Assessing rodents as carriers of pathogenic Leptospira species in the U.S. Virgin Islands and their risk to animal and public health |
title_fullStr |
Assessing rodents as carriers of pathogenic Leptospira species in the U.S. Virgin Islands and their risk to animal and public health |
title_full_unstemmed |
Assessing rodents as carriers of pathogenic Leptospira species in the U.S. Virgin Islands and their risk to animal and public health |
title_sort |
assessing rodents as carriers of pathogenic leptospira species in the u.s. virgin islands and their risk to animal and public health |
publisher |
Nature Publishing Group UK |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8782869/ http://www.ncbi.nlm.nih.gov/pubmed/35064157 https://doi.org/10.1038/s41598-022-04846-3 |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
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Sci Rep |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8782869/ http://www.ncbi.nlm.nih.gov/pubmed/35064157 http://dx.doi.org/10.1038/s41598-022-04846-3 |
op_rights |
© The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
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CC-BY |
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https://doi.org/10.1038/s41598-022-04846-3 |
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