Strong and widespread cycloheximide resistance in Stichococcus-like eukaryotic algal taxa
This study was initiated following the serendipitous discovery of a unialgal culture of a Stichococcus-like green alga (Chlorophyta) newly isolated from soil collected on Signy Island (maritime Antarctica) in growth medium supplemented with 100 µg/mL cycloheximide (CHX, a widely used antibiotic acti...
Published in: | Scientific Reports |
---|---|
Main Authors: | , , , , , |
Format: | Text |
Language: | English |
Published: |
Nature Publishing Group UK
2022
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8776791/ http://www.ncbi.nlm.nih.gov/pubmed/35058560 https://doi.org/10.1038/s41598-022-05116-y |
id |
ftpubmed:oai:pubmedcentral.nih.gov:8776791 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:8776791 2023-05-15T13:54:22+02:00 Strong and widespread cycloheximide resistance in Stichococcus-like eukaryotic algal taxa Syuhada, Nur Hidayu Merican, Faradina Zaki, Syazana Broady, Paul A. Convey, Peter Muangmai, Narongrit 2022-01-20 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8776791/ http://www.ncbi.nlm.nih.gov/pubmed/35058560 https://doi.org/10.1038/s41598-022-05116-y en eng Nature Publishing Group UK http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8776791/ http://www.ncbi.nlm.nih.gov/pubmed/35058560 http://dx.doi.org/10.1038/s41598-022-05116-y © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . CC-BY Sci Rep Article Text 2022 ftpubmed https://doi.org/10.1038/s41598-022-05116-y 2022-01-30T01:32:21Z This study was initiated following the serendipitous discovery of a unialgal culture of a Stichococcus-like green alga (Chlorophyta) newly isolated from soil collected on Signy Island (maritime Antarctica) in growth medium supplemented with 100 µg/mL cycloheximide (CHX, a widely used antibiotic active against most eukaryotes). In order to test the generality of CHX resistance in taxa originally identified as members of Stichococcus (the detailed taxonomic relationships within this group of algae have been updated since our study took place), six strains were studied: two strains isolated from recent substrate collections from Signy Island (maritime Antarctica) (“Antarctica” 1 and “Antarctica” 2), one isolated from this island about 50 years ago (“Antarctica” 3) and single Arctic (“Arctic”), temperate (“Temperate”) and tropical (“Tropical”) strains. The sensitivity of each strain towards CHX was compared by determining the minimum inhibitory concentration (MIC), and growth rate and lag time when exposed to different CHX concentrations. All strains except “Temperate” were highly resistant to CHX (MIC > 1000 µg/mL), while “Temperate” was resistant to 62.5 µg/mL (a concentration still considerably greater than any previously reported for algae). All highly resistant strains showed no significant differences in growth rate between control and treatment (1000 µg/mL CHX) conditions. Morphological examination suggested that four strains were consistent with the description of the species Stichococcus bacillaris while the remaining two conformed to S. mirabilis. However, based on sequence analyses and the recently available phylogeny, only one strain, “Temperate”, was confirmed to be S. bacillaris, while “Tropical” represents the newly erected genus Tetratostichococcus, “Antarctica 1” Tritostichococcus, and “Antarctica 2”, “Antarctica 3” and “Arctic” Deuterostichococcus. Both phylogenetic and CHX sensitivity analyses suggest that CHX resistance is potentially widespread within this group of algae. Text Antarc* Antarctica Arctic Signy Island PubMed Central (PMC) Arctic Signy Island ENVELOPE(-45.595,-45.595,-60.708,-60.708) Scientific Reports 12 1 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Article |
spellingShingle |
Article Syuhada, Nur Hidayu Merican, Faradina Zaki, Syazana Broady, Paul A. Convey, Peter Muangmai, Narongrit Strong and widespread cycloheximide resistance in Stichococcus-like eukaryotic algal taxa |
topic_facet |
Article |
description |
This study was initiated following the serendipitous discovery of a unialgal culture of a Stichococcus-like green alga (Chlorophyta) newly isolated from soil collected on Signy Island (maritime Antarctica) in growth medium supplemented with 100 µg/mL cycloheximide (CHX, a widely used antibiotic active against most eukaryotes). In order to test the generality of CHX resistance in taxa originally identified as members of Stichococcus (the detailed taxonomic relationships within this group of algae have been updated since our study took place), six strains were studied: two strains isolated from recent substrate collections from Signy Island (maritime Antarctica) (“Antarctica” 1 and “Antarctica” 2), one isolated from this island about 50 years ago (“Antarctica” 3) and single Arctic (“Arctic”), temperate (“Temperate”) and tropical (“Tropical”) strains. The sensitivity of each strain towards CHX was compared by determining the minimum inhibitory concentration (MIC), and growth rate and lag time when exposed to different CHX concentrations. All strains except “Temperate” were highly resistant to CHX (MIC > 1000 µg/mL), while “Temperate” was resistant to 62.5 µg/mL (a concentration still considerably greater than any previously reported for algae). All highly resistant strains showed no significant differences in growth rate between control and treatment (1000 µg/mL CHX) conditions. Morphological examination suggested that four strains were consistent with the description of the species Stichococcus bacillaris while the remaining two conformed to S. mirabilis. However, based on sequence analyses and the recently available phylogeny, only one strain, “Temperate”, was confirmed to be S. bacillaris, while “Tropical” represents the newly erected genus Tetratostichococcus, “Antarctica 1” Tritostichococcus, and “Antarctica 2”, “Antarctica 3” and “Arctic” Deuterostichococcus. Both phylogenetic and CHX sensitivity analyses suggest that CHX resistance is potentially widespread within this group of algae. |
format |
Text |
author |
Syuhada, Nur Hidayu Merican, Faradina Zaki, Syazana Broady, Paul A. Convey, Peter Muangmai, Narongrit |
author_facet |
Syuhada, Nur Hidayu Merican, Faradina Zaki, Syazana Broady, Paul A. Convey, Peter Muangmai, Narongrit |
author_sort |
Syuhada, Nur Hidayu |
title |
Strong and widespread cycloheximide resistance in Stichococcus-like eukaryotic algal taxa |
title_short |
Strong and widespread cycloheximide resistance in Stichococcus-like eukaryotic algal taxa |
title_full |
Strong and widespread cycloheximide resistance in Stichococcus-like eukaryotic algal taxa |
title_fullStr |
Strong and widespread cycloheximide resistance in Stichococcus-like eukaryotic algal taxa |
title_full_unstemmed |
Strong and widespread cycloheximide resistance in Stichococcus-like eukaryotic algal taxa |
title_sort |
strong and widespread cycloheximide resistance in stichococcus-like eukaryotic algal taxa |
publisher |
Nature Publishing Group UK |
publishDate |
2022 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8776791/ http://www.ncbi.nlm.nih.gov/pubmed/35058560 https://doi.org/10.1038/s41598-022-05116-y |
long_lat |
ENVELOPE(-45.595,-45.595,-60.708,-60.708) |
geographic |
Arctic Signy Island |
geographic_facet |
Arctic Signy Island |
genre |
Antarc* Antarctica Arctic Signy Island |
genre_facet |
Antarc* Antarctica Arctic Signy Island |
op_source |
Sci Rep |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8776791/ http://www.ncbi.nlm.nih.gov/pubmed/35058560 http://dx.doi.org/10.1038/s41598-022-05116-y |
op_rights |
© The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1038/s41598-022-05116-y |
container_title |
Scientific Reports |
container_volume |
12 |
container_issue |
1 |
_version_ |
1766260111538388992 |