In Vitro and In Vivo Protective Effects of Lentil (Lens culinaris) Extract against Oxidative Stress-Induced Hepatotoxicity
Excessive oxidative stress plays a role in hepatotoxicity and the pathogenesis of hepatic diseases. In our previous study, the phenolic extract of beluga lentil (BLE) showed the most potent in vitro antioxidant activity among extracts of four common varieties of lentils; thus, we hypothesized that B...
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ftpubmed:oai:pubmedcentral.nih.gov:8746911 2023-05-15T15:41:53+02:00 In Vitro and In Vivo Protective Effects of Lentil (Lens culinaris) Extract against Oxidative Stress-Induced Hepatotoxicity Jung, Yeon-Seop Lee, So-Hee Chun, So Young Kim, Dae Hwan Jang, Byung Ik Han, Man-Hoon Lee, Syng-Ook 2021-12-23 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8746911/ http://www.ncbi.nlm.nih.gov/pubmed/35011291 https://doi.org/10.3390/molecules27010059 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8746911/ http://www.ncbi.nlm.nih.gov/pubmed/35011291 http://dx.doi.org/10.3390/molecules27010059 © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). CC-BY Molecules Article Text 2021 ftpubmed https://doi.org/10.3390/molecules27010059 2022-01-16T01:47:24Z Excessive oxidative stress plays a role in hepatotoxicity and the pathogenesis of hepatic diseases. In our previous study, the phenolic extract of beluga lentil (BLE) showed the most potent in vitro antioxidant activity among extracts of four common varieties of lentils; thus, we hypothesized that BLE might protect liver cells against oxidative stress-induced cytotoxicity. BLE was evaluated for its protective effects against oxidative stress-induced hepatotoxicity in AML12 mouse hepatocytes and BALB/c mice. H(2)O(2) treatment caused a marked decrease in cell viability; however, pretreatment with BLE (25–100 μg/mL) for 24 h significantly preserved the viability of H(2)O(2)-treated cells up to about 50% at 100 μg/mL. As expected, BLE dramatically reduced intracellular reactive oxygen species (ROS) levels in a dose-dependent manner in H(2)O(2)-treated cells. Further mechanistic studies demonstrated that BLE reduced cellular ROS levels, partly by increasing expression of antioxidant genes. Furthermore, pretreatment with BLE (400 mg/kg) for 2 weeks significantly reduced serum levels of alanine transaminase and triglyceride by about 49% and 40%, respectively, and increased the expression and activity of glutathione peroxidase in CCl(4)-treated BALB/c mice. These results suggest that BLE protects liver cells against oxidative stress, partly by inducing cellular antioxidant system; thus, it represents a potential source of nutraceuticals with hepatoprotective effects. Text Beluga Beluga* PubMed Central (PMC) Molecules 27 1 59 |
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Article Jung, Yeon-Seop Lee, So-Hee Chun, So Young Kim, Dae Hwan Jang, Byung Ik Han, Man-Hoon Lee, Syng-Ook In Vitro and In Vivo Protective Effects of Lentil (Lens culinaris) Extract against Oxidative Stress-Induced Hepatotoxicity |
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Excessive oxidative stress plays a role in hepatotoxicity and the pathogenesis of hepatic diseases. In our previous study, the phenolic extract of beluga lentil (BLE) showed the most potent in vitro antioxidant activity among extracts of four common varieties of lentils; thus, we hypothesized that BLE might protect liver cells against oxidative stress-induced cytotoxicity. BLE was evaluated for its protective effects against oxidative stress-induced hepatotoxicity in AML12 mouse hepatocytes and BALB/c mice. H(2)O(2) treatment caused a marked decrease in cell viability; however, pretreatment with BLE (25–100 μg/mL) for 24 h significantly preserved the viability of H(2)O(2)-treated cells up to about 50% at 100 μg/mL. As expected, BLE dramatically reduced intracellular reactive oxygen species (ROS) levels in a dose-dependent manner in H(2)O(2)-treated cells. Further mechanistic studies demonstrated that BLE reduced cellular ROS levels, partly by increasing expression of antioxidant genes. Furthermore, pretreatment with BLE (400 mg/kg) for 2 weeks significantly reduced serum levels of alanine transaminase and triglyceride by about 49% and 40%, respectively, and increased the expression and activity of glutathione peroxidase in CCl(4)-treated BALB/c mice. These results suggest that BLE protects liver cells against oxidative stress, partly by inducing cellular antioxidant system; thus, it represents a potential source of nutraceuticals with hepatoprotective effects. |
format |
Text |
author |
Jung, Yeon-Seop Lee, So-Hee Chun, So Young Kim, Dae Hwan Jang, Byung Ik Han, Man-Hoon Lee, Syng-Ook |
author_facet |
Jung, Yeon-Seop Lee, So-Hee Chun, So Young Kim, Dae Hwan Jang, Byung Ik Han, Man-Hoon Lee, Syng-Ook |
author_sort |
Jung, Yeon-Seop |
title |
In Vitro and In Vivo Protective Effects of Lentil (Lens culinaris) Extract against Oxidative Stress-Induced Hepatotoxicity |
title_short |
In Vitro and In Vivo Protective Effects of Lentil (Lens culinaris) Extract against Oxidative Stress-Induced Hepatotoxicity |
title_full |
In Vitro and In Vivo Protective Effects of Lentil (Lens culinaris) Extract against Oxidative Stress-Induced Hepatotoxicity |
title_fullStr |
In Vitro and In Vivo Protective Effects of Lentil (Lens culinaris) Extract against Oxidative Stress-Induced Hepatotoxicity |
title_full_unstemmed |
In Vitro and In Vivo Protective Effects of Lentil (Lens culinaris) Extract against Oxidative Stress-Induced Hepatotoxicity |
title_sort |
in vitro and in vivo protective effects of lentil (lens culinaris) extract against oxidative stress-induced hepatotoxicity |
publisher |
MDPI |
publishDate |
2021 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8746911/ http://www.ncbi.nlm.nih.gov/pubmed/35011291 https://doi.org/10.3390/molecules27010059 |
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Beluga Beluga* |
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Beluga Beluga* |
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Molecules |
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8746911/ http://www.ncbi.nlm.nih.gov/pubmed/35011291 http://dx.doi.org/10.3390/molecules27010059 |
op_rights |
© 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
op_doi |
https://doi.org/10.3390/molecules27010059 |
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Molecules |
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27 |
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59 |
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