Marine pollutant exposures and human milk extracellular vesicle-microRNAs in a mother-infant cohort from the Faroe Islands
BACKGROUND/AIMS: Early life exposures to marine contaminants can adversely impact child health but modes of action are unclear. Human milk contains extracellular vesicles (EVs) that can transport biologically relevant cargo from mother to infant, including microRNAs (miRNAs), and may partly mediate...
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ftpubmed:oai:pubmedcentral.nih.gov:8742869 2023-05-15T16:11:01+02:00 Marine pollutant exposures and human milk extracellular vesicle-microRNAs in a mother-infant cohort from the Faroe Islands Kupsco, Allison Lee, Jenny Jyoung Prada, Diddier Valvi, Damaskini Hu, Lisa Petersen, Maria Skaalum Coull, Brent A. Weihe, Pal Grandjean, Philippe Baccarelli, Andrea A. 2021-11-20 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8742869/ http://www.ncbi.nlm.nih.gov/pubmed/34991248 https://doi.org/10.1016/j.envint.2021.106986 en eng http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8742869/ http://www.ncbi.nlm.nih.gov/pubmed/34991248 http://dx.doi.org/10.1016/j.envint.2021.106986 Environ Int Article Text 2021 ftpubmed https://doi.org/10.1016/j.envint.2021.106986 2023-01-08T01:37:16Z BACKGROUND/AIMS: Early life exposures to marine contaminants can adversely impact child health but modes of action are unclear. Human milk contains extracellular vesicles (EVs) that can transport biologically relevant cargo from mother to infant, including microRNAs (miRNAs), and may partly mediate the effects of pollutants on child health. However, the role of marine pollutants on miRNA expression in milk EVs is unexplored. METHODS: We isolated EV RNA from 333 milk samples collected between 2 and 74 days postpartum from a Faroese birth cohort born 1997–2000 and sequenced 2,083 miRNAs using a targeted library preparation method. We quantified five perfluoroalkyl substances (PFAS), pesticide metabolite p,p’-dichlorodiphenyldichloroethylene (DDE), and the sum of three major polychlorinated biphenyls (ΣPCBs) in maternal serum at 34 weeks of gestation and maternal hair total mercury (Hg) at birth. We used negative binomial regressions to estimate associations between individual pollutants and 418 reliably expressed EV-miRNAs adjusted for potential confounders. We performed sparse principal components (PCs) analysis to derive the first four components of the EV-miRNA data and examined associations between pollutants and PCs using Bayesian kernel machine regression (BKMR). RESULTS: We observed no associations between pollutants and individual EV-miRNA expression after controlling the false discovery rate at 0.1. However, BKMR suggested that Hg was positively associated with PC1 and negatively associated with PC3, while ΣPCBs was negatively associated with PC3, and two PFAS were associated with PC4. Exploration of PC loadings followed by pathway analyses suggested that miRNAs in PC1 (miR-200b-3p, miR-664a-3p, miR-6738–5p, miR-429, miR-1236–5p, miR-4464, and miR-30b-5p) may be related to Hg neurotoxicity, while remaining PCs require further research. CONCLUSIONS: Our findings suggest that groups of milk EV-miRNAs may better serve as environmental biomarkers than individual miRNAs. Future studies are needed to elucidate ... Text Faroe Islands PubMed Central (PMC) Faroe Islands Environment International 158 106986 |
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Article Kupsco, Allison Lee, Jenny Jyoung Prada, Diddier Valvi, Damaskini Hu, Lisa Petersen, Maria Skaalum Coull, Brent A. Weihe, Pal Grandjean, Philippe Baccarelli, Andrea A. Marine pollutant exposures and human milk extracellular vesicle-microRNAs in a mother-infant cohort from the Faroe Islands |
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description |
BACKGROUND/AIMS: Early life exposures to marine contaminants can adversely impact child health but modes of action are unclear. Human milk contains extracellular vesicles (EVs) that can transport biologically relevant cargo from mother to infant, including microRNAs (miRNAs), and may partly mediate the effects of pollutants on child health. However, the role of marine pollutants on miRNA expression in milk EVs is unexplored. METHODS: We isolated EV RNA from 333 milk samples collected between 2 and 74 days postpartum from a Faroese birth cohort born 1997–2000 and sequenced 2,083 miRNAs using a targeted library preparation method. We quantified five perfluoroalkyl substances (PFAS), pesticide metabolite p,p’-dichlorodiphenyldichloroethylene (DDE), and the sum of three major polychlorinated biphenyls (ΣPCBs) in maternal serum at 34 weeks of gestation and maternal hair total mercury (Hg) at birth. We used negative binomial regressions to estimate associations between individual pollutants and 418 reliably expressed EV-miRNAs adjusted for potential confounders. We performed sparse principal components (PCs) analysis to derive the first four components of the EV-miRNA data and examined associations between pollutants and PCs using Bayesian kernel machine regression (BKMR). RESULTS: We observed no associations between pollutants and individual EV-miRNA expression after controlling the false discovery rate at 0.1. However, BKMR suggested that Hg was positively associated with PC1 and negatively associated with PC3, while ΣPCBs was negatively associated with PC3, and two PFAS were associated with PC4. Exploration of PC loadings followed by pathway analyses suggested that miRNAs in PC1 (miR-200b-3p, miR-664a-3p, miR-6738–5p, miR-429, miR-1236–5p, miR-4464, and miR-30b-5p) may be related to Hg neurotoxicity, while remaining PCs require further research. CONCLUSIONS: Our findings suggest that groups of milk EV-miRNAs may better serve as environmental biomarkers than individual miRNAs. Future studies are needed to elucidate ... |
format |
Text |
author |
Kupsco, Allison Lee, Jenny Jyoung Prada, Diddier Valvi, Damaskini Hu, Lisa Petersen, Maria Skaalum Coull, Brent A. Weihe, Pal Grandjean, Philippe Baccarelli, Andrea A. |
author_facet |
Kupsco, Allison Lee, Jenny Jyoung Prada, Diddier Valvi, Damaskini Hu, Lisa Petersen, Maria Skaalum Coull, Brent A. Weihe, Pal Grandjean, Philippe Baccarelli, Andrea A. |
author_sort |
Kupsco, Allison |
title |
Marine pollutant exposures and human milk extracellular vesicle-microRNAs in a mother-infant cohort from the Faroe Islands |
title_short |
Marine pollutant exposures and human milk extracellular vesicle-microRNAs in a mother-infant cohort from the Faroe Islands |
title_full |
Marine pollutant exposures and human milk extracellular vesicle-microRNAs in a mother-infant cohort from the Faroe Islands |
title_fullStr |
Marine pollutant exposures and human milk extracellular vesicle-microRNAs in a mother-infant cohort from the Faroe Islands |
title_full_unstemmed |
Marine pollutant exposures and human milk extracellular vesicle-microRNAs in a mother-infant cohort from the Faroe Islands |
title_sort |
marine pollutant exposures and human milk extracellular vesicle-micrornas in a mother-infant cohort from the faroe islands |
publishDate |
2021 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8742869/ http://www.ncbi.nlm.nih.gov/pubmed/34991248 https://doi.org/10.1016/j.envint.2021.106986 |
geographic |
Faroe Islands |
geographic_facet |
Faroe Islands |
genre |
Faroe Islands |
genre_facet |
Faroe Islands |
op_source |
Environ Int |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8742869/ http://www.ncbi.nlm.nih.gov/pubmed/34991248 http://dx.doi.org/10.1016/j.envint.2021.106986 |
op_doi |
https://doi.org/10.1016/j.envint.2021.106986 |
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Environment International |
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158 |
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106986 |
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1765996135526170624 |