Metabolic flexibility of aerobic methanotrophs under anoxic conditions in Arctic lake sediments
Methane (CH(4)) emissions from Arctic lakes are a large and growing source of greenhouse gas to the atmosphere with critical implications for global climate. Because Arctic lakes are ice covered for much of the year, understanding the metabolic flexibility of methanotrophs under anoxic conditions wo...
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ftpubmed:oai:pubmedcentral.nih.gov:8692461 2023-05-15T14:51:51+02:00 Metabolic flexibility of aerobic methanotrophs under anoxic conditions in Arctic lake sediments He, Ruo Wang, Jing Pohlman, John W. Jia, Zhongjun Chu, Yi-Xuan Wooller, Matthew J. Leigh, Mary Beth 2021-07-09 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8692461/ http://www.ncbi.nlm.nih.gov/pubmed/34244610 https://doi.org/10.1038/s41396-021-01049-y en eng Nature Publishing Group UK http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8692461/ http://www.ncbi.nlm.nih.gov/pubmed/34244610 http://dx.doi.org/10.1038/s41396-021-01049-y © The Author(s), under exclusive licence to International Society for Microbial Ecology 2021 ISME J Article Text 2021 ftpubmed https://doi.org/10.1038/s41396-021-01049-y 2023-01-08T01:34:32Z Methane (CH(4)) emissions from Arctic lakes are a large and growing source of greenhouse gas to the atmosphere with critical implications for global climate. Because Arctic lakes are ice covered for much of the year, understanding the metabolic flexibility of methanotrophs under anoxic conditions would aid in characterizing the mechanisms responsible for limiting CH(4) emissions from high-latitude regions. Using sediments from an active CH(4) seep in Lake Qalluuraq, Alaska, we conducted DNA-based stable isotope probing (SIP) in anoxic mesocosms and found that aerobic Gammaproteobacterial methanotrophs dominated in assimilating CH(4). Aerobic methanotrophs were also detected down to 70 cm deep in sediments at the seep site, where anoxic conditions persist. Metagenomic analyses of the heavy DNA from (13)CH(4)-SIP incubations showed that these aerobic methanotrophs had the capacity to generate intermediates such as methanol, formaldehyde, and formate from CH(4) oxidation and to oxidize formaldehyde in the tetrahydromethanopterin (H(4)MPT)-dependent pathway under anoxic conditions. The high levels of Fe present in sediments, combined with Fe and CH(4) profiles in the persistent CH(4) seep site, suggested that oxidation of CH(4), or, more specifically, its intermediates such as methanol and formaldehyde might be coupled to iron reduction. Aerobic methanotrophs also possessed genes associated with nitrogen and hydrogen metabolism, which might provide potentially alternative energy conservation options under anoxic conditions. These results expand the known metabolic spectrum of aerobic methanotrophs under anoxic conditions and necessitate the re-assessment of the mechanisms underlying CH(4) oxidation in the Arctic, especially under lakes that experience extended O(2) limitations during ice cover. Text Arctic Alaska PubMed Central (PMC) Arctic Arctic Lake ENVELOPE(-130.826,-130.826,57.231,57.231) The ISME Journal 16 1 78 90 |
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Article He, Ruo Wang, Jing Pohlman, John W. Jia, Zhongjun Chu, Yi-Xuan Wooller, Matthew J. Leigh, Mary Beth Metabolic flexibility of aerobic methanotrophs under anoxic conditions in Arctic lake sediments |
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Methane (CH(4)) emissions from Arctic lakes are a large and growing source of greenhouse gas to the atmosphere with critical implications for global climate. Because Arctic lakes are ice covered for much of the year, understanding the metabolic flexibility of methanotrophs under anoxic conditions would aid in characterizing the mechanisms responsible for limiting CH(4) emissions from high-latitude regions. Using sediments from an active CH(4) seep in Lake Qalluuraq, Alaska, we conducted DNA-based stable isotope probing (SIP) in anoxic mesocosms and found that aerobic Gammaproteobacterial methanotrophs dominated in assimilating CH(4). Aerobic methanotrophs were also detected down to 70 cm deep in sediments at the seep site, where anoxic conditions persist. Metagenomic analyses of the heavy DNA from (13)CH(4)-SIP incubations showed that these aerobic methanotrophs had the capacity to generate intermediates such as methanol, formaldehyde, and formate from CH(4) oxidation and to oxidize formaldehyde in the tetrahydromethanopterin (H(4)MPT)-dependent pathway under anoxic conditions. The high levels of Fe present in sediments, combined with Fe and CH(4) profiles in the persistent CH(4) seep site, suggested that oxidation of CH(4), or, more specifically, its intermediates such as methanol and formaldehyde might be coupled to iron reduction. Aerobic methanotrophs also possessed genes associated with nitrogen and hydrogen metabolism, which might provide potentially alternative energy conservation options under anoxic conditions. These results expand the known metabolic spectrum of aerobic methanotrophs under anoxic conditions and necessitate the re-assessment of the mechanisms underlying CH(4) oxidation in the Arctic, especially under lakes that experience extended O(2) limitations during ice cover. |
format |
Text |
author |
He, Ruo Wang, Jing Pohlman, John W. Jia, Zhongjun Chu, Yi-Xuan Wooller, Matthew J. Leigh, Mary Beth |
author_facet |
He, Ruo Wang, Jing Pohlman, John W. Jia, Zhongjun Chu, Yi-Xuan Wooller, Matthew J. Leigh, Mary Beth |
author_sort |
He, Ruo |
title |
Metabolic flexibility of aerobic methanotrophs under anoxic conditions in Arctic lake sediments |
title_short |
Metabolic flexibility of aerobic methanotrophs under anoxic conditions in Arctic lake sediments |
title_full |
Metabolic flexibility of aerobic methanotrophs under anoxic conditions in Arctic lake sediments |
title_fullStr |
Metabolic flexibility of aerobic methanotrophs under anoxic conditions in Arctic lake sediments |
title_full_unstemmed |
Metabolic flexibility of aerobic methanotrophs under anoxic conditions in Arctic lake sediments |
title_sort |
metabolic flexibility of aerobic methanotrophs under anoxic conditions in arctic lake sediments |
publisher |
Nature Publishing Group UK |
publishDate |
2021 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8692461/ http://www.ncbi.nlm.nih.gov/pubmed/34244610 https://doi.org/10.1038/s41396-021-01049-y |
long_lat |
ENVELOPE(-130.826,-130.826,57.231,57.231) |
geographic |
Arctic Arctic Lake |
geographic_facet |
Arctic Arctic Lake |
genre |
Arctic Alaska |
genre_facet |
Arctic Alaska |
op_source |
ISME J |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8692461/ http://www.ncbi.nlm.nih.gov/pubmed/34244610 http://dx.doi.org/10.1038/s41396-021-01049-y |
op_rights |
© The Author(s), under exclusive licence to International Society for Microbial Ecology 2021 |
op_doi |
https://doi.org/10.1038/s41396-021-01049-y |
container_title |
The ISME Journal |
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16 |
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1 |
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78 |
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90 |
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1766322997114699776 |