Transcriptomics Integrated With Metabolomics Reveal the Effects of Ultraviolet-B Radiation on Flavonoid Biosynthesis in Antarctic Moss
Bryophytes are the dominant vegetation in the Antarctic continent. They have suffered more unpleasant ultraviolet radiation due to the Antarctic ozone layer destruction. However, it remains unclear about the molecular mechanism of Antarctic moss acclimation to UV-B light. Here, the transcriptomics a...
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ftpubmed:oai:pubmedcentral.nih.gov:8692278 2023-05-15T13:31:23+02:00 Transcriptomics Integrated With Metabolomics Reveal the Effects of Ultraviolet-B Radiation on Flavonoid Biosynthesis in Antarctic Moss Liu, Shenghao Fang, Shuo Liu, Chenlin Zhao, Linlin Cong, Bailin Zhang, Zhaohui 2021-12-08 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8692278/ https://doi.org/10.3389/fpls.2021.788377 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8692278/ http://dx.doi.org/10.3389/fpls.2021.788377 Copyright © 2021 Liu, Fang, Liu, Zhao, Cong and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. CC-BY Front Plant Sci Plant Science Text 2021 ftpubmed https://doi.org/10.3389/fpls.2021.788377 2021-12-26T01:45:09Z Bryophytes are the dominant vegetation in the Antarctic continent. They have suffered more unpleasant ultraviolet radiation due to the Antarctic ozone layer destruction. However, it remains unclear about the molecular mechanism of Antarctic moss acclimation to UV-B light. Here, the transcriptomics and metabolomics approaches were conducted to uncover transcriptional and metabolic profiling of the Antarctic moss Leptobryum pyriforme under UV-B radiation. Totally, 67,290 unigenes with N(50) length of 2,055 bp were assembled. Of them, 1,594 unigenes were significantly up-regulated and 3353 unigenes were markedly down-regulated under UV-B radiation. These differentially expressed genes (DEGs) involved in UV-B signaling, flavonoid biosynthesis, ROS scavenging, and DNA repair. In addition, a total of 531 metabolites were detected, while flavonoids and anthocyanins accounted for 10.36% of the total compounds. There were 49 upregulated metabolites and 41 downregulated metabolites under UV-B radiation. Flavonoids were the most significantly changed metabolites. qPCR analysis showed that UVR8-COP1-HY5 signaling pathway genes and photolyase genes (i.e., LpUVR3, LpPHR1, and LpDPL) were significantly up-regulated under UV-B light. In addition, the expression levels of JA signaling pathway-related genes (i.e., OPR and JAZ) and flavonoid biosynthesis-related genes were also significantly increased under UV-B radiation. The integrative data analysis showed that UVR8-mediated signaling, jasmonate signaling, flavonoid biosynthesis pathway and DNA repair system might contribute to L. pyriforme acclimating to UV-B radiation. Therefore, these findings present a novel knowledge for understanding the adaption of Antarctic moss to polar environments and provide a foundation for assessing the impact of global climate change on Antarctic land plants. Text Antarc* Antarctic PubMed Central (PMC) Antarctic The Antarctic Frontiers in Plant Science 12 |
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language |
English |
topic |
Plant Science |
spellingShingle |
Plant Science Liu, Shenghao Fang, Shuo Liu, Chenlin Zhao, Linlin Cong, Bailin Zhang, Zhaohui Transcriptomics Integrated With Metabolomics Reveal the Effects of Ultraviolet-B Radiation on Flavonoid Biosynthesis in Antarctic Moss |
topic_facet |
Plant Science |
description |
Bryophytes are the dominant vegetation in the Antarctic continent. They have suffered more unpleasant ultraviolet radiation due to the Antarctic ozone layer destruction. However, it remains unclear about the molecular mechanism of Antarctic moss acclimation to UV-B light. Here, the transcriptomics and metabolomics approaches were conducted to uncover transcriptional and metabolic profiling of the Antarctic moss Leptobryum pyriforme under UV-B radiation. Totally, 67,290 unigenes with N(50) length of 2,055 bp were assembled. Of them, 1,594 unigenes were significantly up-regulated and 3353 unigenes were markedly down-regulated under UV-B radiation. These differentially expressed genes (DEGs) involved in UV-B signaling, flavonoid biosynthesis, ROS scavenging, and DNA repair. In addition, a total of 531 metabolites were detected, while flavonoids and anthocyanins accounted for 10.36% of the total compounds. There were 49 upregulated metabolites and 41 downregulated metabolites under UV-B radiation. Flavonoids were the most significantly changed metabolites. qPCR analysis showed that UVR8-COP1-HY5 signaling pathway genes and photolyase genes (i.e., LpUVR3, LpPHR1, and LpDPL) were significantly up-regulated under UV-B light. In addition, the expression levels of JA signaling pathway-related genes (i.e., OPR and JAZ) and flavonoid biosynthesis-related genes were also significantly increased under UV-B radiation. The integrative data analysis showed that UVR8-mediated signaling, jasmonate signaling, flavonoid biosynthesis pathway and DNA repair system might contribute to L. pyriforme acclimating to UV-B radiation. Therefore, these findings present a novel knowledge for understanding the adaption of Antarctic moss to polar environments and provide a foundation for assessing the impact of global climate change on Antarctic land plants. |
format |
Text |
author |
Liu, Shenghao Fang, Shuo Liu, Chenlin Zhao, Linlin Cong, Bailin Zhang, Zhaohui |
author_facet |
Liu, Shenghao Fang, Shuo Liu, Chenlin Zhao, Linlin Cong, Bailin Zhang, Zhaohui |
author_sort |
Liu, Shenghao |
title |
Transcriptomics Integrated With Metabolomics Reveal the Effects of Ultraviolet-B Radiation on Flavonoid Biosynthesis in Antarctic Moss |
title_short |
Transcriptomics Integrated With Metabolomics Reveal the Effects of Ultraviolet-B Radiation on Flavonoid Biosynthesis in Antarctic Moss |
title_full |
Transcriptomics Integrated With Metabolomics Reveal the Effects of Ultraviolet-B Radiation on Flavonoid Biosynthesis in Antarctic Moss |
title_fullStr |
Transcriptomics Integrated With Metabolomics Reveal the Effects of Ultraviolet-B Radiation on Flavonoid Biosynthesis in Antarctic Moss |
title_full_unstemmed |
Transcriptomics Integrated With Metabolomics Reveal the Effects of Ultraviolet-B Radiation on Flavonoid Biosynthesis in Antarctic Moss |
title_sort |
transcriptomics integrated with metabolomics reveal the effects of ultraviolet-b radiation on flavonoid biosynthesis in antarctic moss |
publisher |
Frontiers Media S.A. |
publishDate |
2021 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8692278/ https://doi.org/10.3389/fpls.2021.788377 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Front Plant Sci |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8692278/ http://dx.doi.org/10.3389/fpls.2021.788377 |
op_rights |
Copyright © 2021 Liu, Fang, Liu, Zhao, Cong and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fpls.2021.788377 |
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Frontiers in Plant Science |
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12 |
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1766017821555294208 |