Amino‐functionalised mesoporous silica microspheres for immobilisation of Candida antarctica lipase B – application towards greener production of 2,5‐furandicarboxylic acid
In the present study, amino‐functionalised mesoporous silica microspheres were utilised as support for the covalent immobilisation of Candida antarctica lipase B (CaLB) for the subsequent production of 2,5‐furandicarboxylic acid (FDCA) from 2,5‐diformylfuran (DFF). Under the optimised operating cond...
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ftpubmed:oai:pubmedcentral.nih.gov:8676318 2023-05-15T13:44:10+02:00 Amino‐functionalised mesoporous silica microspheres for immobilisation of Candida antarctica lipase B – application towards greener production of 2,5‐furandicarboxylic acid Saikia, Kongkona Senthil Kumar, Ponnusamy Karanam Rathankumar, Abiram SaiLavanyaa, Sundar Srinivasan, Lakshmi Subramanian, Sivanesan Cabana, Hubert Gosselin, Mathilde Vinoth Kumar, Vaidyanathan 2020-09-29 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8676318/ http://www.ncbi.nlm.nih.gov/pubmed/33108331 https://doi.org/10.1049/iet-nbt.2020.0021 en eng The Institution of Engineering and Technology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8676318/ http://www.ncbi.nlm.nih.gov/pubmed/33108331 http://dx.doi.org/10.1049/iet-nbt.2020.0021 © The Institution of Engineering and Technology IET Nanobiotechnol Research Article Text 2020 ftpubmed https://doi.org/10.1049/iet-nbt.2020.0021 2022-02-06T01:40:50Z In the present study, amino‐functionalised mesoporous silica microspheres were utilised as support for the covalent immobilisation of Candida antarctica lipase B (CaLB) for the subsequent production of 2,5‐furandicarboxylic acid (FDCA) from 2,5‐diformylfuran (DFF). Under the optimised operating conditions of pH 6.5, particle/enzyme ratio of 1.25:1.0 and glutaraldehyde concentration of 4 mM, a maximum CaLB immobilisation yield of 82.4% on silica microspheres was obtained in 12.25 h. The immobilised CaLB was used for the synthesis of alkyl esters, which were utilised along with hydrogen peroxide for FDCA synthesis. The biocatalytic conversion of 30 mM DFF dictated a 77–79% FDCA in 48 h at 30°C; where the turnover number and turnover frequency of immobilised CaLB were 6220.73 mol mol(−1) and 129.59 h(−1), respectively, for ethyl acetate, against 6297.65 mol mol(−1) and 131.2 h(−1), respectively, for ethyl butyrate. Upon examining the operational stability, the immobilised CaLB exhibited high stability till five cycles of FDCA production. Text Antarc* Antarctica PubMed Central (PMC) IET Nanobiotechnology 14 8 732 738 |
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Research Article Saikia, Kongkona Senthil Kumar, Ponnusamy Karanam Rathankumar, Abiram SaiLavanyaa, Sundar Srinivasan, Lakshmi Subramanian, Sivanesan Cabana, Hubert Gosselin, Mathilde Vinoth Kumar, Vaidyanathan Amino‐functionalised mesoporous silica microspheres for immobilisation of Candida antarctica lipase B – application towards greener production of 2,5‐furandicarboxylic acid |
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Research Article |
description |
In the present study, amino‐functionalised mesoporous silica microspheres were utilised as support for the covalent immobilisation of Candida antarctica lipase B (CaLB) for the subsequent production of 2,5‐furandicarboxylic acid (FDCA) from 2,5‐diformylfuran (DFF). Under the optimised operating conditions of pH 6.5, particle/enzyme ratio of 1.25:1.0 and glutaraldehyde concentration of 4 mM, a maximum CaLB immobilisation yield of 82.4% on silica microspheres was obtained in 12.25 h. The immobilised CaLB was used for the synthesis of alkyl esters, which were utilised along with hydrogen peroxide for FDCA synthesis. The biocatalytic conversion of 30 mM DFF dictated a 77–79% FDCA in 48 h at 30°C; where the turnover number and turnover frequency of immobilised CaLB were 6220.73 mol mol(−1) and 129.59 h(−1), respectively, for ethyl acetate, against 6297.65 mol mol(−1) and 131.2 h(−1), respectively, for ethyl butyrate. Upon examining the operational stability, the immobilised CaLB exhibited high stability till five cycles of FDCA production. |
format |
Text |
author |
Saikia, Kongkona Senthil Kumar, Ponnusamy Karanam Rathankumar, Abiram SaiLavanyaa, Sundar Srinivasan, Lakshmi Subramanian, Sivanesan Cabana, Hubert Gosselin, Mathilde Vinoth Kumar, Vaidyanathan |
author_facet |
Saikia, Kongkona Senthil Kumar, Ponnusamy Karanam Rathankumar, Abiram SaiLavanyaa, Sundar Srinivasan, Lakshmi Subramanian, Sivanesan Cabana, Hubert Gosselin, Mathilde Vinoth Kumar, Vaidyanathan |
author_sort |
Saikia, Kongkona |
title |
Amino‐functionalised mesoporous silica microspheres for immobilisation of Candida antarctica lipase B – application towards greener production of 2,5‐furandicarboxylic acid |
title_short |
Amino‐functionalised mesoporous silica microspheres for immobilisation of Candida antarctica lipase B – application towards greener production of 2,5‐furandicarboxylic acid |
title_full |
Amino‐functionalised mesoporous silica microspheres for immobilisation of Candida antarctica lipase B – application towards greener production of 2,5‐furandicarboxylic acid |
title_fullStr |
Amino‐functionalised mesoporous silica microspheres for immobilisation of Candida antarctica lipase B – application towards greener production of 2,5‐furandicarboxylic acid |
title_full_unstemmed |
Amino‐functionalised mesoporous silica microspheres for immobilisation of Candida antarctica lipase B – application towards greener production of 2,5‐furandicarboxylic acid |
title_sort |
amino‐functionalised mesoporous silica microspheres for immobilisation of candida antarctica lipase b – application towards greener production of 2,5‐furandicarboxylic acid |
publisher |
The Institution of Engineering and Technology |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8676318/ http://www.ncbi.nlm.nih.gov/pubmed/33108331 https://doi.org/10.1049/iet-nbt.2020.0021 |
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Antarc* Antarctica |
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Antarc* Antarctica |
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IET Nanobiotechnol |
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8676318/ http://www.ncbi.nlm.nih.gov/pubmed/33108331 http://dx.doi.org/10.1049/iet-nbt.2020.0021 |
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© The Institution of Engineering and Technology |
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https://doi.org/10.1049/iet-nbt.2020.0021 |
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