Expression of transport proteins in the rete mirabile of european silver and yellow eel
BACKGROUND: In physoclist fishes filling of the swimbladder requires acid secretion of gas gland cells to switch on the Root effect and subsequent countercurrent concentration of the initial gas partial pressure increase by back-diffusion of gas molecules in the rete mirabile. It is generally assume...
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ftpubmed:oai:pubmedcentral.nih.gov:8638102 2023-05-15T16:08:42+02:00 Expression of transport proteins in the rete mirabile of european silver and yellow eel Schneebauer, Gabriel Drechsel, Victoria Dirks, Ron Faserl, Klaus Sarg, Bettina Pelster, Bernd 2021-12-02 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8638102/ https://doi.org/10.1186/s12864-021-08180-2 en eng BioMed Central http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8638102/ http://dx.doi.org/10.1186/s12864-021-08180-2 © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. CC0 PDM CC-BY BMC Genomics Research Text 2021 ftpubmed https://doi.org/10.1186/s12864-021-08180-2 2021-12-05T02:09:21Z BACKGROUND: In physoclist fishes filling of the swimbladder requires acid secretion of gas gland cells to switch on the Root effect and subsequent countercurrent concentration of the initial gas partial pressure increase by back-diffusion of gas molecules in the rete mirabile. It is generally assumed that the rete mirabile functions as a passive exchanger, but a detailed analysis of lactate and water movements in the rete mirabile of the eel revealed that lactate is diffusing back in the rete. In the present study we therefore test the hypothesis that expression of transport proteins in rete capillaries allows for back-diffusion of ions and metabolites, which would support the countercurrent concentrating capacity of the rete mirabile. It is also assumed that in silver eels, the migratory stage of the eel, the expression of transport proteins would be enhanced. RESULTS: Analysis of the transcriptome and of the proteome of rete mirabile tissue of the European eel revealed the expression of a large number of membrane ion and metabolite transport proteins, including monocarboxylate and glucose transport proteins. In addition, ion channel proteins, Ca(2+)-ATPase, Na(+)/K(+)-ATPase and also F(1)F(0)-ATP synthase were detected. In contrast to our expectation in silver eels the expression of these transport proteins was not elevated as compared to yellow eels. A remarkable number of enzymes degrading reactive oxygen species (ROS) was detected in rete capillaries. CONCLUSIONS: Our results reveal the expression of a large number of transport proteins in rete capillaries, so that the back diffusion of ions and metabolites, in particular lactate, may significantly enhance the countercurrent concentrating ability of the rete. Metabolic pathways allowing for aerobic generation of ATP supporting secondary active transport mechanisms are established. Rete tissue appears to be equipped with a high ROS defense capacity, preventing damage of the tissue due to the high oxygen partial pressures generated in the countercurrent ... Text European eel PubMed Central (PMC) BMC Genomics 22 1 |
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Research Schneebauer, Gabriel Drechsel, Victoria Dirks, Ron Faserl, Klaus Sarg, Bettina Pelster, Bernd Expression of transport proteins in the rete mirabile of european silver and yellow eel |
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Research |
description |
BACKGROUND: In physoclist fishes filling of the swimbladder requires acid secretion of gas gland cells to switch on the Root effect and subsequent countercurrent concentration of the initial gas partial pressure increase by back-diffusion of gas molecules in the rete mirabile. It is generally assumed that the rete mirabile functions as a passive exchanger, but a detailed analysis of lactate and water movements in the rete mirabile of the eel revealed that lactate is diffusing back in the rete. In the present study we therefore test the hypothesis that expression of transport proteins in rete capillaries allows for back-diffusion of ions and metabolites, which would support the countercurrent concentrating capacity of the rete mirabile. It is also assumed that in silver eels, the migratory stage of the eel, the expression of transport proteins would be enhanced. RESULTS: Analysis of the transcriptome and of the proteome of rete mirabile tissue of the European eel revealed the expression of a large number of membrane ion and metabolite transport proteins, including monocarboxylate and glucose transport proteins. In addition, ion channel proteins, Ca(2+)-ATPase, Na(+)/K(+)-ATPase and also F(1)F(0)-ATP synthase were detected. In contrast to our expectation in silver eels the expression of these transport proteins was not elevated as compared to yellow eels. A remarkable number of enzymes degrading reactive oxygen species (ROS) was detected in rete capillaries. CONCLUSIONS: Our results reveal the expression of a large number of transport proteins in rete capillaries, so that the back diffusion of ions and metabolites, in particular lactate, may significantly enhance the countercurrent concentrating ability of the rete. Metabolic pathways allowing for aerobic generation of ATP supporting secondary active transport mechanisms are established. Rete tissue appears to be equipped with a high ROS defense capacity, preventing damage of the tissue due to the high oxygen partial pressures generated in the countercurrent ... |
format |
Text |
author |
Schneebauer, Gabriel Drechsel, Victoria Dirks, Ron Faserl, Klaus Sarg, Bettina Pelster, Bernd |
author_facet |
Schneebauer, Gabriel Drechsel, Victoria Dirks, Ron Faserl, Klaus Sarg, Bettina Pelster, Bernd |
author_sort |
Schneebauer, Gabriel |
title |
Expression of transport proteins in the rete mirabile of european silver and yellow eel |
title_short |
Expression of transport proteins in the rete mirabile of european silver and yellow eel |
title_full |
Expression of transport proteins in the rete mirabile of european silver and yellow eel |
title_fullStr |
Expression of transport proteins in the rete mirabile of european silver and yellow eel |
title_full_unstemmed |
Expression of transport proteins in the rete mirabile of european silver and yellow eel |
title_sort |
expression of transport proteins in the rete mirabile of european silver and yellow eel |
publisher |
BioMed Central |
publishDate |
2021 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8638102/ https://doi.org/10.1186/s12864-021-08180-2 |
genre |
European eel |
genre_facet |
European eel |
op_source |
BMC Genomics |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8638102/ http://dx.doi.org/10.1186/s12864-021-08180-2 |
op_rights |
© The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
op_rightsnorm |
CC0 PDM CC-BY |
op_doi |
https://doi.org/10.1186/s12864-021-08180-2 |
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BMC Genomics |
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22 |
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