Minnesota peat viromes reveal terrestrial and aquatic niche partitioning for local and global viral populations
BACKGROUND: Peatlands are expected to experience sustained yet fluctuating higher temperatures due to climate change, leading to increased microbial activity and greenhouse gas emissions. Despite mounting evidence for viral contributions to these processes in peatlands underlain with permafrost, lit...
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BioMed Central
2021
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8626947/ http://www.ncbi.nlm.nih.gov/pubmed/34836550 https://doi.org/10.1186/s40168-021-01156-0 |
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Research ter Horst, Anneliek M. Santos-Medellín, Christian Sorensen, Jackson W. Zinke, Laura A. Wilson, Rachel M. Johnston, Eric R. Trubl, Gareth G. Pett-Ridge, Jennifer Blazewicz, Steven J. Hanson, Paul J. Chanton, Jeffrey P. Schadt, Christopher W. Kostka, Joel E. Emerson, Joanne B. Minnesota peat viromes reveal terrestrial and aquatic niche partitioning for local and global viral populations |
topic_facet |
Research |
description |
BACKGROUND: Peatlands are expected to experience sustained yet fluctuating higher temperatures due to climate change, leading to increased microbial activity and greenhouse gas emissions. Despite mounting evidence for viral contributions to these processes in peatlands underlain with permafrost, little is known about viruses in other peatlands. More generally, soil viral biogeography and its potential drivers are poorly understood at both local and global scales. Here, 87 metagenomes and five viral size-fraction metagenomes (viromes) from a boreal peatland in northern Minnesota (the SPRUCE whole-ecosystem warming experiment and surrounding bog) were analyzed for dsDNA viral community ecological patterns, and the recovered viral populations (vOTUs) were compared with our curated PIGEON database of 266,125 vOTUs from diverse ecosystems. RESULTS: Within the SPRUCE experiment, viral community composition was significantly correlated with peat depth, water content, and carbon chemistry, including CH(4) and CO(2) concentrations, but not with temperature during the first 2 years of warming treatments. Peat vOTUs with aquatic-like signatures (shared predicted protein content with marine and/or freshwater vOTUs) were significantly enriched in more waterlogged surface peat depths. Predicted host ranges for SPRUCE vOTUs were relatively narrow, generally within a single bacterial genus. Of the 4326 SPRUCE vOTUs, 164 were previously detected in other soils, mostly peatlands. None of the previously identified 202,371 marine and freshwater vOTUs in our PIGEON database were detected in SPRUCE peat, but 0.4% of 80,714 viral clusters (VCs, grouped by predicted protein content) were shared between soil and aquatic environments. On a per-sample basis, vOTU recovery was 32 times higher from viromes compared with total metagenomes. CONCLUSIONS: Results suggest strong viral “species” boundaries between terrestrial and aquatic ecosystems and to some extent between peat and other soils, with differences less pronounced at higher ... |
format |
Text |
author |
ter Horst, Anneliek M. Santos-Medellín, Christian Sorensen, Jackson W. Zinke, Laura A. Wilson, Rachel M. Johnston, Eric R. Trubl, Gareth G. Pett-Ridge, Jennifer Blazewicz, Steven J. Hanson, Paul J. Chanton, Jeffrey P. Schadt, Christopher W. Kostka, Joel E. Emerson, Joanne B. |
author_facet |
ter Horst, Anneliek M. Santos-Medellín, Christian Sorensen, Jackson W. Zinke, Laura A. Wilson, Rachel M. Johnston, Eric R. Trubl, Gareth G. Pett-Ridge, Jennifer Blazewicz, Steven J. Hanson, Paul J. Chanton, Jeffrey P. Schadt, Christopher W. Kostka, Joel E. Emerson, Joanne B. |
author_sort |
ter Horst, Anneliek M. |
title |
Minnesota peat viromes reveal terrestrial and aquatic niche partitioning for local and global viral populations |
title_short |
Minnesota peat viromes reveal terrestrial and aquatic niche partitioning for local and global viral populations |
title_full |
Minnesota peat viromes reveal terrestrial and aquatic niche partitioning for local and global viral populations |
title_fullStr |
Minnesota peat viromes reveal terrestrial and aquatic niche partitioning for local and global viral populations |
title_full_unstemmed |
Minnesota peat viromes reveal terrestrial and aquatic niche partitioning for local and global viral populations |
title_sort |
minnesota peat viromes reveal terrestrial and aquatic niche partitioning for local and global viral populations |
publisher |
BioMed Central |
publishDate |
2021 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8626947/ http://www.ncbi.nlm.nih.gov/pubmed/34836550 https://doi.org/10.1186/s40168-021-01156-0 |
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permafrost |
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permafrost |
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Microbiome |
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8626947/ http://www.ncbi.nlm.nih.gov/pubmed/34836550 http://dx.doi.org/10.1186/s40168-021-01156-0 |
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© The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
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ftpubmed:oai:pubmedcentral.nih.gov:8626947 2023-05-15T17:58:09+02:00 Minnesota peat viromes reveal terrestrial and aquatic niche partitioning for local and global viral populations ter Horst, Anneliek M. Santos-Medellín, Christian Sorensen, Jackson W. Zinke, Laura A. Wilson, Rachel M. Johnston, Eric R. Trubl, Gareth G. Pett-Ridge, Jennifer Blazewicz, Steven J. Hanson, Paul J. Chanton, Jeffrey P. Schadt, Christopher W. Kostka, Joel E. Emerson, Joanne B. 2021-11-26 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8626947/ http://www.ncbi.nlm.nih.gov/pubmed/34836550 https://doi.org/10.1186/s40168-021-01156-0 en eng BioMed Central http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8626947/ http://www.ncbi.nlm.nih.gov/pubmed/34836550 http://dx.doi.org/10.1186/s40168-021-01156-0 © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. CC0 PDM CC-BY Microbiome Research Text 2021 ftpubmed https://doi.org/10.1186/s40168-021-01156-0 2021-12-05T01:52:45Z BACKGROUND: Peatlands are expected to experience sustained yet fluctuating higher temperatures due to climate change, leading to increased microbial activity and greenhouse gas emissions. Despite mounting evidence for viral contributions to these processes in peatlands underlain with permafrost, little is known about viruses in other peatlands. More generally, soil viral biogeography and its potential drivers are poorly understood at both local and global scales. Here, 87 metagenomes and five viral size-fraction metagenomes (viromes) from a boreal peatland in northern Minnesota (the SPRUCE whole-ecosystem warming experiment and surrounding bog) were analyzed for dsDNA viral community ecological patterns, and the recovered viral populations (vOTUs) were compared with our curated PIGEON database of 266,125 vOTUs from diverse ecosystems. RESULTS: Within the SPRUCE experiment, viral community composition was significantly correlated with peat depth, water content, and carbon chemistry, including CH(4) and CO(2) concentrations, but not with temperature during the first 2 years of warming treatments. Peat vOTUs with aquatic-like signatures (shared predicted protein content with marine and/or freshwater vOTUs) were significantly enriched in more waterlogged surface peat depths. Predicted host ranges for SPRUCE vOTUs were relatively narrow, generally within a single bacterial genus. Of the 4326 SPRUCE vOTUs, 164 were previously detected in other soils, mostly peatlands. None of the previously identified 202,371 marine and freshwater vOTUs in our PIGEON database were detected in SPRUCE peat, but 0.4% of 80,714 viral clusters (VCs, grouped by predicted protein content) were shared between soil and aquatic environments. On a per-sample basis, vOTU recovery was 32 times higher from viromes compared with total metagenomes. CONCLUSIONS: Results suggest strong viral “species” boundaries between terrestrial and aquatic ecosystems and to some extent between peat and other soils, with differences less pronounced at higher ... Text permafrost PubMed Central (PMC) Microbiome 9 1 |