Variable heat shock response in Antarctic biofouling serpulid worms
The classical heat shock response (HSR) with up-regulation of hsp70 in response to warming is often absent in Antarctic marine species. Whilst in Antarctic fish, this is due to a mutation in the gene promoter region resulting in permanent constitutive expression of the inducible form of hsp70; there...
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ftpubmed:oai:pubmedcentral.nih.gov:8578209 2023-05-15T13:38:11+02:00 Variable heat shock response in Antarctic biofouling serpulid worms Nieva, Leyre Villota Peck, Lloyd S. Clark, Melody S. 2021-10-02 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8578209/ http://www.ncbi.nlm.nih.gov/pubmed/34601709 https://doi.org/10.1007/s12192-021-01235-z en eng Springer Netherlands http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8578209/ http://www.ncbi.nlm.nih.gov/pubmed/34601709 http://dx.doi.org/10.1007/s12192-021-01235-z © Cell Stress Society International 2021 Cell Stress Chaperones Original Paper Text 2021 ftpubmed https://doi.org/10.1007/s12192-021-01235-z 2022-05-08T00:36:19Z The classical heat shock response (HSR) with up-regulation of hsp70 in response to warming is often absent in Antarctic marine species. Whilst in Antarctic fish, this is due to a mutation in the gene promoter region resulting in permanent constitutive expression of the inducible form of hsp70; there are further questions as to whether evolution to life below 0 °C has resulted in a generalised alteration to the HSR in Antarctic marine invertebrates. However, the number of species investigated to date is limited. In the first evaluation of the HSR in two spirorbid polychaetes Romanchella perrieri and Protolaeospira stalagmia, we show highly variable results of HSR induction depending on warming regimes. These animals were subjected to in situ warming (+ 1 °C and + 2 °C above ambient conditions) using heated settlement panels for 18 months, and then the HSR was tested in R. perrieri using acute and chronic temperature elevation trials. The classic HSR was not induced in response to acute thermal challenge in this species (2 h at 15 °C) and significant down-regulation of hsp90 occurred during chronic warming at 4 °C for 30 days. Analysis of heat shock protein (HSP) genes in a transcriptome study of P. stalagmia, which had been warmed in situ for 18 months, showed up-regulation of HSP70 and HSP90 family members, thus further emphasising the complexity of the response in Antarctic marine species. It is increasingly apparent that the Antarctic HSR has evolved in a species-specific manner to life in the cold. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12192-021-01235-z. Text Antarc* Antarctic PubMed Central (PMC) Antarctic The Antarctic Cell Stress and Chaperones 26 6 945 954 |
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Original Paper Nieva, Leyre Villota Peck, Lloyd S. Clark, Melody S. Variable heat shock response in Antarctic biofouling serpulid worms |
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Original Paper |
description |
The classical heat shock response (HSR) with up-regulation of hsp70 in response to warming is often absent in Antarctic marine species. Whilst in Antarctic fish, this is due to a mutation in the gene promoter region resulting in permanent constitutive expression of the inducible form of hsp70; there are further questions as to whether evolution to life below 0 °C has resulted in a generalised alteration to the HSR in Antarctic marine invertebrates. However, the number of species investigated to date is limited. In the first evaluation of the HSR in two spirorbid polychaetes Romanchella perrieri and Protolaeospira stalagmia, we show highly variable results of HSR induction depending on warming regimes. These animals were subjected to in situ warming (+ 1 °C and + 2 °C above ambient conditions) using heated settlement panels for 18 months, and then the HSR was tested in R. perrieri using acute and chronic temperature elevation trials. The classic HSR was not induced in response to acute thermal challenge in this species (2 h at 15 °C) and significant down-regulation of hsp90 occurred during chronic warming at 4 °C for 30 days. Analysis of heat shock protein (HSP) genes in a transcriptome study of P. stalagmia, which had been warmed in situ for 18 months, showed up-regulation of HSP70 and HSP90 family members, thus further emphasising the complexity of the response in Antarctic marine species. It is increasingly apparent that the Antarctic HSR has evolved in a species-specific manner to life in the cold. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12192-021-01235-z. |
format |
Text |
author |
Nieva, Leyre Villota Peck, Lloyd S. Clark, Melody S. |
author_facet |
Nieva, Leyre Villota Peck, Lloyd S. Clark, Melody S. |
author_sort |
Nieva, Leyre Villota |
title |
Variable heat shock response in Antarctic biofouling serpulid worms |
title_short |
Variable heat shock response in Antarctic biofouling serpulid worms |
title_full |
Variable heat shock response in Antarctic biofouling serpulid worms |
title_fullStr |
Variable heat shock response in Antarctic biofouling serpulid worms |
title_full_unstemmed |
Variable heat shock response in Antarctic biofouling serpulid worms |
title_sort |
variable heat shock response in antarctic biofouling serpulid worms |
publisher |
Springer Netherlands |
publishDate |
2021 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8578209/ http://www.ncbi.nlm.nih.gov/pubmed/34601709 https://doi.org/10.1007/s12192-021-01235-z |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Cell Stress Chaperones |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8578209/ http://www.ncbi.nlm.nih.gov/pubmed/34601709 http://dx.doi.org/10.1007/s12192-021-01235-z |
op_rights |
© Cell Stress Society International 2021 |
op_doi |
https://doi.org/10.1007/s12192-021-01235-z |
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Cell Stress and Chaperones |
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