Morphological and spectroscopic analysis of snow and glacier algae and their parasitic fungi on different glaciers of Svalbard
The results show the morphological analyses and spectroscopic studies of snow and glacier algae and their parasitic fungi in Svalbard (High Arctic). Fixed algal cells of two species, Sanguina nivaloides and Ancylonema nordenskioeldii, were imaged using light microscopy, scanning electron microscopy...
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ftpubmed:oai:pubmedcentral.nih.gov:8575968 2023-05-15T15:05:58+02:00 Morphological and spectroscopic analysis of snow and glacier algae and their parasitic fungi on different glaciers of Svalbard Fiołka, Marta J. Takeuchi, Nozomu Sofińska-Chmiel, Weronika Wójcik-Mieszawska, Sylwia Irvine-Fynn, Tristram Edwards, Arwyn 2021-11-08 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8575968/ http://www.ncbi.nlm.nih.gov/pubmed/34750421 https://doi.org/10.1038/s41598-021-01211-8 en eng Nature Publishing Group UK http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8575968/ http://www.ncbi.nlm.nih.gov/pubmed/34750421 http://dx.doi.org/10.1038/s41598-021-01211-8 © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . CC-BY Sci Rep Article Text 2021 ftpubmed https://doi.org/10.1038/s41598-021-01211-8 2021-11-14T01:46:59Z The results show the morphological analyses and spectroscopic studies of snow and glacier algae and their parasitic fungi in Svalbard (High Arctic). Fixed algal cells of two species, Sanguina nivaloides and Ancylonema nordenskioeldii, were imaged using light microscopy, scanning electron microscopy (SEM), and atomic force microscopy (AFM). Fluorescence microscopy using Calcofluor white stain supported the observations of parasitic fungi on the algal cells. Images in brightfield microscopy showed chytrid-like fungi penetrating the cells of both algal species. Parasites were found to colonize the cells of A. nordenskioeldii and hypnozygotes of S. nivaloides, while no fungi infected the cyst stages of S. nivaloides. The autofluorescence analysis revealed the ability of S. nivaloides to glow when excited with different wavelengths, while A. nordenskioeldii did not fluoresce. The hypnozygotes of S. nivaloides emitted brighter fluorescence than the cysts, and the most intense luminosity was observed in the UV range. The Fourier-transform infrared spectroscopy (FTIR) and energy-dispersive X-ray spectroscopy (EDS) spectroscopic analysis showed differences in the chemical composition between samples collected from three different sites. Samples dominated by cyst cells were characterized by the presence of an abundant polysaccharide envelope. Text Arctic glacier Svalbard PubMed Central (PMC) Arctic Svalbard Scientific Reports 11 1 |
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Article Fiołka, Marta J. Takeuchi, Nozomu Sofińska-Chmiel, Weronika Wójcik-Mieszawska, Sylwia Irvine-Fynn, Tristram Edwards, Arwyn Morphological and spectroscopic analysis of snow and glacier algae and their parasitic fungi on different glaciers of Svalbard |
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Article |
description |
The results show the morphological analyses and spectroscopic studies of snow and glacier algae and their parasitic fungi in Svalbard (High Arctic). Fixed algal cells of two species, Sanguina nivaloides and Ancylonema nordenskioeldii, were imaged using light microscopy, scanning electron microscopy (SEM), and atomic force microscopy (AFM). Fluorescence microscopy using Calcofluor white stain supported the observations of parasitic fungi on the algal cells. Images in brightfield microscopy showed chytrid-like fungi penetrating the cells of both algal species. Parasites were found to colonize the cells of A. nordenskioeldii and hypnozygotes of S. nivaloides, while no fungi infected the cyst stages of S. nivaloides. The autofluorescence analysis revealed the ability of S. nivaloides to glow when excited with different wavelengths, while A. nordenskioeldii did not fluoresce. The hypnozygotes of S. nivaloides emitted brighter fluorescence than the cysts, and the most intense luminosity was observed in the UV range. The Fourier-transform infrared spectroscopy (FTIR) and energy-dispersive X-ray spectroscopy (EDS) spectroscopic analysis showed differences in the chemical composition between samples collected from three different sites. Samples dominated by cyst cells were characterized by the presence of an abundant polysaccharide envelope. |
format |
Text |
author |
Fiołka, Marta J. Takeuchi, Nozomu Sofińska-Chmiel, Weronika Wójcik-Mieszawska, Sylwia Irvine-Fynn, Tristram Edwards, Arwyn |
author_facet |
Fiołka, Marta J. Takeuchi, Nozomu Sofińska-Chmiel, Weronika Wójcik-Mieszawska, Sylwia Irvine-Fynn, Tristram Edwards, Arwyn |
author_sort |
Fiołka, Marta J. |
title |
Morphological and spectroscopic analysis of snow and glacier algae and their parasitic fungi on different glaciers of Svalbard |
title_short |
Morphological and spectroscopic analysis of snow and glacier algae and their parasitic fungi on different glaciers of Svalbard |
title_full |
Morphological and spectroscopic analysis of snow and glacier algae and their parasitic fungi on different glaciers of Svalbard |
title_fullStr |
Morphological and spectroscopic analysis of snow and glacier algae and their parasitic fungi on different glaciers of Svalbard |
title_full_unstemmed |
Morphological and spectroscopic analysis of snow and glacier algae and their parasitic fungi on different glaciers of Svalbard |
title_sort |
morphological and spectroscopic analysis of snow and glacier algae and their parasitic fungi on different glaciers of svalbard |
publisher |
Nature Publishing Group UK |
publishDate |
2021 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8575968/ http://www.ncbi.nlm.nih.gov/pubmed/34750421 https://doi.org/10.1038/s41598-021-01211-8 |
geographic |
Arctic Svalbard |
geographic_facet |
Arctic Svalbard |
genre |
Arctic glacier Svalbard |
genre_facet |
Arctic glacier Svalbard |
op_source |
Sci Rep |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8575968/ http://www.ncbi.nlm.nih.gov/pubmed/34750421 http://dx.doi.org/10.1038/s41598-021-01211-8 |
op_rights |
© The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
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CC-BY |
op_doi |
https://doi.org/10.1038/s41598-021-01211-8 |
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Scientific Reports |
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