Lentil and Fava Bean With Contrasting Germination Kinetics: A Focus on Digestion of Proteins and Bioactivity of Resistant Peptides
Germination offers advantages to improve legume protein digestibility as it disintegrates seed structure and hydrolyzes proteins and anti-nutrients. Seed permeability (related to polyphenol content of seed coats) is an important factor affecting the duration of seed germination and its impact on pro...
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8575454/ http://www.ncbi.nlm.nih.gov/pubmed/34759946 https://doi.org/10.3389/fpls.2021.754287 |
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ftpubmed:oai:pubmedcentral.nih.gov:8575454 2023-05-15T15:41:55+02:00 Lentil and Fava Bean With Contrasting Germination Kinetics: A Focus on Digestion of Proteins and Bioactivity of Resistant Peptides Bautista-Expósito, Sara Vandenberg, Albert Peñas, Elena Frias, Juana Martínez-Villaluenga, Cristina 2021-10-25 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8575454/ http://www.ncbi.nlm.nih.gov/pubmed/34759946 https://doi.org/10.3389/fpls.2021.754287 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8575454/ http://www.ncbi.nlm.nih.gov/pubmed/34759946 http://dx.doi.org/10.3389/fpls.2021.754287 Copyright © 2021 Bautista-Expósito, Vandenberg, Peñas, Frias and Martínez-Villaluenga. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. CC-BY Front Plant Sci Plant Science Text 2021 ftpubmed https://doi.org/10.3389/fpls.2021.754287 2021-11-14T01:46:20Z Germination offers advantages to improve legume protein digestibility as it disintegrates seed structure and hydrolyzes proteins and anti-nutrients. Seed permeability (related to polyphenol content of seed coats) is an important factor affecting the duration of seed germination and its impact on protein digestibility and bioactivity. The objective was to compare the effect of seed germination on protease activity, structure, and proteolysis of four selected legumes with contrasting seed coat polyphenol profiles (gray zero-tannin lentil [GZL], beluga lentil [BL], and dehulled red lentil [DL]; and zero tannin/low vicine–convicine fava bean [ZF]). Protein hydrolysis was characterized during germination and digestion with respect to proteins, peptides, and free amino acids (FAAs). In vitro antihypertensive and antioxidant activities of digests were investigated, and the peptidomic characterization [high-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS)] and identification of bioactive fragments in intestinal digests were performed. Regardless of the seed type, germination increased protease activity and reduced the levels of phytic acid, trypsin inhibitors, and tannins (only in BL). A significant proteolysis of the 7S and 11S globulins and a concomitant increase of peptides and FAAs were observed in all sprouted legumes. Digestion kinetics in sprouts revealed a faster generation of FAAs and peptides than in dry seeds, with changes being more evident for DL, associated with a faster imbibition, germination, and sprout growth. In contrast, BL sprouts showed the lowest protein digestibility, likely due to a lower protease activity, seed structure disintegration, and higher anti-nutrient levels in comparison to GZL, DL, and ZF. Moreover, the digestion of sprouts resulted in a higher number of resistant peptides in DL and ZF that matched with previously reported bioactive sequences, suggesting a promising health potential of legume sprouts that was confirmed in vitro. The results ... Text Beluga Beluga* PubMed Central (PMC) Frontiers in Plant Science 12 |
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English |
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Plant Science |
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Plant Science Bautista-Expósito, Sara Vandenberg, Albert Peñas, Elena Frias, Juana Martínez-Villaluenga, Cristina Lentil and Fava Bean With Contrasting Germination Kinetics: A Focus on Digestion of Proteins and Bioactivity of Resistant Peptides |
topic_facet |
Plant Science |
description |
Germination offers advantages to improve legume protein digestibility as it disintegrates seed structure and hydrolyzes proteins and anti-nutrients. Seed permeability (related to polyphenol content of seed coats) is an important factor affecting the duration of seed germination and its impact on protein digestibility and bioactivity. The objective was to compare the effect of seed germination on protease activity, structure, and proteolysis of four selected legumes with contrasting seed coat polyphenol profiles (gray zero-tannin lentil [GZL], beluga lentil [BL], and dehulled red lentil [DL]; and zero tannin/low vicine–convicine fava bean [ZF]). Protein hydrolysis was characterized during germination and digestion with respect to proteins, peptides, and free amino acids (FAAs). In vitro antihypertensive and antioxidant activities of digests were investigated, and the peptidomic characterization [high-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS)] and identification of bioactive fragments in intestinal digests were performed. Regardless of the seed type, germination increased protease activity and reduced the levels of phytic acid, trypsin inhibitors, and tannins (only in BL). A significant proteolysis of the 7S and 11S globulins and a concomitant increase of peptides and FAAs were observed in all sprouted legumes. Digestion kinetics in sprouts revealed a faster generation of FAAs and peptides than in dry seeds, with changes being more evident for DL, associated with a faster imbibition, germination, and sprout growth. In contrast, BL sprouts showed the lowest protein digestibility, likely due to a lower protease activity, seed structure disintegration, and higher anti-nutrient levels in comparison to GZL, DL, and ZF. Moreover, the digestion of sprouts resulted in a higher number of resistant peptides in DL and ZF that matched with previously reported bioactive sequences, suggesting a promising health potential of legume sprouts that was confirmed in vitro. The results ... |
format |
Text |
author |
Bautista-Expósito, Sara Vandenberg, Albert Peñas, Elena Frias, Juana Martínez-Villaluenga, Cristina |
author_facet |
Bautista-Expósito, Sara Vandenberg, Albert Peñas, Elena Frias, Juana Martínez-Villaluenga, Cristina |
author_sort |
Bautista-Expósito, Sara |
title |
Lentil and Fava Bean With Contrasting Germination Kinetics: A Focus on Digestion of Proteins and Bioactivity of Resistant Peptides |
title_short |
Lentil and Fava Bean With Contrasting Germination Kinetics: A Focus on Digestion of Proteins and Bioactivity of Resistant Peptides |
title_full |
Lentil and Fava Bean With Contrasting Germination Kinetics: A Focus on Digestion of Proteins and Bioactivity of Resistant Peptides |
title_fullStr |
Lentil and Fava Bean With Contrasting Germination Kinetics: A Focus on Digestion of Proteins and Bioactivity of Resistant Peptides |
title_full_unstemmed |
Lentil and Fava Bean With Contrasting Germination Kinetics: A Focus on Digestion of Proteins and Bioactivity of Resistant Peptides |
title_sort |
lentil and fava bean with contrasting germination kinetics: a focus on digestion of proteins and bioactivity of resistant peptides |
publisher |
Frontiers Media S.A. |
publishDate |
2021 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8575454/ http://www.ncbi.nlm.nih.gov/pubmed/34759946 https://doi.org/10.3389/fpls.2021.754287 |
genre |
Beluga Beluga* |
genre_facet |
Beluga Beluga* |
op_source |
Front Plant Sci |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8575454/ http://www.ncbi.nlm.nih.gov/pubmed/34759946 http://dx.doi.org/10.3389/fpls.2021.754287 |
op_rights |
Copyright © 2021 Bautista-Expósito, Vandenberg, Peñas, Frias and Martínez-Villaluenga. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fpls.2021.754287 |
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Frontiers in Plant Science |
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12 |
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1766374799690432512 |