Whole genome sequencing and comparative genomic analyses of Planococcus alpniumensis MSAK28401(T), a new species isolated from Antarctic krill

• Published in: BMC Microbiology
• Main Authors: Wang, Yuanyuan, Ma, Lingbo, He, Jian, Liu, Zixuan, Weng, Shaoping, Wang, Lumin, He, Jianguo, Guo, Changjun
• Format: Text
• Language: English
• Published: BioMed Central 2021
• Subjects: Research; Antarctic; Antarc*; Antarctic Krill
• Online Access: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8532331/; http://www.ncbi.nlm.nih.gov/pubmed/34686131; https://doi.org/10.1186/s12866-021-02347-3

BACKGROUND: Extremophiles have attracted much attention in the last few decades, as they possess different properties by producing certain useful metabolites. However, the secondary metabolism of the extremophiles of Antarctic krill has received little attention. RESULTS: In this study, a new bacterial strain MSAK28401(T) from Antarctic krill was isolated and identified. The results of analysis on phenotypic, chemotaxonomic, and genomic characteristics showed that the strain MSAK28401(T) belongs to the genus Planococcus. Cells of this strain were coccoid (0.89–1.05 μm) and aerobic. The majority of the fatty acid content was C(15:0) anteiso (37.67 ± 0.90%) followed by C(16:1) ω7c alcohol (10.37 ± 1.22%) and C(16:0) iso (9.36 ± 0.71%). The calculated average nucleotide identity and DNA–DNA hybridization values between the strain MSAK28401(T) and type strains P. citreus DSM 20549(T) and P. rifietoensis M8(T) were lower than 91 and 70%, respectively. The strain MSAK28401(T) (=KCTC 43283(T) and MCCC 1k05448(T)) represented a new member of the genus Planococcus and was named P. alpniumensis sp. nov. Moreover, genes involved in the degradation of aromatic compounds (e.g., salicylate, gentisate, and quinate) were found in the genome, implying that strain MSAK28401(T) has an aromatic compound as its potential metabolite. This work will help us understand the genomic characteristics and potential metabolic pathway of Planococcus from Antarctic krill. CONCLUSIONS: This study reported the genomic information and phenotypic characteristics of the new strain P. alpniumensis MSAK28401(T) isolated from Antarctic krill, and provided the genome information of Planococcus strains for further studying the function roles in aromatic compound metabolism. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-021-02347-3.