Morphological and molecular identification of Cryptocotyle lingua metacercariae isolated from Atlantic cod (Gadus morhua) from Danish seas and whiting (Merlangius merlangus) from the English Channel
Trematode larvae (metacercariae) causing black spot disease occur frequently in gills, fins, skin and the superficial muscle layers of marine fish. Species within the genus Cryptocotyle Lühe, 1899 are frequently associated with this disease. Descriptions of the metacercarial stage are relatively lim...
Published in: | Parasitology Research |
---|---|
Main Authors: | , , , , |
Format: | Text |
Language: | English |
Published: |
Springer Berlin Heidelberg
2021
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8460567/ http://www.ncbi.nlm.nih.gov/pubmed/34448921 https://doi.org/10.1007/s00436-021-07278-6 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:8460567 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:8460567 2023-05-15T15:27:09+02:00 Morphological and molecular identification of Cryptocotyle lingua metacercariae isolated from Atlantic cod (Gadus morhua) from Danish seas and whiting (Merlangius merlangus) from the English Channel Duflot, Maureen Gay, Mélanie Midelet, Graziella Kania, Per Walter Buchmann, Kurt 2021-08-27 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8460567/ http://www.ncbi.nlm.nih.gov/pubmed/34448921 https://doi.org/10.1007/s00436-021-07278-6 en eng Springer Berlin Heidelberg http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8460567/ http://www.ncbi.nlm.nih.gov/pubmed/34448921 http://dx.doi.org/10.1007/s00436-021-07278-6 © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . CC-BY Parasitol Res Fish Parasitology - Original Paper Text 2021 ftpubmed https://doi.org/10.1007/s00436-021-07278-6 2021-10-10T00:27:13Z Trematode larvae (metacercariae) causing black spot disease occur frequently in gills, fins, skin and the superficial muscle layers of marine fish. Species within the genus Cryptocotyle Lühe, 1899 are frequently associated with this disease. Descriptions of the metacercarial stage are relatively limited and none has hitherto been reported from fish from the English Channel. The present study reports the morphological and molecular identifications of encysted black spot-inducing parasites from whiting (Merlangius merlangus) and Atlantic cod (Gadus morhua) caught respectively from the north coast of France (English Channel) and from Danish sea waters. Metacercariae were characterised morphologically based on microscopic observations and molecularly using Sanger sequencing of fragments of the mitochondrial cox1 gene and rDNA ITS region. Morphological data were compared with available data in the literature. Phylogenetic trees including reference sequences were built to confirm morphological and molecular identifications. This survey constitutes the first description of C. lingua metacercariae in the English Channel ecosystems. Text atlantic cod Gadus morhua PubMed Central (PMC) Parasitology Research 120 10 3417 3427 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Fish Parasitology - Original Paper |
spellingShingle |
Fish Parasitology - Original Paper Duflot, Maureen Gay, Mélanie Midelet, Graziella Kania, Per Walter Buchmann, Kurt Morphological and molecular identification of Cryptocotyle lingua metacercariae isolated from Atlantic cod (Gadus morhua) from Danish seas and whiting (Merlangius merlangus) from the English Channel |
topic_facet |
Fish Parasitology - Original Paper |
description |
Trematode larvae (metacercariae) causing black spot disease occur frequently in gills, fins, skin and the superficial muscle layers of marine fish. Species within the genus Cryptocotyle Lühe, 1899 are frequently associated with this disease. Descriptions of the metacercarial stage are relatively limited and none has hitherto been reported from fish from the English Channel. The present study reports the morphological and molecular identifications of encysted black spot-inducing parasites from whiting (Merlangius merlangus) and Atlantic cod (Gadus morhua) caught respectively from the north coast of France (English Channel) and from Danish sea waters. Metacercariae were characterised morphologically based on microscopic observations and molecularly using Sanger sequencing of fragments of the mitochondrial cox1 gene and rDNA ITS region. Morphological data were compared with available data in the literature. Phylogenetic trees including reference sequences were built to confirm morphological and molecular identifications. This survey constitutes the first description of C. lingua metacercariae in the English Channel ecosystems. |
format |
Text |
author |
Duflot, Maureen Gay, Mélanie Midelet, Graziella Kania, Per Walter Buchmann, Kurt |
author_facet |
Duflot, Maureen Gay, Mélanie Midelet, Graziella Kania, Per Walter Buchmann, Kurt |
author_sort |
Duflot, Maureen |
title |
Morphological and molecular identification of Cryptocotyle lingua metacercariae isolated from Atlantic cod (Gadus morhua) from Danish seas and whiting (Merlangius merlangus) from the English Channel |
title_short |
Morphological and molecular identification of Cryptocotyle lingua metacercariae isolated from Atlantic cod (Gadus morhua) from Danish seas and whiting (Merlangius merlangus) from the English Channel |
title_full |
Morphological and molecular identification of Cryptocotyle lingua metacercariae isolated from Atlantic cod (Gadus morhua) from Danish seas and whiting (Merlangius merlangus) from the English Channel |
title_fullStr |
Morphological and molecular identification of Cryptocotyle lingua metacercariae isolated from Atlantic cod (Gadus morhua) from Danish seas and whiting (Merlangius merlangus) from the English Channel |
title_full_unstemmed |
Morphological and molecular identification of Cryptocotyle lingua metacercariae isolated from Atlantic cod (Gadus morhua) from Danish seas and whiting (Merlangius merlangus) from the English Channel |
title_sort |
morphological and molecular identification of cryptocotyle lingua metacercariae isolated from atlantic cod (gadus morhua) from danish seas and whiting (merlangius merlangus) from the english channel |
publisher |
Springer Berlin Heidelberg |
publishDate |
2021 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8460567/ http://www.ncbi.nlm.nih.gov/pubmed/34448921 https://doi.org/10.1007/s00436-021-07278-6 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
Parasitol Res |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8460567/ http://www.ncbi.nlm.nih.gov/pubmed/34448921 http://dx.doi.org/10.1007/s00436-021-07278-6 |
op_rights |
© The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1007/s00436-021-07278-6 |
container_title |
Parasitology Research |
container_volume |
120 |
container_issue |
10 |
container_start_page |
3417 |
op_container_end_page |
3427 |
_version_ |
1766357601184907264 |