Metagenomic strategies identify diverse integron‐integrase and antibiotic resistance genes in the Antarctic environment
The objective of this study is to identify and analyze integrons and antibiotic resistance genes (ARGs) in samples collected from diverse sites in terrestrial Antarctica. Integrons were studied using two independent methods. One involved the construction and analysis of intI gene amplicon libraries....
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ftpubmed:oai:pubmedcentral.nih.gov:8435808 2023-05-15T14:02:24+02:00 Metagenomic strategies identify diverse integron‐integrase and antibiotic resistance genes in the Antarctic environment Antelo, Verónica Giménez, Matías Azziz, Gastón Valdespino‐Castillo, Patricia Falcón, Luisa I. Ruberto, Lucas A. M. Mac Cormack, Walter P. Mazel, Didier Batista, Silvia 2021-09-12 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8435808/ https://doi.org/10.1002/mbo3.1219 en eng John Wiley and Sons Inc. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8435808/ http://dx.doi.org/10.1002/mbo3.1219 © 2021 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. CC-BY-NC Microbiologyopen Original Articles Text 2021 ftpubmed https://doi.org/10.1002/mbo3.1219 2021-09-19T00:41:08Z The objective of this study is to identify and analyze integrons and antibiotic resistance genes (ARGs) in samples collected from diverse sites in terrestrial Antarctica. Integrons were studied using two independent methods. One involved the construction and analysis of intI gene amplicon libraries. In addition, we sequenced 17 metagenomes of microbial mats and soil by high‐throughput sequencing and analyzed these data using the IntegronFinder program. As expected, the metagenomic analysis allowed for the identification of novel predicted intI integrases and gene cassettes (GCs), which mostly encode unknown functions. However, some intI genes are similar to sequences previously identified by amplicon library analysis in soil samples collected from non‐Antarctic sites. ARGs were analyzed in the metagenomes using ABRIcate with CARD database and verified if these genes could be classified as GCs by IntegronFinder. We identified 53 ARGs in 15 metagenomes, but only four were classified as GCs, one in MTG12 metagenome (Continental Antarctica), encoding an aminoglycoside‐modifying enzyme (AAC(6´)acetyltransferase) and the other three in CS1 metagenome (Maritime Antarctica). One of these genes encodes a class D β‐lactamase (blaOXA‐205) and the other two are located in the same contig. One is part of a gene encoding the first 76 amino acids of aminoglycoside adenyltransferase (aadA6), and the other is a qacG2 gene. Text Antarc* Antarctic Antarctica PubMed Central (PMC) Antarctic The Antarctic MicrobiologyOpen 10 5 |
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Original Articles |
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Original Articles Antelo, Verónica Giménez, Matías Azziz, Gastón Valdespino‐Castillo, Patricia Falcón, Luisa I. Ruberto, Lucas A. M. Mac Cormack, Walter P. Mazel, Didier Batista, Silvia Metagenomic strategies identify diverse integron‐integrase and antibiotic resistance genes in the Antarctic environment |
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Original Articles |
description |
The objective of this study is to identify and analyze integrons and antibiotic resistance genes (ARGs) in samples collected from diverse sites in terrestrial Antarctica. Integrons were studied using two independent methods. One involved the construction and analysis of intI gene amplicon libraries. In addition, we sequenced 17 metagenomes of microbial mats and soil by high‐throughput sequencing and analyzed these data using the IntegronFinder program. As expected, the metagenomic analysis allowed for the identification of novel predicted intI integrases and gene cassettes (GCs), which mostly encode unknown functions. However, some intI genes are similar to sequences previously identified by amplicon library analysis in soil samples collected from non‐Antarctic sites. ARGs were analyzed in the metagenomes using ABRIcate with CARD database and verified if these genes could be classified as GCs by IntegronFinder. We identified 53 ARGs in 15 metagenomes, but only four were classified as GCs, one in MTG12 metagenome (Continental Antarctica), encoding an aminoglycoside‐modifying enzyme (AAC(6´)acetyltransferase) and the other three in CS1 metagenome (Maritime Antarctica). One of these genes encodes a class D β‐lactamase (blaOXA‐205) and the other two are located in the same contig. One is part of a gene encoding the first 76 amino acids of aminoglycoside adenyltransferase (aadA6), and the other is a qacG2 gene. |
format |
Text |
author |
Antelo, Verónica Giménez, Matías Azziz, Gastón Valdespino‐Castillo, Patricia Falcón, Luisa I. Ruberto, Lucas A. M. Mac Cormack, Walter P. Mazel, Didier Batista, Silvia |
author_facet |
Antelo, Verónica Giménez, Matías Azziz, Gastón Valdespino‐Castillo, Patricia Falcón, Luisa I. Ruberto, Lucas A. M. Mac Cormack, Walter P. Mazel, Didier Batista, Silvia |
author_sort |
Antelo, Verónica |
title |
Metagenomic strategies identify diverse integron‐integrase and antibiotic resistance genes in the Antarctic environment |
title_short |
Metagenomic strategies identify diverse integron‐integrase and antibiotic resistance genes in the Antarctic environment |
title_full |
Metagenomic strategies identify diverse integron‐integrase and antibiotic resistance genes in the Antarctic environment |
title_fullStr |
Metagenomic strategies identify diverse integron‐integrase and antibiotic resistance genes in the Antarctic environment |
title_full_unstemmed |
Metagenomic strategies identify diverse integron‐integrase and antibiotic resistance genes in the Antarctic environment |
title_sort |
metagenomic strategies identify diverse integron‐integrase and antibiotic resistance genes in the antarctic environment |
publisher |
John Wiley and Sons Inc. |
publishDate |
2021 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8435808/ https://doi.org/10.1002/mbo3.1219 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Microbiologyopen |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8435808/ http://dx.doi.org/10.1002/mbo3.1219 |
op_rights |
© 2021 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
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CC-BY-NC |
op_doi |
https://doi.org/10.1002/mbo3.1219 |
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MicrobiologyOpen |
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10 |
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5 |
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1766272667679195136 |