Chemical Characterization of Atlantic Cod (Gadus morhua) Collagen Hydrolyzed Using Enzyme Preparation Derived from Red King Crab (Paralithodes camtschaticus) and Its Potential as a Core Component of Bacterial Culture Medium
The Atlantic cod (Gadus morhua) and red king crab (Paralithodes camtschaticus) processing wastes are massive and unutilized in the Murmansk region of Russia. The samples of skin-containing waste of Atlantic cod fillets production were hydrolyzed using enzyme preparations derived from red king crab h...
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8399610/ http://www.ncbi.nlm.nih.gov/pubmed/34436311 https://doi.org/10.3390/md19080472 |
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ftpubmed:oai:pubmedcentral.nih.gov:8399610 2023-05-15T15:26:58+02:00 Chemical Characterization of Atlantic Cod (Gadus morhua) Collagen Hydrolyzed Using Enzyme Preparation Derived from Red King Crab (Paralithodes camtschaticus) and Its Potential as a Core Component of Bacterial Culture Medium Novikov, Vitaliy Yu. Shumskaya, Nadezhda V. Mukhin, Vyacheslav A. Zolotarev, Konstantin V. Mikhailov, Anton N. Nakhod, Valeriya I. Mikhailova, Marina V. 2021-08-23 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8399610/ http://www.ncbi.nlm.nih.gov/pubmed/34436311 https://doi.org/10.3390/md19080472 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8399610/ http://www.ncbi.nlm.nih.gov/pubmed/34436311 http://dx.doi.org/10.3390/md19080472 © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). CC-BY Mar Drugs Article Text 2021 ftpubmed https://doi.org/10.3390/md19080472 2021-09-05T00:55:30Z The Atlantic cod (Gadus morhua) and red king crab (Paralithodes camtschaticus) processing wastes are massive and unutilized in the Murmansk region of Russia. The samples of skin-containing waste of Atlantic cod fillets production were hydrolyzed using enzyme preparations derived from red king crab hepatopancreases, porcine pancreases, and Bacillus subtilis bacteria. The activity of enzymes from crab hepatopancreases was significantly higher than the activity of enzymes derived from other sources. The optimal conditions of the hydrolysis process have been figured out. The samples of cod processing waste hydrolysate were analyzed for amino acid composition and molecular weight distribution. The samples of hydrolysate were used as core components for bacterial culture medium samples. The efficiency of the medium samples was tested for Escherichia coli growth rate; the most efficient sample had an efficiency of 95.3% of that of a commercially available medium based on fish meal. Substitution of medium components with those derived from industrial by-products is one of the ways to decrease a cost of a culture medium in biopharmaceutical drug production. Text atlantic cod Gadus morhua Paralithodes camtschaticus Red king crab PubMed Central (PMC) Murmansk Marine Drugs 19 8 472 |
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Article Novikov, Vitaliy Yu. Shumskaya, Nadezhda V. Mukhin, Vyacheslav A. Zolotarev, Konstantin V. Mikhailov, Anton N. Nakhod, Valeriya I. Mikhailova, Marina V. Chemical Characterization of Atlantic Cod (Gadus morhua) Collagen Hydrolyzed Using Enzyme Preparation Derived from Red King Crab (Paralithodes camtschaticus) and Its Potential as a Core Component of Bacterial Culture Medium |
topic_facet |
Article |
description |
The Atlantic cod (Gadus morhua) and red king crab (Paralithodes camtschaticus) processing wastes are massive and unutilized in the Murmansk region of Russia. The samples of skin-containing waste of Atlantic cod fillets production were hydrolyzed using enzyme preparations derived from red king crab hepatopancreases, porcine pancreases, and Bacillus subtilis bacteria. The activity of enzymes from crab hepatopancreases was significantly higher than the activity of enzymes derived from other sources. The optimal conditions of the hydrolysis process have been figured out. The samples of cod processing waste hydrolysate were analyzed for amino acid composition and molecular weight distribution. The samples of hydrolysate were used as core components for bacterial culture medium samples. The efficiency of the medium samples was tested for Escherichia coli growth rate; the most efficient sample had an efficiency of 95.3% of that of a commercially available medium based on fish meal. Substitution of medium components with those derived from industrial by-products is one of the ways to decrease a cost of a culture medium in biopharmaceutical drug production. |
format |
Text |
author |
Novikov, Vitaliy Yu. Shumskaya, Nadezhda V. Mukhin, Vyacheslav A. Zolotarev, Konstantin V. Mikhailov, Anton N. Nakhod, Valeriya I. Mikhailova, Marina V. |
author_facet |
Novikov, Vitaliy Yu. Shumskaya, Nadezhda V. Mukhin, Vyacheslav A. Zolotarev, Konstantin V. Mikhailov, Anton N. Nakhod, Valeriya I. Mikhailova, Marina V. |
author_sort |
Novikov, Vitaliy Yu. |
title |
Chemical Characterization of Atlantic Cod (Gadus morhua) Collagen Hydrolyzed Using Enzyme Preparation Derived from Red King Crab (Paralithodes camtschaticus) and Its Potential as a Core Component of Bacterial Culture Medium |
title_short |
Chemical Characterization of Atlantic Cod (Gadus morhua) Collagen Hydrolyzed Using Enzyme Preparation Derived from Red King Crab (Paralithodes camtschaticus) and Its Potential as a Core Component of Bacterial Culture Medium |
title_full |
Chemical Characterization of Atlantic Cod (Gadus morhua) Collagen Hydrolyzed Using Enzyme Preparation Derived from Red King Crab (Paralithodes camtschaticus) and Its Potential as a Core Component of Bacterial Culture Medium |
title_fullStr |
Chemical Characterization of Atlantic Cod (Gadus morhua) Collagen Hydrolyzed Using Enzyme Preparation Derived from Red King Crab (Paralithodes camtschaticus) and Its Potential as a Core Component of Bacterial Culture Medium |
title_full_unstemmed |
Chemical Characterization of Atlantic Cod (Gadus morhua) Collagen Hydrolyzed Using Enzyme Preparation Derived from Red King Crab (Paralithodes camtschaticus) and Its Potential as a Core Component of Bacterial Culture Medium |
title_sort |
chemical characterization of atlantic cod (gadus morhua) collagen hydrolyzed using enzyme preparation derived from red king crab (paralithodes camtschaticus) and its potential as a core component of bacterial culture medium |
publisher |
MDPI |
publishDate |
2021 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8399610/ http://www.ncbi.nlm.nih.gov/pubmed/34436311 https://doi.org/10.3390/md19080472 |
geographic |
Murmansk |
geographic_facet |
Murmansk |
genre |
atlantic cod Gadus morhua Paralithodes camtschaticus Red king crab |
genre_facet |
atlantic cod Gadus morhua Paralithodes camtschaticus Red king crab |
op_source |
Mar Drugs |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8399610/ http://www.ncbi.nlm.nih.gov/pubmed/34436311 http://dx.doi.org/10.3390/md19080472 |
op_rights |
© 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
op_doi |
https://doi.org/10.3390/md19080472 |
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Marine Drugs |
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19 |
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8 |
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472 |
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1766357421910917120 |