Candidatus Eremiobacterota, a metabolically and phylogenetically diverse terrestrial phylum with acid-tolerant adaptations
Candidatus phylum Eremiobacterota (formerly WPS-2) is an as-yet-uncultured bacterial clade that takes its name from Ca. Eremiobacter, an Antarctic soil aerobe proposed to be capable of a novel form of chemolithoautotrophy termed atmospheric chemosynthesis, that uses the energy derived from atmospher...
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ftpubmed:oai:pubmedcentral.nih.gov:8397712 2023-05-15T13:53:29+02:00 Candidatus Eremiobacterota, a metabolically and phylogenetically diverse terrestrial phylum with acid-tolerant adaptations Ji, Mukan Williams, Timothy J. Montgomery, Kate Wong, Hon Lun Zaugg, Julian Berengut, Jonathan F. Bissett, Andrew Chuvochina, Maria Hugenholtz, Philip Ferrari, Belinda C. 2021-03-22 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8397712/ http://www.ncbi.nlm.nih.gov/pubmed/33753881 https://doi.org/10.1038/s41396-021-00944-8 en eng Nature Publishing Group UK http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8397712/ http://www.ncbi.nlm.nih.gov/pubmed/33753881 http://dx.doi.org/10.1038/s41396-021-00944-8 © The Author(s), under exclusive licence to International Society for Microbial Ecology 2021 ISME J Article Text 2021 ftpubmed https://doi.org/10.1038/s41396-021-00944-8 2022-09-04T00:32:16Z Candidatus phylum Eremiobacterota (formerly WPS-2) is an as-yet-uncultured bacterial clade that takes its name from Ca. Eremiobacter, an Antarctic soil aerobe proposed to be capable of a novel form of chemolithoautotrophy termed atmospheric chemosynthesis, that uses the energy derived from atmospheric H(2)-oxidation to fix CO(2) through the Calvin-Benson-Bassham (CBB) cycle via type 1E RuBisCO. To elucidate the phylogenetic affiliation and metabolic capacities of Ca. Eremiobacterota, we analysed 63 public metagenome-assembled genomes (MAGs) and nine new MAGs generated from Antarctic soil metagenomes. These MAGs represent both recognized classes within Ca. Eremiobacterota, namely Ca. Eremiobacteria and UBP9. Ca. Eremiobacteria are inferred to be facultatively acidophilic with a preference for peptides and amino acids as nutrient sources. Epifluorescence microscopy revealed Ca. Eremiobacteria cells from Antarctica desert soil to be coccoid in shape. Two orders are recognized within class Ca. Eremiobacteria: Ca. Eremiobacterales and Ca. Baltobacterales. The latter are metabolically versatile, with individual members having genes required for trace gas driven autotrophy, anoxygenic photosynthesis, CO oxidation, and anaerobic respiration. UBP9, here renamed Ca. Xenobia class. nov., are inferred to be obligate heterotrophs with acidophilic adaptations, but individual members having highly divergent metabolic capacities compared to Ca. Eremiobacteria, especially with regard to respiration and central carbon metabolism. We conclude Ca. Eremiobacterota to be an ecologically versatile phylum with the potential to thrive under an array of “extreme” environmental conditions. Text Antarc* Antarctic Antarctica PubMed Central (PMC) Antarctic Calvin ENVELOPE(165.100,165.100,-71.283,-71.283) The ISME Journal 15 9 2692 2707 |
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Article Ji, Mukan Williams, Timothy J. Montgomery, Kate Wong, Hon Lun Zaugg, Julian Berengut, Jonathan F. Bissett, Andrew Chuvochina, Maria Hugenholtz, Philip Ferrari, Belinda C. Candidatus Eremiobacterota, a metabolically and phylogenetically diverse terrestrial phylum with acid-tolerant adaptations |
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Candidatus phylum Eremiobacterota (formerly WPS-2) is an as-yet-uncultured bacterial clade that takes its name from Ca. Eremiobacter, an Antarctic soil aerobe proposed to be capable of a novel form of chemolithoautotrophy termed atmospheric chemosynthesis, that uses the energy derived from atmospheric H(2)-oxidation to fix CO(2) through the Calvin-Benson-Bassham (CBB) cycle via type 1E RuBisCO. To elucidate the phylogenetic affiliation and metabolic capacities of Ca. Eremiobacterota, we analysed 63 public metagenome-assembled genomes (MAGs) and nine new MAGs generated from Antarctic soil metagenomes. These MAGs represent both recognized classes within Ca. Eremiobacterota, namely Ca. Eremiobacteria and UBP9. Ca. Eremiobacteria are inferred to be facultatively acidophilic with a preference for peptides and amino acids as nutrient sources. Epifluorescence microscopy revealed Ca. Eremiobacteria cells from Antarctica desert soil to be coccoid in shape. Two orders are recognized within class Ca. Eremiobacteria: Ca. Eremiobacterales and Ca. Baltobacterales. The latter are metabolically versatile, with individual members having genes required for trace gas driven autotrophy, anoxygenic photosynthesis, CO oxidation, and anaerobic respiration. UBP9, here renamed Ca. Xenobia class. nov., are inferred to be obligate heterotrophs with acidophilic adaptations, but individual members having highly divergent metabolic capacities compared to Ca. Eremiobacteria, especially with regard to respiration and central carbon metabolism. We conclude Ca. Eremiobacterota to be an ecologically versatile phylum with the potential to thrive under an array of “extreme” environmental conditions. |
format |
Text |
author |
Ji, Mukan Williams, Timothy J. Montgomery, Kate Wong, Hon Lun Zaugg, Julian Berengut, Jonathan F. Bissett, Andrew Chuvochina, Maria Hugenholtz, Philip Ferrari, Belinda C. |
author_facet |
Ji, Mukan Williams, Timothy J. Montgomery, Kate Wong, Hon Lun Zaugg, Julian Berengut, Jonathan F. Bissett, Andrew Chuvochina, Maria Hugenholtz, Philip Ferrari, Belinda C. |
author_sort |
Ji, Mukan |
title |
Candidatus Eremiobacterota, a metabolically and phylogenetically diverse terrestrial phylum with acid-tolerant adaptations |
title_short |
Candidatus Eremiobacterota, a metabolically and phylogenetically diverse terrestrial phylum with acid-tolerant adaptations |
title_full |
Candidatus Eremiobacterota, a metabolically and phylogenetically diverse terrestrial phylum with acid-tolerant adaptations |
title_fullStr |
Candidatus Eremiobacterota, a metabolically and phylogenetically diverse terrestrial phylum with acid-tolerant adaptations |
title_full_unstemmed |
Candidatus Eremiobacterota, a metabolically and phylogenetically diverse terrestrial phylum with acid-tolerant adaptations |
title_sort |
candidatus eremiobacterota, a metabolically and phylogenetically diverse terrestrial phylum with acid-tolerant adaptations |
publisher |
Nature Publishing Group UK |
publishDate |
2021 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8397712/ http://www.ncbi.nlm.nih.gov/pubmed/33753881 https://doi.org/10.1038/s41396-021-00944-8 |
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ENVELOPE(165.100,165.100,-71.283,-71.283) |
geographic |
Antarctic Calvin |
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Antarctic Calvin |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
ISME J |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8397712/ http://www.ncbi.nlm.nih.gov/pubmed/33753881 http://dx.doi.org/10.1038/s41396-021-00944-8 |
op_rights |
© The Author(s), under exclusive licence to International Society for Microbial Ecology 2021 |
op_doi |
https://doi.org/10.1038/s41396-021-00944-8 |
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The ISME Journal |
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15 |
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9 |
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2692 |
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2707 |
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