Quorum sensing in Aliivibrio wodanis 06/09/139 and its role in controlling various phenotypic traits
BACKGROUND: Quorum Sensing (QS) is a cell-to-cell communication system that bacteria utilize to adapt to the external environment by synthesizing and responding to signalling molecules called autoinducers. The psychrotrophic bacterium Aliivibrio wodanis 06/09/139, originally isolated from a winter u...
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ftpubmed:oai:pubmedcentral.nih.gov:8395575 2023-05-15T15:32:49+02:00 Quorum sensing in Aliivibrio wodanis 06/09/139 and its role in controlling various phenotypic traits Maharajan, Amudha Deepalakshmi Hansen, Hilde Khider, Miriam Willassen, Nils Peder 2021-08-24 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8395575/ https://doi.org/10.7717/peerj.11980 en eng PeerJ Inc. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8395575/ http://dx.doi.org/10.7717/peerj.11980 ©2021 Maharajan et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. CC-BY PeerJ Aquaculture Fisheries and Fish Science Text 2021 ftpubmed https://doi.org/10.7717/peerj.11980 2021-09-12T00:25:09Z BACKGROUND: Quorum Sensing (QS) is a cell-to-cell communication system that bacteria utilize to adapt to the external environment by synthesizing and responding to signalling molecules called autoinducers. The psychrotrophic bacterium Aliivibrio wodanis 06/09/139, originally isolated from a winter ulcer of a reared Atlantic salmon, produces the autoinducer N-3-hydroxy-decanoyl-homoserine-lactone (3OHC10-HSL) and encodes the QS systems AinS/R and LuxS/PQ, and the master regulator LitR. However, the role of QS in this bacterium has not been investigated yet. RESULTS: In the present work we show that 3OHC10-HSL production is cell density and temperature-dependent in A. wodanis 06/09/139 with the highest production occurring at a low temperature (6 °C). Gene inactivation demonstrates that AinS is responsible for 3OHC10-HSL production and positively regulated by LitR. Inactivation of ainS and litR further show that QS is involved in the regulation of growth, motility, hemolysis, protease activity and siderophore production. Of these QS regulated activities, only the protease activity was found to be independent of LitR. Lastly, supernatants harvested from the wild type and the ΔainS and ΔlitR mutants at high cell densities show that inactivation of QS leads to a decreased cytopathogenic effect (CPE) in a cell culture assay, and strongest attenuation of the CPE was observed with supernatants harvested from the ΔlitR mutant. CONCLUSION: A. wodanis 06/09/139 use QS to regulate a number of activities that may prove important for host colonization or interactions. The temperature of 6 °C that is in the temperature range at which winter ulcer occurs, plays a role in AHL production and development of CPE on a Chinook Salmon Embryo (CHSE) cell line. Text Atlantic salmon PubMed Central (PMC) PeerJ 9 e11980 |
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English |
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Aquaculture Fisheries and Fish Science |
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Aquaculture Fisheries and Fish Science Maharajan, Amudha Deepalakshmi Hansen, Hilde Khider, Miriam Willassen, Nils Peder Quorum sensing in Aliivibrio wodanis 06/09/139 and its role in controlling various phenotypic traits |
topic_facet |
Aquaculture Fisheries and Fish Science |
description |
BACKGROUND: Quorum Sensing (QS) is a cell-to-cell communication system that bacteria utilize to adapt to the external environment by synthesizing and responding to signalling molecules called autoinducers. The psychrotrophic bacterium Aliivibrio wodanis 06/09/139, originally isolated from a winter ulcer of a reared Atlantic salmon, produces the autoinducer N-3-hydroxy-decanoyl-homoserine-lactone (3OHC10-HSL) and encodes the QS systems AinS/R and LuxS/PQ, and the master regulator LitR. However, the role of QS in this bacterium has not been investigated yet. RESULTS: In the present work we show that 3OHC10-HSL production is cell density and temperature-dependent in A. wodanis 06/09/139 with the highest production occurring at a low temperature (6 °C). Gene inactivation demonstrates that AinS is responsible for 3OHC10-HSL production and positively regulated by LitR. Inactivation of ainS and litR further show that QS is involved in the regulation of growth, motility, hemolysis, protease activity and siderophore production. Of these QS regulated activities, only the protease activity was found to be independent of LitR. Lastly, supernatants harvested from the wild type and the ΔainS and ΔlitR mutants at high cell densities show that inactivation of QS leads to a decreased cytopathogenic effect (CPE) in a cell culture assay, and strongest attenuation of the CPE was observed with supernatants harvested from the ΔlitR mutant. CONCLUSION: A. wodanis 06/09/139 use QS to regulate a number of activities that may prove important for host colonization or interactions. The temperature of 6 °C that is in the temperature range at which winter ulcer occurs, plays a role in AHL production and development of CPE on a Chinook Salmon Embryo (CHSE) cell line. |
format |
Text |
author |
Maharajan, Amudha Deepalakshmi Hansen, Hilde Khider, Miriam Willassen, Nils Peder |
author_facet |
Maharajan, Amudha Deepalakshmi Hansen, Hilde Khider, Miriam Willassen, Nils Peder |
author_sort |
Maharajan, Amudha Deepalakshmi |
title |
Quorum sensing in Aliivibrio wodanis 06/09/139 and its role in controlling various phenotypic traits |
title_short |
Quorum sensing in Aliivibrio wodanis 06/09/139 and its role in controlling various phenotypic traits |
title_full |
Quorum sensing in Aliivibrio wodanis 06/09/139 and its role in controlling various phenotypic traits |
title_fullStr |
Quorum sensing in Aliivibrio wodanis 06/09/139 and its role in controlling various phenotypic traits |
title_full_unstemmed |
Quorum sensing in Aliivibrio wodanis 06/09/139 and its role in controlling various phenotypic traits |
title_sort |
quorum sensing in aliivibrio wodanis 06/09/139 and its role in controlling various phenotypic traits |
publisher |
PeerJ Inc. |
publishDate |
2021 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8395575/ https://doi.org/10.7717/peerj.11980 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
PeerJ |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8395575/ http://dx.doi.org/10.7717/peerj.11980 |
op_rights |
©2021 Maharajan et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
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CC-BY |
op_doi |
https://doi.org/10.7717/peerj.11980 |
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PeerJ |
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9 |
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e11980 |
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