Anti-Virulence Activity of the Cell-Free Supernatant of the Antarctic Bacterium Psychrobacter sp. TAE2020 against Pseudomonas aeruginosa Clinical Isolates from Cystic Fibrosis Patients
Pseudomonas aeruginosa is an opportunistic pathogen often involved in airway infections of cystic fibrosis (CF) patients. Its pathogenicity is related to several virulence factors, such as biofilm formation, motility and production of toxins and proteases. The expression of these virulence factors i...
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ftpubmed:oai:pubmedcentral.nih.gov:8388993 2023-05-15T14:01:45+02:00 Anti-Virulence Activity of the Cell-Free Supernatant of the Antarctic Bacterium Psychrobacter sp. TAE2020 against Pseudomonas aeruginosa Clinical Isolates from Cystic Fibrosis Patients Papa, Rosanna Vrenna, Gianluca D’Angelo, Caterina Casillo, Angela Relucenti, Michela Donfrancesco, Orlando Corsaro, Maria Michela Fiscarelli, Ersilia Vita Tuccio Guarna Assanti, Vanessa Tutino, Maria Luisa Parrilli, Ermenegilda Artini, Marco Selan, Laura 2021-08-04 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8388993/ https://doi.org/10.3390/antibiotics10080944 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8388993/ http://dx.doi.org/10.3390/antibiotics10080944 © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). CC-BY Antibiotics (Basel) Article Text 2021 ftpubmed https://doi.org/10.3390/antibiotics10080944 2021-08-29T00:49:44Z Pseudomonas aeruginosa is an opportunistic pathogen often involved in airway infections of cystic fibrosis (CF) patients. Its pathogenicity is related to several virulence factors, such as biofilm formation, motility and production of toxins and proteases. The expression of these virulence factors is controlled by quorum sensing (QS). Thus, QS inhibition is considered a novel strategy for the development of antipathogenic compounds acting on specific bacterial virulence programs without affecting bacterial vitality. In this context, cold-adapted marine bacteria living in polar regions represent an untapped reservoir of biodiversity endowed with an interesting chemical repertoire. In this paper, we investigated the biological activity of a supernatant derived from a novel Antarctic bacterium (SN_TAE2020) against specific virulence factors produced by P. aeruginosa strains isolated from FC patients. Our results clearly show a reduction in pyocyanin and protease production in the presence of SN_TAE2020. Finally, SN_TAE2020 was also able to strongly affect swarming and swimming motility for almost all tested strains. Furthermore, the effect of SN_TAE2020 was investigated on biofilm growth and texture, captured by SEM analysis. In consideration of the novel results obtained on clinical strains, polar bacteria might represent potential candidates for the discovery of new compounds limiting P. aeruginosa virulence in CF patients. Text Antarc* Antarctic PubMed Central (PMC) Antarctic The Antarctic Antibiotics 10 8 944 |
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Article Papa, Rosanna Vrenna, Gianluca D’Angelo, Caterina Casillo, Angela Relucenti, Michela Donfrancesco, Orlando Corsaro, Maria Michela Fiscarelli, Ersilia Vita Tuccio Guarna Assanti, Vanessa Tutino, Maria Luisa Parrilli, Ermenegilda Artini, Marco Selan, Laura Anti-Virulence Activity of the Cell-Free Supernatant of the Antarctic Bacterium Psychrobacter sp. TAE2020 against Pseudomonas aeruginosa Clinical Isolates from Cystic Fibrosis Patients |
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Article |
description |
Pseudomonas aeruginosa is an opportunistic pathogen often involved in airway infections of cystic fibrosis (CF) patients. Its pathogenicity is related to several virulence factors, such as biofilm formation, motility and production of toxins and proteases. The expression of these virulence factors is controlled by quorum sensing (QS). Thus, QS inhibition is considered a novel strategy for the development of antipathogenic compounds acting on specific bacterial virulence programs without affecting bacterial vitality. In this context, cold-adapted marine bacteria living in polar regions represent an untapped reservoir of biodiversity endowed with an interesting chemical repertoire. In this paper, we investigated the biological activity of a supernatant derived from a novel Antarctic bacterium (SN_TAE2020) against specific virulence factors produced by P. aeruginosa strains isolated from FC patients. Our results clearly show a reduction in pyocyanin and protease production in the presence of SN_TAE2020. Finally, SN_TAE2020 was also able to strongly affect swarming and swimming motility for almost all tested strains. Furthermore, the effect of SN_TAE2020 was investigated on biofilm growth and texture, captured by SEM analysis. In consideration of the novel results obtained on clinical strains, polar bacteria might represent potential candidates for the discovery of new compounds limiting P. aeruginosa virulence in CF patients. |
format |
Text |
author |
Papa, Rosanna Vrenna, Gianluca D’Angelo, Caterina Casillo, Angela Relucenti, Michela Donfrancesco, Orlando Corsaro, Maria Michela Fiscarelli, Ersilia Vita Tuccio Guarna Assanti, Vanessa Tutino, Maria Luisa Parrilli, Ermenegilda Artini, Marco Selan, Laura |
author_facet |
Papa, Rosanna Vrenna, Gianluca D’Angelo, Caterina Casillo, Angela Relucenti, Michela Donfrancesco, Orlando Corsaro, Maria Michela Fiscarelli, Ersilia Vita Tuccio Guarna Assanti, Vanessa Tutino, Maria Luisa Parrilli, Ermenegilda Artini, Marco Selan, Laura |
author_sort |
Papa, Rosanna |
title |
Anti-Virulence Activity of the Cell-Free Supernatant of the Antarctic Bacterium Psychrobacter sp. TAE2020 against Pseudomonas aeruginosa Clinical Isolates from Cystic Fibrosis Patients |
title_short |
Anti-Virulence Activity of the Cell-Free Supernatant of the Antarctic Bacterium Psychrobacter sp. TAE2020 against Pseudomonas aeruginosa Clinical Isolates from Cystic Fibrosis Patients |
title_full |
Anti-Virulence Activity of the Cell-Free Supernatant of the Antarctic Bacterium Psychrobacter sp. TAE2020 against Pseudomonas aeruginosa Clinical Isolates from Cystic Fibrosis Patients |
title_fullStr |
Anti-Virulence Activity of the Cell-Free Supernatant of the Antarctic Bacterium Psychrobacter sp. TAE2020 against Pseudomonas aeruginosa Clinical Isolates from Cystic Fibrosis Patients |
title_full_unstemmed |
Anti-Virulence Activity of the Cell-Free Supernatant of the Antarctic Bacterium Psychrobacter sp. TAE2020 against Pseudomonas aeruginosa Clinical Isolates from Cystic Fibrosis Patients |
title_sort |
anti-virulence activity of the cell-free supernatant of the antarctic bacterium psychrobacter sp. tae2020 against pseudomonas aeruginosa clinical isolates from cystic fibrosis patients |
publisher |
MDPI |
publishDate |
2021 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8388993/ https://doi.org/10.3390/antibiotics10080944 |
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Antarctic The Antarctic |
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Antarctic The Antarctic |
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Antarc* Antarctic |
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Antarc* Antarctic |
op_source |
Antibiotics (Basel) |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8388993/ http://dx.doi.org/10.3390/antibiotics10080944 |
op_rights |
© 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
op_doi |
https://doi.org/10.3390/antibiotics10080944 |
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Antibiotics |
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944 |
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