Sea-Ice Bacteria Halomonas sp. Strain 363 and Paracoccus sp. Strain 392 Produce Multiple Types of Poly-3-Hydroxyalkaonoic Acid (PHA) Storage Polymers at Low Temperature
Poly-3-hydroxyalkanoic acids (PHAs) are bacterial storage polymers commonly used in bioplastic production. Halophilic bacteria are industrially interesting organisms, as their salinity tolerance and psychrophilic nature lowers sterility requirements and subsequent production costs. We investigated P...
Published in: | Applied and Environmental Microbiology |
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2021
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8357295/ http://www.ncbi.nlm.nih.gov/pubmed/34160268 https://doi.org/10.1128/AEM.00929-21 |
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ftpubmed:oai:pubmedcentral.nih.gov:8357295 2023-05-15T18:17:31+02:00 Sea-Ice Bacteria Halomonas sp. Strain 363 and Paracoccus sp. Strain 392 Produce Multiple Types of Poly-3-Hydroxyalkaonoic Acid (PHA) Storage Polymers at Low Temperature Eronen-Rasimus, E. Hultman, J. Hai, T. Pessi, I. S. Collins, E. Wright, S. Laine, P. Viitamäki, S. Lyra, C. Thomas, D. N. Golyshin, P. N. Luhtanen, A.-M. Kuosa, H. Kaartokallio, H. 2021-08-11 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8357295/ http://www.ncbi.nlm.nih.gov/pubmed/34160268 https://doi.org/10.1128/AEM.00929-21 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8357295/ http://www.ncbi.nlm.nih.gov/pubmed/34160268 http://dx.doi.org/10.1128/AEM.00929-21 Copyright © 2021 Eronen-Rasimus et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . CC-BY Appl Environ Microbiol Microbial Ecology Text 2021 ftpubmed https://doi.org/10.1128/AEM.00929-21 2021-08-29T00:26:28Z Poly-3-hydroxyalkanoic acids (PHAs) are bacterial storage polymers commonly used in bioplastic production. Halophilic bacteria are industrially interesting organisms, as their salinity tolerance and psychrophilic nature lowers sterility requirements and subsequent production costs. We investigated PHA synthesis in two bacterial strains, Halomonas sp. 363 and Paracoccus sp. 392, isolated from Southern Ocean sea ice and elucidated the related PHA biopolymer accumulation and composition with various approaches, such as transcriptomics, microscopy, and chromatography. We show that both bacterial strains produce PHAs at 4°C when the availability of nitrogen and/or oxygen limited growth. The genome of Halomonas sp. 363 carries three phaC synthase genes and transcribes genes along three PHA pathways (I to III), whereas Paracoccus sp. 392 carries only one phaC gene and transcribes genes along one pathway (I). Thus, Halomonas sp. 363 has a versatile repertoire of phaC genes and pathways enabling production of both short- and medium-chain-length PHA products. IMPORTANCE Plastic pollution is one of the most topical threats to the health of the oceans and seas. One recognized way to alleviate the problem is to use degradable bioplastic materials in high-risk applications. PHA is a promising bioplastic material as it is nontoxic and fully produced and degraded by bacteria. Sea ice is an interesting environment for prospecting novel PHA-producing organisms, since traits advantageous to lower production costs, such as tolerance for high salinities and low temperatures, are common. We show that two sea-ice bacteria, Halomonas sp. 363 and Paracoccus sp. 392, are able to produce various types of PHA from inexpensive carbon sources. Halomonas sp. 363 is an especially interesting PHA-producing organism, since it has three different synthesis pathways to produce both short- and medium-chain-length PHAs. Text Sea ice Southern Ocean PubMed Central (PMC) Southern Ocean Applied and Environmental Microbiology 87 17 |
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PubMed Central (PMC) |
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language |
English |
topic |
Microbial Ecology |
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Microbial Ecology Eronen-Rasimus, E. Hultman, J. Hai, T. Pessi, I. S. Collins, E. Wright, S. Laine, P. Viitamäki, S. Lyra, C. Thomas, D. N. Golyshin, P. N. Luhtanen, A.-M. Kuosa, H. Kaartokallio, H. Sea-Ice Bacteria Halomonas sp. Strain 363 and Paracoccus sp. Strain 392 Produce Multiple Types of Poly-3-Hydroxyalkaonoic Acid (PHA) Storage Polymers at Low Temperature |
topic_facet |
Microbial Ecology |
description |
Poly-3-hydroxyalkanoic acids (PHAs) are bacterial storage polymers commonly used in bioplastic production. Halophilic bacteria are industrially interesting organisms, as their salinity tolerance and psychrophilic nature lowers sterility requirements and subsequent production costs. We investigated PHA synthesis in two bacterial strains, Halomonas sp. 363 and Paracoccus sp. 392, isolated from Southern Ocean sea ice and elucidated the related PHA biopolymer accumulation and composition with various approaches, such as transcriptomics, microscopy, and chromatography. We show that both bacterial strains produce PHAs at 4°C when the availability of nitrogen and/or oxygen limited growth. The genome of Halomonas sp. 363 carries three phaC synthase genes and transcribes genes along three PHA pathways (I to III), whereas Paracoccus sp. 392 carries only one phaC gene and transcribes genes along one pathway (I). Thus, Halomonas sp. 363 has a versatile repertoire of phaC genes and pathways enabling production of both short- and medium-chain-length PHA products. IMPORTANCE Plastic pollution is one of the most topical threats to the health of the oceans and seas. One recognized way to alleviate the problem is to use degradable bioplastic materials in high-risk applications. PHA is a promising bioplastic material as it is nontoxic and fully produced and degraded by bacteria. Sea ice is an interesting environment for prospecting novel PHA-producing organisms, since traits advantageous to lower production costs, such as tolerance for high salinities and low temperatures, are common. We show that two sea-ice bacteria, Halomonas sp. 363 and Paracoccus sp. 392, are able to produce various types of PHA from inexpensive carbon sources. Halomonas sp. 363 is an especially interesting PHA-producing organism, since it has three different synthesis pathways to produce both short- and medium-chain-length PHAs. |
format |
Text |
author |
Eronen-Rasimus, E. Hultman, J. Hai, T. Pessi, I. S. Collins, E. Wright, S. Laine, P. Viitamäki, S. Lyra, C. Thomas, D. N. Golyshin, P. N. Luhtanen, A.-M. Kuosa, H. Kaartokallio, H. |
author_facet |
Eronen-Rasimus, E. Hultman, J. Hai, T. Pessi, I. S. Collins, E. Wright, S. Laine, P. Viitamäki, S. Lyra, C. Thomas, D. N. Golyshin, P. N. Luhtanen, A.-M. Kuosa, H. Kaartokallio, H. |
author_sort |
Eronen-Rasimus, E. |
title |
Sea-Ice Bacteria Halomonas sp. Strain 363 and Paracoccus sp. Strain 392 Produce Multiple Types of Poly-3-Hydroxyalkaonoic Acid (PHA) Storage Polymers at Low Temperature |
title_short |
Sea-Ice Bacteria Halomonas sp. Strain 363 and Paracoccus sp. Strain 392 Produce Multiple Types of Poly-3-Hydroxyalkaonoic Acid (PHA) Storage Polymers at Low Temperature |
title_full |
Sea-Ice Bacteria Halomonas sp. Strain 363 and Paracoccus sp. Strain 392 Produce Multiple Types of Poly-3-Hydroxyalkaonoic Acid (PHA) Storage Polymers at Low Temperature |
title_fullStr |
Sea-Ice Bacteria Halomonas sp. Strain 363 and Paracoccus sp. Strain 392 Produce Multiple Types of Poly-3-Hydroxyalkaonoic Acid (PHA) Storage Polymers at Low Temperature |
title_full_unstemmed |
Sea-Ice Bacteria Halomonas sp. Strain 363 and Paracoccus sp. Strain 392 Produce Multiple Types of Poly-3-Hydroxyalkaonoic Acid (PHA) Storage Polymers at Low Temperature |
title_sort |
sea-ice bacteria halomonas sp. strain 363 and paracoccus sp. strain 392 produce multiple types of poly-3-hydroxyalkaonoic acid (pha) storage polymers at low temperature |
publisher |
American Society for Microbiology |
publishDate |
2021 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8357295/ http://www.ncbi.nlm.nih.gov/pubmed/34160268 https://doi.org/10.1128/AEM.00929-21 |
geographic |
Southern Ocean |
geographic_facet |
Southern Ocean |
genre |
Sea ice Southern Ocean |
genre_facet |
Sea ice Southern Ocean |
op_source |
Appl Environ Microbiol |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8357295/ http://www.ncbi.nlm.nih.gov/pubmed/34160268 http://dx.doi.org/10.1128/AEM.00929-21 |
op_rights |
Copyright © 2021 Eronen-Rasimus et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1128/AEM.00929-21 |
container_title |
Applied and Environmental Microbiology |
container_volume |
87 |
container_issue |
17 |
_version_ |
1766191775711494144 |