Vibrio aestuarianus subsp. cardii subsp. nov., pathogenic to the edible cockles Cerastoderma edule in France, and establishment of Vibrio aestuarianus subsp. aestuarianus subsp. nov. and Vibrio aestuarianus subsp. francensis subsp. nov.
Cockle mortality events have been reported in northern France since 2012. In the present study, we describe and investigate the implication of a potential bacterial causative agent in cockle mortality. Bacteria isolated from five different cockle mortality events were characterized and studied. Usin...
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ftpubmed:oai:pubmedcentral.nih.gov:8346768 2023-05-15T17:54:20+02:00 Vibrio aestuarianus subsp. cardii subsp. nov., pathogenic to the edible cockles Cerastoderma edule in France, and establishment of Vibrio aestuarianus subsp. aestuarianus subsp. nov. and Vibrio aestuarianus subsp. francensis subsp. nov. Garcia, Céline Mesnil, Aurélie Tourbiez, Delphine Moussa, Mirna Dubreuil, Christine Gonçalves de Sa, Amélie Chollet, Bruno Godfrin, Yoann Dégremont, Lionel Serpin, Delphine Travers, Marie-Agnès 2021-01-28 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8346768/ http://www.ncbi.nlm.nih.gov/pubmed/33507857 https://doi.org/10.1099/ijsem.0.004654 en eng Microbiology Society http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8346768/ http://www.ncbi.nlm.nih.gov/pubmed/33507857 http://dx.doi.org/10.1099/ijsem.0.004654 © 2021 The Authors https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License. CC-BY-NC Int J Syst Evol Microbiol Taxonomic Description Text 2021 ftpubmed https://doi.org/10.1099/ijsem.0.004654 2021-08-15T00:36:37Z Cockle mortality events have been reported in northern France since 2012. In the present study, we describe and investigate the implication of a potential bacterial causative agent in cockle mortality. Bacteria isolated from five different cockle mortality events were characterized and studied. Using phenotypic analysis combined with DNA–DNA hybridization (DDH) and whole genome sequencing, the isolates were shown to belong to Vibrio aestuarianus , a species regularly detected in France during oyster mortality events. Comparison of the strains from cockles with strains from French oysters and the type strain showed that the strains from cockles were genetically different to those from oysters and also different to the V. aestuarianus type strain. Moreover, the cockle and oyster strains were classified into two different, but close, groups both separated from the type strain by: (1) analyses of the ldh gene sequences; (2) DDH assays between 12/122 3T3(T) (LMG 31436(T)=DSM 109723(T)), a representative cockle strain, 02/041(T) (CIP 109791(T)=LMG 24517(T)) representative oyster strain and V. aestuarianus type strain LMG 7909(T); (3) average nucleotide identity values calculated on the genomes; and (4) phenotypic traits. Finally, results of MALDI-TOF analyses also revealed specific peaks discriminating the three representative strains. The toxicity of representative strains of these cockle isolates was demonstrated by experimental infection of hatchery-produced cockles. The data therefore allow us to propose two novel subspecies of Vibrio aestuarianus : Vibrio aestuarianus subsp. cardii subsp. nov. for the cockle strains and Vibrio aestuarianus subsp. francensis subsp. nov. for the Pacific oyster strains, in addition to an emended description of the species Vibrio aestuarianus . Text Pacific oyster PubMed Central (PMC) Pacific International Journal of Systematic and Evolutionary Microbiology 71 2 |
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Taxonomic Description |
spellingShingle |
Taxonomic Description Garcia, Céline Mesnil, Aurélie Tourbiez, Delphine Moussa, Mirna Dubreuil, Christine Gonçalves de Sa, Amélie Chollet, Bruno Godfrin, Yoann Dégremont, Lionel Serpin, Delphine Travers, Marie-Agnès Vibrio aestuarianus subsp. cardii subsp. nov., pathogenic to the edible cockles Cerastoderma edule in France, and establishment of Vibrio aestuarianus subsp. aestuarianus subsp. nov. and Vibrio aestuarianus subsp. francensis subsp. nov. |
topic_facet |
Taxonomic Description |
description |
Cockle mortality events have been reported in northern France since 2012. In the present study, we describe and investigate the implication of a potential bacterial causative agent in cockle mortality. Bacteria isolated from five different cockle mortality events were characterized and studied. Using phenotypic analysis combined with DNA–DNA hybridization (DDH) and whole genome sequencing, the isolates were shown to belong to Vibrio aestuarianus , a species regularly detected in France during oyster mortality events. Comparison of the strains from cockles with strains from French oysters and the type strain showed that the strains from cockles were genetically different to those from oysters and also different to the V. aestuarianus type strain. Moreover, the cockle and oyster strains were classified into two different, but close, groups both separated from the type strain by: (1) analyses of the ldh gene sequences; (2) DDH assays between 12/122 3T3(T) (LMG 31436(T)=DSM 109723(T)), a representative cockle strain, 02/041(T) (CIP 109791(T)=LMG 24517(T)) representative oyster strain and V. aestuarianus type strain LMG 7909(T); (3) average nucleotide identity values calculated on the genomes; and (4) phenotypic traits. Finally, results of MALDI-TOF analyses also revealed specific peaks discriminating the three representative strains. The toxicity of representative strains of these cockle isolates was demonstrated by experimental infection of hatchery-produced cockles. The data therefore allow us to propose two novel subspecies of Vibrio aestuarianus : Vibrio aestuarianus subsp. cardii subsp. nov. for the cockle strains and Vibrio aestuarianus subsp. francensis subsp. nov. for the Pacific oyster strains, in addition to an emended description of the species Vibrio aestuarianus . |
format |
Text |
author |
Garcia, Céline Mesnil, Aurélie Tourbiez, Delphine Moussa, Mirna Dubreuil, Christine Gonçalves de Sa, Amélie Chollet, Bruno Godfrin, Yoann Dégremont, Lionel Serpin, Delphine Travers, Marie-Agnès |
author_facet |
Garcia, Céline Mesnil, Aurélie Tourbiez, Delphine Moussa, Mirna Dubreuil, Christine Gonçalves de Sa, Amélie Chollet, Bruno Godfrin, Yoann Dégremont, Lionel Serpin, Delphine Travers, Marie-Agnès |
author_sort |
Garcia, Céline |
title |
Vibrio aestuarianus subsp. cardii subsp. nov., pathogenic to the edible cockles Cerastoderma edule in France, and establishment of Vibrio aestuarianus subsp. aestuarianus subsp. nov. and Vibrio aestuarianus subsp. francensis subsp. nov. |
title_short |
Vibrio aestuarianus subsp. cardii subsp. nov., pathogenic to the edible cockles Cerastoderma edule in France, and establishment of Vibrio aestuarianus subsp. aestuarianus subsp. nov. and Vibrio aestuarianus subsp. francensis subsp. nov. |
title_full |
Vibrio aestuarianus subsp. cardii subsp. nov., pathogenic to the edible cockles Cerastoderma edule in France, and establishment of Vibrio aestuarianus subsp. aestuarianus subsp. nov. and Vibrio aestuarianus subsp. francensis subsp. nov. |
title_fullStr |
Vibrio aestuarianus subsp. cardii subsp. nov., pathogenic to the edible cockles Cerastoderma edule in France, and establishment of Vibrio aestuarianus subsp. aestuarianus subsp. nov. and Vibrio aestuarianus subsp. francensis subsp. nov. |
title_full_unstemmed |
Vibrio aestuarianus subsp. cardii subsp. nov., pathogenic to the edible cockles Cerastoderma edule in France, and establishment of Vibrio aestuarianus subsp. aestuarianus subsp. nov. and Vibrio aestuarianus subsp. francensis subsp. nov. |
title_sort |
vibrio aestuarianus subsp. cardii subsp. nov., pathogenic to the edible cockles cerastoderma edule in france, and establishment of vibrio aestuarianus subsp. aestuarianus subsp. nov. and vibrio aestuarianus subsp. francensis subsp. nov. |
publisher |
Microbiology Society |
publishDate |
2021 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8346768/ http://www.ncbi.nlm.nih.gov/pubmed/33507857 https://doi.org/10.1099/ijsem.0.004654 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Pacific oyster |
genre_facet |
Pacific oyster |
op_source |
Int J Syst Evol Microbiol |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8346768/ http://www.ncbi.nlm.nih.gov/pubmed/33507857 http://dx.doi.org/10.1099/ijsem.0.004654 |
op_rights |
© 2021 The Authors https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License. |
op_rightsnorm |
CC-BY-NC |
op_doi |
https://doi.org/10.1099/ijsem.0.004654 |
container_title |
International Journal of Systematic and Evolutionary Microbiology |
container_volume |
71 |
container_issue |
2 |
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1766162091609161728 |