Novel reassortant 2.3.4.4B H5N6 highly pathogenic avian influenza viruses circulating among wild, domestic birds in Xinjiang, Northwest China
BACKGROUND: The H5 avian influenza viruses (AIVs) of clade 2.3.4.4 circulate in wild and domestic birds worldwide. In 2017, nine strains of H5N6 AIVs were isolated from aquatic poultry in Xinjiang, Northwest China. OBJECTIVES: This study aimed to analyze the origin, reassortment, and mutations of th...
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ftpubmed:oai:pubmedcentral.nih.gov:8318794 2023-05-15T18:19:24+02:00 Novel reassortant 2.3.4.4B H5N6 highly pathogenic avian influenza viruses circulating among wild, domestic birds in Xinjiang, Northwest China Zhang, Qian Mei, Xindi Zhang, Cheng Li, Juan Chang, Nana Aji, Dilihuma Shi, Weifeng Bi, Yuhai Ma, Zhenghai 2021-07 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8318794/ http://www.ncbi.nlm.nih.gov/pubmed/34170087 https://doi.org/10.4142/jvs.2021.22.e43 en eng The Korean Society of Veterinary Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8318794/ http://www.ncbi.nlm.nih.gov/pubmed/34170087 http://dx.doi.org/10.4142/jvs.2021.22.e43 © 2021 The Korean Society of Veterinary Science https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. CC-BY-NC J Vet Sci Original Article Text 2021 ftpubmed https://doi.org/10.4142/jvs.2021.22.e43 2021-08-08T00:42:33Z BACKGROUND: The H5 avian influenza viruses (AIVs) of clade 2.3.4.4 circulate in wild and domestic birds worldwide. In 2017, nine strains of H5N6 AIVs were isolated from aquatic poultry in Xinjiang, Northwest China. OBJECTIVES: This study aimed to analyze the origin, reassortment, and mutations of the AIV isolates. METHODS: AIVs were isolated from oropharyngeal and cloacal swabs of poultry. Identification was accomplished by inoculating isolates into embryonated chicken eggs and performing hemagglutination tests and reverse transcription polymerase chain reaction (RT-PCR). The viral genomes were amplified with RT-PCR and then sequenced. The sequence alignment, phylogenetic, and molecular characteristic analyses were performed by using bioinformatic software. RESULTS: Nine isolates originated from the same ancestor. The viral HA gene belonged to clade 2.3.4.4B, while the NA gene had a close phylogenetic relationship with the 2.3.4.4C H5N6 highly pathogenic avian influenza viruses (HPAIVs) isolated from shoveler ducks in Ningxia in 2015. The NP gene was grouped into an independent subcluster within the 2.3.4.4B H5N8 AIVs, and the remaining six genes all had close phylogenetic relationships with the 2.3.4.4B H5N8 HPAIVs isolated from the wild birds in China, Egypt, Uganda, Cameroon, and India in 2016–2017, Multiple basic amino acid residues associated with HPAIVs were located adjacent to the cleavage site of the HA protein. The nine isolates comprised reassortant 2.3.4.4B HPAIVs originating from 2.3.4.4B H5N8 and 2.3.4.4C H5N6 viruses in wild birds. CONCLUSIONS: These results suggest that the Northern Tianshan Mountain wetlands in Xinjiang may have a key role in AIVs disseminating from Central China to the Eurasian continent and East African. Text Shoveler PubMed Central (PMC) Journal of Veterinary Science 22 4 |
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Original Article Zhang, Qian Mei, Xindi Zhang, Cheng Li, Juan Chang, Nana Aji, Dilihuma Shi, Weifeng Bi, Yuhai Ma, Zhenghai Novel reassortant 2.3.4.4B H5N6 highly pathogenic avian influenza viruses circulating among wild, domestic birds in Xinjiang, Northwest China |
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Original Article |
description |
BACKGROUND: The H5 avian influenza viruses (AIVs) of clade 2.3.4.4 circulate in wild and domestic birds worldwide. In 2017, nine strains of H5N6 AIVs were isolated from aquatic poultry in Xinjiang, Northwest China. OBJECTIVES: This study aimed to analyze the origin, reassortment, and mutations of the AIV isolates. METHODS: AIVs were isolated from oropharyngeal and cloacal swabs of poultry. Identification was accomplished by inoculating isolates into embryonated chicken eggs and performing hemagglutination tests and reverse transcription polymerase chain reaction (RT-PCR). The viral genomes were amplified with RT-PCR and then sequenced. The sequence alignment, phylogenetic, and molecular characteristic analyses were performed by using bioinformatic software. RESULTS: Nine isolates originated from the same ancestor. The viral HA gene belonged to clade 2.3.4.4B, while the NA gene had a close phylogenetic relationship with the 2.3.4.4C H5N6 highly pathogenic avian influenza viruses (HPAIVs) isolated from shoveler ducks in Ningxia in 2015. The NP gene was grouped into an independent subcluster within the 2.3.4.4B H5N8 AIVs, and the remaining six genes all had close phylogenetic relationships with the 2.3.4.4B H5N8 HPAIVs isolated from the wild birds in China, Egypt, Uganda, Cameroon, and India in 2016–2017, Multiple basic amino acid residues associated with HPAIVs were located adjacent to the cleavage site of the HA protein. The nine isolates comprised reassortant 2.3.4.4B HPAIVs originating from 2.3.4.4B H5N8 and 2.3.4.4C H5N6 viruses in wild birds. CONCLUSIONS: These results suggest that the Northern Tianshan Mountain wetlands in Xinjiang may have a key role in AIVs disseminating from Central China to the Eurasian continent and East African. |
format |
Text |
author |
Zhang, Qian Mei, Xindi Zhang, Cheng Li, Juan Chang, Nana Aji, Dilihuma Shi, Weifeng Bi, Yuhai Ma, Zhenghai |
author_facet |
Zhang, Qian Mei, Xindi Zhang, Cheng Li, Juan Chang, Nana Aji, Dilihuma Shi, Weifeng Bi, Yuhai Ma, Zhenghai |
author_sort |
Zhang, Qian |
title |
Novel reassortant 2.3.4.4B H5N6 highly pathogenic avian influenza viruses circulating among wild, domestic birds in Xinjiang, Northwest China |
title_short |
Novel reassortant 2.3.4.4B H5N6 highly pathogenic avian influenza viruses circulating among wild, domestic birds in Xinjiang, Northwest China |
title_full |
Novel reassortant 2.3.4.4B H5N6 highly pathogenic avian influenza viruses circulating among wild, domestic birds in Xinjiang, Northwest China |
title_fullStr |
Novel reassortant 2.3.4.4B H5N6 highly pathogenic avian influenza viruses circulating among wild, domestic birds in Xinjiang, Northwest China |
title_full_unstemmed |
Novel reassortant 2.3.4.4B H5N6 highly pathogenic avian influenza viruses circulating among wild, domestic birds in Xinjiang, Northwest China |
title_sort |
novel reassortant 2.3.4.4b h5n6 highly pathogenic avian influenza viruses circulating among wild, domestic birds in xinjiang, northwest china |
publisher |
The Korean Society of Veterinary Science |
publishDate |
2021 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8318794/ http://www.ncbi.nlm.nih.gov/pubmed/34170087 https://doi.org/10.4142/jvs.2021.22.e43 |
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Shoveler |
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Shoveler |
op_source |
J Vet Sci |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8318794/ http://www.ncbi.nlm.nih.gov/pubmed/34170087 http://dx.doi.org/10.4142/jvs.2021.22.e43 |
op_rights |
© 2021 The Korean Society of Veterinary Science https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
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CC-BY-NC |
op_doi |
https://doi.org/10.4142/jvs.2021.22.e43 |
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Journal of Veterinary Science |
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22 |
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4 |
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1766196468749697024 |