Investigation of the occurrence of Angiostrongylus vasorum in coyotes in southern Ontario, Canada
In North America, the only endemic focus for Angiostrongylus vasorum (French heartworm) was historically thought to occur in the southeastern part of the island of Newfoundland. However, reports of A. vasorum infection in wild canids in West Virginia, USA, and Nova Scotia, Canada, suggest the introd...
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ftpubmed:oai:pubmedcentral.nih.gov:8225693 2023-05-15T17:22:41+02:00 Investigation of the occurrence of Angiostrongylus vasorum in coyotes in southern Ontario, Canada Kotwa, Jonathon D. Schnyder, Manuela Jardine, Claire M. Deplazes, Peter Pearl, David L. Berke, Olaf Mercer, Nicola Peregrine, Andrew S. 2021-04-23 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8225693/ http://www.ncbi.nlm.nih.gov/pubmed/33890522 https://doi.org/10.1177/10406387211011990 en eng SAGE Publications http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8225693/ http://www.ncbi.nlm.nih.gov/pubmed/33890522 http://dx.doi.org/10.1177/10406387211011990 © 2021 The Author(s) https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage). CC-BY-NC J Vet Diagn Invest Full Scientific Reports Text 2021 ftpubmed https://doi.org/10.1177/10406387211011990 2021-07-04T00:46:52Z In North America, the only endemic focus for Angiostrongylus vasorum (French heartworm) was historically thought to occur in the southeastern part of the island of Newfoundland. However, reports of A. vasorum infection in wild canids in West Virginia, USA, and Nova Scotia, Canada, suggest the introduction of the parasite to mainland North America. We screened for A. vasorum in coyotes from across southern Ontario. Additionally, we evaluated the performance of ELISAs for detection of circulating A. vasorum antigen (Ag-ELISA) and antibodies against A. vasorum (Ab-ELISA) designed for use in sera or blood of foxes for use with coyotes in this region. Autopsies were performed on 397 coyotes, and lung tissue extract prepared from each carcass was tested via both ELISAs. The sensitivity and specificity for both tests were estimated in the absence of a gold standard using a 2-test single population Bayesian model; sensitivity and specificity priors were based on the performance of the assays in foxes in Switzerland. Eight coyotes tested positive for A. vasorum antigen; no animal was antibody positive. The estimated sensitivity and specificity of the Ag-ELISA were 90.8% (95% credible interval [CrI]: 83.8–95.6%) and 95.5% (95% CrI: 93.4–97.2%), respectively. For the Ab-ELISA, the estimated sensitivity and specificity were 41.9% (95% CrI: 32.1–51.9%) and 98.0% (95% CrI: 96.3–99.0%), respectively. Based on these findings and negative postmortem data for the same animals, there is insufficient evidence to suggest the presence of A. vasorum in southern Ontario coyotes. Text Newfoundland PubMed Central (PMC) Canada Journal of Veterinary Diagnostic Investigation 33 4 664 669 |
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Full Scientific Reports |
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Full Scientific Reports Kotwa, Jonathon D. Schnyder, Manuela Jardine, Claire M. Deplazes, Peter Pearl, David L. Berke, Olaf Mercer, Nicola Peregrine, Andrew S. Investigation of the occurrence of Angiostrongylus vasorum in coyotes in southern Ontario, Canada |
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Full Scientific Reports |
description |
In North America, the only endemic focus for Angiostrongylus vasorum (French heartworm) was historically thought to occur in the southeastern part of the island of Newfoundland. However, reports of A. vasorum infection in wild canids in West Virginia, USA, and Nova Scotia, Canada, suggest the introduction of the parasite to mainland North America. We screened for A. vasorum in coyotes from across southern Ontario. Additionally, we evaluated the performance of ELISAs for detection of circulating A. vasorum antigen (Ag-ELISA) and antibodies against A. vasorum (Ab-ELISA) designed for use in sera or blood of foxes for use with coyotes in this region. Autopsies were performed on 397 coyotes, and lung tissue extract prepared from each carcass was tested via both ELISAs. The sensitivity and specificity for both tests were estimated in the absence of a gold standard using a 2-test single population Bayesian model; sensitivity and specificity priors were based on the performance of the assays in foxes in Switzerland. Eight coyotes tested positive for A. vasorum antigen; no animal was antibody positive. The estimated sensitivity and specificity of the Ag-ELISA were 90.8% (95% credible interval [CrI]: 83.8–95.6%) and 95.5% (95% CrI: 93.4–97.2%), respectively. For the Ab-ELISA, the estimated sensitivity and specificity were 41.9% (95% CrI: 32.1–51.9%) and 98.0% (95% CrI: 96.3–99.0%), respectively. Based on these findings and negative postmortem data for the same animals, there is insufficient evidence to suggest the presence of A. vasorum in southern Ontario coyotes. |
format |
Text |
author |
Kotwa, Jonathon D. Schnyder, Manuela Jardine, Claire M. Deplazes, Peter Pearl, David L. Berke, Olaf Mercer, Nicola Peregrine, Andrew S. |
author_facet |
Kotwa, Jonathon D. Schnyder, Manuela Jardine, Claire M. Deplazes, Peter Pearl, David L. Berke, Olaf Mercer, Nicola Peregrine, Andrew S. |
author_sort |
Kotwa, Jonathon D. |
title |
Investigation of the occurrence of Angiostrongylus vasorum in coyotes in southern Ontario, Canada |
title_short |
Investigation of the occurrence of Angiostrongylus vasorum in coyotes in southern Ontario, Canada |
title_full |
Investigation of the occurrence of Angiostrongylus vasorum in coyotes in southern Ontario, Canada |
title_fullStr |
Investigation of the occurrence of Angiostrongylus vasorum in coyotes in southern Ontario, Canada |
title_full_unstemmed |
Investigation of the occurrence of Angiostrongylus vasorum in coyotes in southern Ontario, Canada |
title_sort |
investigation of the occurrence of angiostrongylus vasorum in coyotes in southern ontario, canada |
publisher |
SAGE Publications |
publishDate |
2021 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8225693/ http://www.ncbi.nlm.nih.gov/pubmed/33890522 https://doi.org/10.1177/10406387211011990 |
geographic |
Canada |
geographic_facet |
Canada |
genre |
Newfoundland |
genre_facet |
Newfoundland |
op_source |
J Vet Diagn Invest |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8225693/ http://www.ncbi.nlm.nih.gov/pubmed/33890522 http://dx.doi.org/10.1177/10406387211011990 |
op_rights |
© 2021 The Author(s) https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
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CC-BY-NC |
op_doi |
https://doi.org/10.1177/10406387211011990 |
container_title |
Journal of Veterinary Diagnostic Investigation |
container_volume |
33 |
container_issue |
4 |
container_start_page |
664 |
op_container_end_page |
669 |
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1766109503572410368 |