The cGAS/STING–TBK1–IRF Regulatory Axis Orchestrates a Primitive Interferon-Like Antiviral Mechanism in Oyster

Interferon (IFN) system is considered as the first defense line against viral infection, and it has been extensively studied in vertebrates from fish to mammals. In invertebrates, Vagos from arthropod and IFN-like protein (CgIFNLP) from Crassostrea gigas appeared to function as IFN-like antiviral cy...

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Published in:Frontiers in Immunology
Main Authors: Qiao, Xue, Zong, Yanan, Liu, Zhaoqun, Wu, Zhaojun, Li, Yuanmei, Wang, Lingling, Song, Linsheng
Format: Text
Language:English
Published: Frontiers Media S.A. 2021
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Online Access:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8218723/
https://doi.org/10.3389/fimmu.2021.689783
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spelling ftpubmed:oai:pubmedcentral.nih.gov:8218723 2023-05-15T15:58:47+02:00 The cGAS/STING–TBK1–IRF Regulatory Axis Orchestrates a Primitive Interferon-Like Antiviral Mechanism in Oyster Qiao, Xue Zong, Yanan Liu, Zhaoqun Wu, Zhaojun Li, Yuanmei Wang, Lingling Song, Linsheng 2021-06-08 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8218723/ https://doi.org/10.3389/fimmu.2021.689783 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8218723/ http://dx.doi.org/10.3389/fimmu.2021.689783 Copyright © 2021 Qiao, Zong, Liu, Wu, Li, Wang and Song https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. CC-BY Front Immunol Immunology Text 2021 ftpubmed https://doi.org/10.3389/fimmu.2021.689783 2021-06-27T00:39:56Z Interferon (IFN) system is considered as the first defense line against viral infection, and it has been extensively studied in vertebrates from fish to mammals. In invertebrates, Vagos from arthropod and IFN-like protein (CgIFNLP) from Crassostrea gigas appeared to function as IFN-like antiviral cytokines. In the present study, the CgIFNLP protein in hemocytes was observed to increase after Poly (I:C) stimulation. After CgIFNLP was knocked down by RNAi, the mRNA expression of IFN-stimulated genes (CgISGs) was significantly inhibited. Both cyclic GMP-AMP synthase (CgcGAS) and stimulator of interferon gene (CgSTING) identified from oyster were able to recognize the double-stranded nucleic acid [Poly (I:C) and dsDNA] and expressed at high level after Poly (I:C) stimulation. The expression of CgIFNLP and interferon regulatory factors (CgIRF1/8) and the nuclear translocation of CgIRF8 were all suppressed in CgcGAS-RNAi or CgSTING-RNAi oysters after Poly (I:C) stimulation. The expression level of CgSTING and TANK binding kinase1 (CgTBK1) did not decrease in CgcGAS-RNAi oysters. After CgSTING was knocked down, the high expression of CgTBK1 induced by Poly (I:C) was prevented significantly. These results indicated that there was a primitive IFN-like antiviral mechanism dependent on the cGAS/STING–TBK1–IRFs regulatory axis in mollusks, which was different from the classic cGAS–STING–TBK1 signal pathway in mammals. Text Crassostrea gigas PubMed Central (PMC) Frontiers in Immunology 12
institution Open Polar
collection PubMed Central (PMC)
op_collection_id ftpubmed
language English
topic Immunology
spellingShingle Immunology
Qiao, Xue
Zong, Yanan
Liu, Zhaoqun
Wu, Zhaojun
Li, Yuanmei
Wang, Lingling
Song, Linsheng
The cGAS/STING–TBK1–IRF Regulatory Axis Orchestrates a Primitive Interferon-Like Antiviral Mechanism in Oyster
topic_facet Immunology
description Interferon (IFN) system is considered as the first defense line against viral infection, and it has been extensively studied in vertebrates from fish to mammals. In invertebrates, Vagos from arthropod and IFN-like protein (CgIFNLP) from Crassostrea gigas appeared to function as IFN-like antiviral cytokines. In the present study, the CgIFNLP protein in hemocytes was observed to increase after Poly (I:C) stimulation. After CgIFNLP was knocked down by RNAi, the mRNA expression of IFN-stimulated genes (CgISGs) was significantly inhibited. Both cyclic GMP-AMP synthase (CgcGAS) and stimulator of interferon gene (CgSTING) identified from oyster were able to recognize the double-stranded nucleic acid [Poly (I:C) and dsDNA] and expressed at high level after Poly (I:C) stimulation. The expression of CgIFNLP and interferon regulatory factors (CgIRF1/8) and the nuclear translocation of CgIRF8 were all suppressed in CgcGAS-RNAi or CgSTING-RNAi oysters after Poly (I:C) stimulation. The expression level of CgSTING and TANK binding kinase1 (CgTBK1) did not decrease in CgcGAS-RNAi oysters. After CgSTING was knocked down, the high expression of CgTBK1 induced by Poly (I:C) was prevented significantly. These results indicated that there was a primitive IFN-like antiviral mechanism dependent on the cGAS/STING–TBK1–IRFs regulatory axis in mollusks, which was different from the classic cGAS–STING–TBK1 signal pathway in mammals.
format Text
author Qiao, Xue
Zong, Yanan
Liu, Zhaoqun
Wu, Zhaojun
Li, Yuanmei
Wang, Lingling
Song, Linsheng
author_facet Qiao, Xue
Zong, Yanan
Liu, Zhaoqun
Wu, Zhaojun
Li, Yuanmei
Wang, Lingling
Song, Linsheng
author_sort Qiao, Xue
title The cGAS/STING–TBK1–IRF Regulatory Axis Orchestrates a Primitive Interferon-Like Antiviral Mechanism in Oyster
title_short The cGAS/STING–TBK1–IRF Regulatory Axis Orchestrates a Primitive Interferon-Like Antiviral Mechanism in Oyster
title_full The cGAS/STING–TBK1–IRF Regulatory Axis Orchestrates a Primitive Interferon-Like Antiviral Mechanism in Oyster
title_fullStr The cGAS/STING–TBK1–IRF Regulatory Axis Orchestrates a Primitive Interferon-Like Antiviral Mechanism in Oyster
title_full_unstemmed The cGAS/STING–TBK1–IRF Regulatory Axis Orchestrates a Primitive Interferon-Like Antiviral Mechanism in Oyster
title_sort cgas/sting–tbk1–irf regulatory axis orchestrates a primitive interferon-like antiviral mechanism in oyster
publisher Frontiers Media S.A.
publishDate 2021
url http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8218723/
https://doi.org/10.3389/fimmu.2021.689783
genre Crassostrea gigas
genre_facet Crassostrea gigas
op_source Front Immunol
op_relation http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8218723/
http://dx.doi.org/10.3389/fimmu.2021.689783
op_rights Copyright © 2021 Qiao, Zong, Liu, Wu, Li, Wang and Song
https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
op_rightsnorm CC-BY
op_doi https://doi.org/10.3389/fimmu.2021.689783
container_title Frontiers in Immunology
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