Lactic acid production by Carnobacterium sp. isolated from a maritime Antarctic lake using eucalyptus enzymatic hydrolysate

• Published in: Biotechnology Reports
• Main Authors: Camesasca, Laura, de Mattos, Juan Andrés, Vila, Eugenia, Cebreiros, Florencia, Lareo, Claudia
• Format: Text
• Language: English
• Published: Elsevier 2021
• Subjects: Research Article; Antarctic; Antarc*
• Online Access: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209079/; https://doi.org/10.1016/j.btre.2021.e00643

Carnobacterium sp., a lactic acid bacterium isolated from a maritime Antarctic lake, was evaluated for lactic acid production from a lignocellulosic hydrolysate. Eucalyptus sawdust, a residue from pulp and paper industries, was subjected to alkaline pretreatment to enhance its enzymatic hydrolysis. Fermentations were performed without and with pH control using eucalyptus enzymatic hydrolysate containing a mixture of glucose and xylose sugars. The sugars were successfully converted into lactic acid in 24 h, resulting in 7.6 g/L of lactic acid and a product yield of 0.50 g/g for pH controlled at 6.5. Fed-batch fermentation performed at a controlled pH of 6.5 improved both the lactic acid production (30 g/L) and the biomass growth (4.2 g/L). l-lactic acid optical purity higher than 95 % was obtained. These results demonstrated the potential usage of Carnobacterium sp in l-lactic acid production from eucalyptus.