Metagenomic analysis of fecal and tissue samples from 18 endemic bat species in Switzerland revealed a diverse virus composition including potentially zoonotic viruses
Many recent disease outbreaks in humans had a zoonotic virus etiology. Bats in particular have been recognized as reservoirs to a large variety of viruses with the potential to cross-species transmission. In order to assess the risk of bats in Switzerland for such transmissions, we determined the vi...
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ftpubmed:oai:pubmedcentral.nih.gov:8208571 2023-05-15T18:42:44+02:00 Metagenomic analysis of fecal and tissue samples from 18 endemic bat species in Switzerland revealed a diverse virus composition including potentially zoonotic viruses Hardmeier, Isabelle Aeberhard, Nadja Qi, Weihong Schoenbaechler, Katja Kraettli, Hubert Hatt, Jean-Michel Fraefel, Cornel Kubacki, Jakub 2021-06-16 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8208571/ http://www.ncbi.nlm.nih.gov/pubmed/34133435 https://doi.org/10.1371/journal.pone.0252534 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8208571/ http://www.ncbi.nlm.nih.gov/pubmed/34133435 http://dx.doi.org/10.1371/journal.pone.0252534 © 2021 Hardmeier et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. CC-BY PLoS One Research Article Text 2021 ftpubmed https://doi.org/10.1371/journal.pone.0252534 2021-07-04T00:37:29Z Many recent disease outbreaks in humans had a zoonotic virus etiology. Bats in particular have been recognized as reservoirs to a large variety of viruses with the potential to cross-species transmission. In order to assess the risk of bats in Switzerland for such transmissions, we determined the virome of tissue and fecal samples of 14 native and 4 migrating bat species. In total, sequences belonging to 39 different virus families, 16 of which are known to infect vertebrates, were detected. Contigs of coronaviruses, adenoviruses, hepeviruses, rotaviruses A and H, and parvoviruses with potential zoonotic risk were characterized in more detail. Most interestingly, in a ground stool sample of a Vespertilio murinus colony an almost complete genome of a Middle East respiratory syndrome-related coronavirus (MERS-CoV) was detected by Next generation sequencing and confirmed by PCR. In conclusion, bats in Switzerland naturally harbour many different viruses. Metagenomic analyses of non-invasive samples like ground stool may support effective surveillance and early detection of viral zoonoses. Text Vespertilio murinus PubMed Central (PMC) PLOS ONE 16 6 e0252534 |
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Research Article Hardmeier, Isabelle Aeberhard, Nadja Qi, Weihong Schoenbaechler, Katja Kraettli, Hubert Hatt, Jean-Michel Fraefel, Cornel Kubacki, Jakub Metagenomic analysis of fecal and tissue samples from 18 endemic bat species in Switzerland revealed a diverse virus composition including potentially zoonotic viruses |
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Research Article |
description |
Many recent disease outbreaks in humans had a zoonotic virus etiology. Bats in particular have been recognized as reservoirs to a large variety of viruses with the potential to cross-species transmission. In order to assess the risk of bats in Switzerland for such transmissions, we determined the virome of tissue and fecal samples of 14 native and 4 migrating bat species. In total, sequences belonging to 39 different virus families, 16 of which are known to infect vertebrates, were detected. Contigs of coronaviruses, adenoviruses, hepeviruses, rotaviruses A and H, and parvoviruses with potential zoonotic risk were characterized in more detail. Most interestingly, in a ground stool sample of a Vespertilio murinus colony an almost complete genome of a Middle East respiratory syndrome-related coronavirus (MERS-CoV) was detected by Next generation sequencing and confirmed by PCR. In conclusion, bats in Switzerland naturally harbour many different viruses. Metagenomic analyses of non-invasive samples like ground stool may support effective surveillance and early detection of viral zoonoses. |
format |
Text |
author |
Hardmeier, Isabelle Aeberhard, Nadja Qi, Weihong Schoenbaechler, Katja Kraettli, Hubert Hatt, Jean-Michel Fraefel, Cornel Kubacki, Jakub |
author_facet |
Hardmeier, Isabelle Aeberhard, Nadja Qi, Weihong Schoenbaechler, Katja Kraettli, Hubert Hatt, Jean-Michel Fraefel, Cornel Kubacki, Jakub |
author_sort |
Hardmeier, Isabelle |
title |
Metagenomic analysis of fecal and tissue samples from 18 endemic bat species in Switzerland revealed a diverse virus composition including potentially zoonotic viruses |
title_short |
Metagenomic analysis of fecal and tissue samples from 18 endemic bat species in Switzerland revealed a diverse virus composition including potentially zoonotic viruses |
title_full |
Metagenomic analysis of fecal and tissue samples from 18 endemic bat species in Switzerland revealed a diverse virus composition including potentially zoonotic viruses |
title_fullStr |
Metagenomic analysis of fecal and tissue samples from 18 endemic bat species in Switzerland revealed a diverse virus composition including potentially zoonotic viruses |
title_full_unstemmed |
Metagenomic analysis of fecal and tissue samples from 18 endemic bat species in Switzerland revealed a diverse virus composition including potentially zoonotic viruses |
title_sort |
metagenomic analysis of fecal and tissue samples from 18 endemic bat species in switzerland revealed a diverse virus composition including potentially zoonotic viruses |
publisher |
Public Library of Science |
publishDate |
2021 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8208571/ http://www.ncbi.nlm.nih.gov/pubmed/34133435 https://doi.org/10.1371/journal.pone.0252534 |
genre |
Vespertilio murinus |
genre_facet |
Vespertilio murinus |
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PLoS One |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8208571/ http://www.ncbi.nlm.nih.gov/pubmed/34133435 http://dx.doi.org/10.1371/journal.pone.0252534 |
op_rights |
© 2021 Hardmeier et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
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CC-BY |
op_doi |
https://doi.org/10.1371/journal.pone.0252534 |
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PLOS ONE |
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16 |
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