Short communication: Evaluation of charged membrane filters and buffers for concentration and recovery of infectious salmon anaemia virus in seawater
Infectious salmon anaemia virus (ISAV) is the cause of an important waterborne disease of farmed Atlantic salmon. Detection of virus in water samples may constitute an alternative method to sacrificing fish for surveillance of fish populations for the presence of ISA-virus. We aimed to evaluate diff...
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ftpubmed:oai:pubmedcentral.nih.gov:8208535 2023-05-15T15:32:24+02:00 Short communication: Evaluation of charged membrane filters and buffers for concentration and recovery of infectious salmon anaemia virus in seawater Weli, Simon Chioma Tartor, Haitham Spilsberg, Bjørn Dale, Ole Bendik Lillehaug, Atle 2021-06-16 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8208535/ http://www.ncbi.nlm.nih.gov/pubmed/34133472 https://doi.org/10.1371/journal.pone.0253297 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8208535/ http://www.ncbi.nlm.nih.gov/pubmed/34133472 http://dx.doi.org/10.1371/journal.pone.0253297 © 2021 Weli et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. CC-BY PLoS One Research Article Text 2021 ftpubmed https://doi.org/10.1371/journal.pone.0253297 2021-07-04T00:37:21Z Infectious salmon anaemia virus (ISAV) is the cause of an important waterborne disease of farmed Atlantic salmon. Detection of virus in water samples may constitute an alternative method to sacrificing fish for surveillance of fish populations for the presence of ISA-virus. We aimed to evaluate different membrane filters and buffers for concentration and recovery of ISAV in seawater, prior to molecular detection. One litre each of artificial and natural seawater was spiked with ISAV, followed by concentration with different filters and subsequent elution with different buffers. The negatively charged MF hydrophilic membrane filter, combined with NucliSENS(®) lysis buffer, presented the highest ISAV recovery percentages with 12.5 ± 1.3% by RT-qPCR and 31.7 ± 10.7% by RT-ddPCR. For the positively charged 1 MDS Zeta Plus(®) Virosorb(®) membrane filter, combined with NucliSENS(®) lysis buffer, the ISAV recovery percentages were 3.4 ± 0.1% by RT-qPCR and 10.8 ± 14.2% by RT-ddPCR. The limits of quantification (LOQ) were estimated to be 2.2 x 10(3) ISAV copies/L of natural seawater for both RT-qPCR and RT-ddPCR. The ISAV concentration method was more efficient in natural seawater. Text Atlantic salmon PubMed Central (PMC) PLOS ONE 16 6 e0253297 |
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Research Article Weli, Simon Chioma Tartor, Haitham Spilsberg, Bjørn Dale, Ole Bendik Lillehaug, Atle Short communication: Evaluation of charged membrane filters and buffers for concentration and recovery of infectious salmon anaemia virus in seawater |
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Research Article |
description |
Infectious salmon anaemia virus (ISAV) is the cause of an important waterborne disease of farmed Atlantic salmon. Detection of virus in water samples may constitute an alternative method to sacrificing fish for surveillance of fish populations for the presence of ISA-virus. We aimed to evaluate different membrane filters and buffers for concentration and recovery of ISAV in seawater, prior to molecular detection. One litre each of artificial and natural seawater was spiked with ISAV, followed by concentration with different filters and subsequent elution with different buffers. The negatively charged MF hydrophilic membrane filter, combined with NucliSENS(®) lysis buffer, presented the highest ISAV recovery percentages with 12.5 ± 1.3% by RT-qPCR and 31.7 ± 10.7% by RT-ddPCR. For the positively charged 1 MDS Zeta Plus(®) Virosorb(®) membrane filter, combined with NucliSENS(®) lysis buffer, the ISAV recovery percentages were 3.4 ± 0.1% by RT-qPCR and 10.8 ± 14.2% by RT-ddPCR. The limits of quantification (LOQ) were estimated to be 2.2 x 10(3) ISAV copies/L of natural seawater for both RT-qPCR and RT-ddPCR. The ISAV concentration method was more efficient in natural seawater. |
format |
Text |
author |
Weli, Simon Chioma Tartor, Haitham Spilsberg, Bjørn Dale, Ole Bendik Lillehaug, Atle |
author_facet |
Weli, Simon Chioma Tartor, Haitham Spilsberg, Bjørn Dale, Ole Bendik Lillehaug, Atle |
author_sort |
Weli, Simon Chioma |
title |
Short communication: Evaluation of charged membrane filters and buffers for concentration and recovery of infectious salmon anaemia virus in seawater |
title_short |
Short communication: Evaluation of charged membrane filters and buffers for concentration and recovery of infectious salmon anaemia virus in seawater |
title_full |
Short communication: Evaluation of charged membrane filters and buffers for concentration and recovery of infectious salmon anaemia virus in seawater |
title_fullStr |
Short communication: Evaluation of charged membrane filters and buffers for concentration and recovery of infectious salmon anaemia virus in seawater |
title_full_unstemmed |
Short communication: Evaluation of charged membrane filters and buffers for concentration and recovery of infectious salmon anaemia virus in seawater |
title_sort |
short communication: evaluation of charged membrane filters and buffers for concentration and recovery of infectious salmon anaemia virus in seawater |
publisher |
Public Library of Science |
publishDate |
2021 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8208535/ http://www.ncbi.nlm.nih.gov/pubmed/34133472 https://doi.org/10.1371/journal.pone.0253297 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
PLoS One |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8208535/ http://www.ncbi.nlm.nih.gov/pubmed/34133472 http://dx.doi.org/10.1371/journal.pone.0253297 |
op_rights |
© 2021 Weli et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
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CC-BY |
op_doi |
https://doi.org/10.1371/journal.pone.0253297 |
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PLOS ONE |
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16 |
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6 |
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e0253297 |
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