Evaluation of Polymeric Matrix Loaded with Melatonin for Wound Dressing
The development of scaffolds mimicking the extracellular matrix containing bioactive substances has great potential in tissue engineering and wound healing applications. This study investigates melatonin—a methoxyindole present in almost all biological systems. Melatonin is a bioregulator in terms o...
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ftpubmed:oai:pubmedcentral.nih.gov:8197906 2023-05-15T18:09:53+02:00 Evaluation of Polymeric Matrix Loaded with Melatonin for Wound Dressing Kaczmarek-Szczepańska, Beata Ostrowska, Justyna Kozłowska, Justyna Szota, Zofia Brożyna, Anna A. Dreier, Rita Reiter, Russel J. Slominski, Andrzej T. Steinbrink, Kerstin Kleszczyński, Konrad 2021-05-26 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8197906/ http://www.ncbi.nlm.nih.gov/pubmed/34073402 https://doi.org/10.3390/ijms22115658 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8197906/ http://www.ncbi.nlm.nih.gov/pubmed/34073402 http://dx.doi.org/10.3390/ijms22115658 © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). CC-BY Int J Mol Sci Article Text 2021 ftpubmed https://doi.org/10.3390/ijms22115658 2021-06-20T00:42:51Z The development of scaffolds mimicking the extracellular matrix containing bioactive substances has great potential in tissue engineering and wound healing applications. This study investigates melatonin—a methoxyindole present in almost all biological systems. Melatonin is a bioregulator in terms of its potential clinical importance for future therapies of cutaneous diseases. Mammalian skin is not only a prominent melatonin target, but also produces and rapidly metabolizes the multifunctional methoxyindole to biologically active metabolites. In our methodology, chitosan/collagen (CTS/Coll)-contained biomaterials are blended with melatonin at different doses to fabricate biomimetic hybrid scaffolds. We use rat tail tendon- and Salmo salar fish skin-derived collagens to assess biophysical and cellular properties by (i) Fourier transform infrared spectroscopy—attenuated total reflectance (FTIR–ATR), (ii) thermogravimetric analysis (TG), (iii) scanning electron microscope (SEM), and (iv) proliferation ratio of cutaneous cells in vitro. Our results indicate that melatonin itself does not negatively affect biophysical properties of melatonin-immobilized hybrid scaffolds, but it induces a pronounced elevation of cell viability within human epidermal keratinocytes (NHEK), dermal fibroblasts (NHDF), and reference melanoma cells. These results demonstrate that this indoleamine accelerates re-epithelialization. This delivery is a promising technique for additional explorations in future dermatotherapy and protective skin medicine. Text Salmo salar PubMed Central (PMC) International Journal of Molecular Sciences 22 11 5658 |
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Article Kaczmarek-Szczepańska, Beata Ostrowska, Justyna Kozłowska, Justyna Szota, Zofia Brożyna, Anna A. Dreier, Rita Reiter, Russel J. Slominski, Andrzej T. Steinbrink, Kerstin Kleszczyński, Konrad Evaluation of Polymeric Matrix Loaded with Melatonin for Wound Dressing |
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Article |
description |
The development of scaffolds mimicking the extracellular matrix containing bioactive substances has great potential in tissue engineering and wound healing applications. This study investigates melatonin—a methoxyindole present in almost all biological systems. Melatonin is a bioregulator in terms of its potential clinical importance for future therapies of cutaneous diseases. Mammalian skin is not only a prominent melatonin target, but also produces and rapidly metabolizes the multifunctional methoxyindole to biologically active metabolites. In our methodology, chitosan/collagen (CTS/Coll)-contained biomaterials are blended with melatonin at different doses to fabricate biomimetic hybrid scaffolds. We use rat tail tendon- and Salmo salar fish skin-derived collagens to assess biophysical and cellular properties by (i) Fourier transform infrared spectroscopy—attenuated total reflectance (FTIR–ATR), (ii) thermogravimetric analysis (TG), (iii) scanning electron microscope (SEM), and (iv) proliferation ratio of cutaneous cells in vitro. Our results indicate that melatonin itself does not negatively affect biophysical properties of melatonin-immobilized hybrid scaffolds, but it induces a pronounced elevation of cell viability within human epidermal keratinocytes (NHEK), dermal fibroblasts (NHDF), and reference melanoma cells. These results demonstrate that this indoleamine accelerates re-epithelialization. This delivery is a promising technique for additional explorations in future dermatotherapy and protective skin medicine. |
format |
Text |
author |
Kaczmarek-Szczepańska, Beata Ostrowska, Justyna Kozłowska, Justyna Szota, Zofia Brożyna, Anna A. Dreier, Rita Reiter, Russel J. Slominski, Andrzej T. Steinbrink, Kerstin Kleszczyński, Konrad |
author_facet |
Kaczmarek-Szczepańska, Beata Ostrowska, Justyna Kozłowska, Justyna Szota, Zofia Brożyna, Anna A. Dreier, Rita Reiter, Russel J. Slominski, Andrzej T. Steinbrink, Kerstin Kleszczyński, Konrad |
author_sort |
Kaczmarek-Szczepańska, Beata |
title |
Evaluation of Polymeric Matrix Loaded with Melatonin for Wound Dressing |
title_short |
Evaluation of Polymeric Matrix Loaded with Melatonin for Wound Dressing |
title_full |
Evaluation of Polymeric Matrix Loaded with Melatonin for Wound Dressing |
title_fullStr |
Evaluation of Polymeric Matrix Loaded with Melatonin for Wound Dressing |
title_full_unstemmed |
Evaluation of Polymeric Matrix Loaded with Melatonin for Wound Dressing |
title_sort |
evaluation of polymeric matrix loaded with melatonin for wound dressing |
publisher |
MDPI |
publishDate |
2021 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8197906/ http://www.ncbi.nlm.nih.gov/pubmed/34073402 https://doi.org/10.3390/ijms22115658 |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_source |
Int J Mol Sci |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8197906/ http://www.ncbi.nlm.nih.gov/pubmed/34073402 http://dx.doi.org/10.3390/ijms22115658 |
op_rights |
© 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
op_doi |
https://doi.org/10.3390/ijms22115658 |
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International Journal of Molecular Sciences |
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22 |
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11 |
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5658 |
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1766182564408590336 |