Subfunctionalization of Paralog Transcription Factors Contributes to Regulation of Alkaloid Pathway Branch Choice in Catharanthus roseus
Catharanthus roseus produces a diverse range of specialized metabolites of the monoterpenoid indole alkaloid (MIA) class in a heavily branched pathway. Recent great progress in identification of MIA biosynthesis genes revealed that the different pathway branch genes are expressed in a highly cell ty...
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ftpubmed:oai:pubmedcentral.nih.gov:8186833 2023-05-15T17:53:19+02:00 Subfunctionalization of Paralog Transcription Factors Contributes to Regulation of Alkaloid Pathway Branch Choice in Catharanthus roseus Colinas, Maite Pollier, Jacob Vaneechoutte, Dries Malat, Deniz G. Schweizer, Fabian De Milde, Liesbeth De Clercq, Rebecca Guedes, Joana G. Martínez-Cortés, Teresa Molina-Hidalgo, Francisco J. Sottomayor, Mariana Vandepoele, Klaas Goossens, Alain 2021-05-25 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8186833/ https://doi.org/10.3389/fpls.2021.687406 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8186833/ http://dx.doi.org/10.3389/fpls.2021.687406 Copyright © 2021 Colinas, Pollier, Vaneechoutte, Malat, Schweizer, De Milde, De Clercq, Guedes, Martínez-Cortés, Molina-Hidalgo, Sottomayor, Vandepoele and Goossens. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. CC-BY Front Plant Sci Plant Science Text 2021 ftpubmed https://doi.org/10.3389/fpls.2021.687406 2021-06-13T00:43:41Z Catharanthus roseus produces a diverse range of specialized metabolites of the monoterpenoid indole alkaloid (MIA) class in a heavily branched pathway. Recent great progress in identification of MIA biosynthesis genes revealed that the different pathway branch genes are expressed in a highly cell type- and organ-specific and stress-dependent manner. This implies a complex control by specific transcription factors (TFs), only partly revealed today. We generated and mined a comprehensive compendium of publicly available C. roseus transcriptome data for MIA pathway branch-specific TFs. Functional analysis was performed through extensive comparative gene expression analysis and profiling of over 40 MIA metabolites in the C. roseus flower petal expression system. We identified additional members of the known BIS and ORCA regulators. Further detailed study of the ORCA TFs suggests subfunctionalization of ORCA paralogs in terms of target gene-specific regulation and synergistic activity with the central jasmonate response regulator MYC2. Moreover, we identified specific amino acid residues within the ORCA DNA-binding domains that contribute to the differential regulation of some MIA pathway branches. Our results advance our understanding of TF paralog specificity for which, despite the common occurrence of closely related paralogs in many species, comparative studies are scarce. Text Orca PubMed Central (PMC) Frontiers in Plant Science 12 |
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Plant Science |
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Plant Science Colinas, Maite Pollier, Jacob Vaneechoutte, Dries Malat, Deniz G. Schweizer, Fabian De Milde, Liesbeth De Clercq, Rebecca Guedes, Joana G. Martínez-Cortés, Teresa Molina-Hidalgo, Francisco J. Sottomayor, Mariana Vandepoele, Klaas Goossens, Alain Subfunctionalization of Paralog Transcription Factors Contributes to Regulation of Alkaloid Pathway Branch Choice in Catharanthus roseus |
topic_facet |
Plant Science |
description |
Catharanthus roseus produces a diverse range of specialized metabolites of the monoterpenoid indole alkaloid (MIA) class in a heavily branched pathway. Recent great progress in identification of MIA biosynthesis genes revealed that the different pathway branch genes are expressed in a highly cell type- and organ-specific and stress-dependent manner. This implies a complex control by specific transcription factors (TFs), only partly revealed today. We generated and mined a comprehensive compendium of publicly available C. roseus transcriptome data for MIA pathway branch-specific TFs. Functional analysis was performed through extensive comparative gene expression analysis and profiling of over 40 MIA metabolites in the C. roseus flower petal expression system. We identified additional members of the known BIS and ORCA regulators. Further detailed study of the ORCA TFs suggests subfunctionalization of ORCA paralogs in terms of target gene-specific regulation and synergistic activity with the central jasmonate response regulator MYC2. Moreover, we identified specific amino acid residues within the ORCA DNA-binding domains that contribute to the differential regulation of some MIA pathway branches. Our results advance our understanding of TF paralog specificity for which, despite the common occurrence of closely related paralogs in many species, comparative studies are scarce. |
format |
Text |
author |
Colinas, Maite Pollier, Jacob Vaneechoutte, Dries Malat, Deniz G. Schweizer, Fabian De Milde, Liesbeth De Clercq, Rebecca Guedes, Joana G. Martínez-Cortés, Teresa Molina-Hidalgo, Francisco J. Sottomayor, Mariana Vandepoele, Klaas Goossens, Alain |
author_facet |
Colinas, Maite Pollier, Jacob Vaneechoutte, Dries Malat, Deniz G. Schweizer, Fabian De Milde, Liesbeth De Clercq, Rebecca Guedes, Joana G. Martínez-Cortés, Teresa Molina-Hidalgo, Francisco J. Sottomayor, Mariana Vandepoele, Klaas Goossens, Alain |
author_sort |
Colinas, Maite |
title |
Subfunctionalization of Paralog Transcription Factors Contributes to Regulation of Alkaloid Pathway Branch Choice in Catharanthus roseus |
title_short |
Subfunctionalization of Paralog Transcription Factors Contributes to Regulation of Alkaloid Pathway Branch Choice in Catharanthus roseus |
title_full |
Subfunctionalization of Paralog Transcription Factors Contributes to Regulation of Alkaloid Pathway Branch Choice in Catharanthus roseus |
title_fullStr |
Subfunctionalization of Paralog Transcription Factors Contributes to Regulation of Alkaloid Pathway Branch Choice in Catharanthus roseus |
title_full_unstemmed |
Subfunctionalization of Paralog Transcription Factors Contributes to Regulation of Alkaloid Pathway Branch Choice in Catharanthus roseus |
title_sort |
subfunctionalization of paralog transcription factors contributes to regulation of alkaloid pathway branch choice in catharanthus roseus |
publisher |
Frontiers Media S.A. |
publishDate |
2021 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8186833/ https://doi.org/10.3389/fpls.2021.687406 |
genre |
Orca |
genre_facet |
Orca |
op_source |
Front Plant Sci |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8186833/ http://dx.doi.org/10.3389/fpls.2021.687406 |
op_rights |
Copyright © 2021 Colinas, Pollier, Vaneechoutte, Malat, Schweizer, De Milde, De Clercq, Guedes, Martínez-Cortés, Molina-Hidalgo, Sottomayor, Vandepoele and Goossens. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fpls.2021.687406 |
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Frontiers in Plant Science |
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12 |
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1766161022699175936 |