Interferon Gamma Induces the Increase of Cell-Surface Markers (CD80/86, CD83 and MHC-II) in Splenocytes From Atlantic Salmon
Type II interferon gamma (IFNγ) is a pleiotropic cytokine capable of modulating the innate and adaptive immune responses which has been widely characterized in several teleost families. In fish, IFNγ stimulates the expression of cytokines and chemokines associated with the pro-inflammatory response...
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ftpubmed:oai:pubmedcentral.nih.gov:8155612 2023-05-15T15:30:56+02:00 Interferon Gamma Induces the Increase of Cell-Surface Markers (CD80/86, CD83 and MHC-II) in Splenocytes From Atlantic Salmon Morales-Lange, Byron Ramírez-Cepeda, Felipe Schmitt, Paulina Guzmán, Fanny Lagos, Leidy Øverland, Margareth Wong-Benito, Valentina Imarai, Mónica Fuentes, Derie Boltaña, Sebastián Alcaíno, Javier Soto, Carlos Mercado, Luis 2021-05-13 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155612/ https://doi.org/10.3389/fimmu.2021.666356 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155612/ http://dx.doi.org/10.3389/fimmu.2021.666356 Copyright © 2021 Morales-Lange, Ramírez-Cepeda, Schmitt, Guzmán, Lagos, Øverland, Wong-Benito, Imarai, Fuentes, Boltaña, Alcaíno, Soto and Mercado https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. CC-BY Front Immunol Immunology Text 2021 ftpubmed https://doi.org/10.3389/fimmu.2021.666356 2021-05-30T01:07:12Z Type II interferon gamma (IFNγ) is a pleiotropic cytokine capable of modulating the innate and adaptive immune responses which has been widely characterized in several teleost families. In fish, IFNγ stimulates the expression of cytokines and chemokines associated with the pro-inflammatory response and enhances the production of nitrogen and oxygen reactive species in phagocytic cells. This work studied the effect of IFNγ on the expression of cell-surface markers on splenocytes of Atlantic salmon (Salmo salar). In vitro results showed that subpopulations of mononuclear splenocytes cultured for 15 days were capable of increasing gene expression and protein availability of cell-surface markers such as CD80/86, CD83 and MHC II, after being stimulated with recombinant IFNγ. These results were observed for subpopulations with characteristics associated with monocytes (51%), and features that could be related to lymphocytes (46.3%). In addition, a decrease in the expression of zbtb46 was detected in IFNγ-stimulated splenocytes. Finally, the expression of IFNγ and cell-surface markers was assessed in Atlantic salmon under field conditions. In vivo results showed that the expression of ifnγ increased simultaneously with the up-regulation of cd80/86, cd83 and mhcii during a natural outbreak of Piscirickettsia salmonis. Overall, the results obtained in this study allow us to propose IFNγ as a candidate molecule to stimulate the phenotypic progression of a small population of immune cells, which will increase antigen presenting cells markers. Thereby, modulatory strategies using IFNγ may generate a robust and coordinated immune response in fish against pathogens that affect aquaculture. Text Atlantic salmon Salmo salar PubMed Central (PMC) Frontiers in Immunology 12 |
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English |
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Immunology |
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Immunology Morales-Lange, Byron Ramírez-Cepeda, Felipe Schmitt, Paulina Guzmán, Fanny Lagos, Leidy Øverland, Margareth Wong-Benito, Valentina Imarai, Mónica Fuentes, Derie Boltaña, Sebastián Alcaíno, Javier Soto, Carlos Mercado, Luis Interferon Gamma Induces the Increase of Cell-Surface Markers (CD80/86, CD83 and MHC-II) in Splenocytes From Atlantic Salmon |
topic_facet |
Immunology |
description |
Type II interferon gamma (IFNγ) is a pleiotropic cytokine capable of modulating the innate and adaptive immune responses which has been widely characterized in several teleost families. In fish, IFNγ stimulates the expression of cytokines and chemokines associated with the pro-inflammatory response and enhances the production of nitrogen and oxygen reactive species in phagocytic cells. This work studied the effect of IFNγ on the expression of cell-surface markers on splenocytes of Atlantic salmon (Salmo salar). In vitro results showed that subpopulations of mononuclear splenocytes cultured for 15 days were capable of increasing gene expression and protein availability of cell-surface markers such as CD80/86, CD83 and MHC II, after being stimulated with recombinant IFNγ. These results were observed for subpopulations with characteristics associated with monocytes (51%), and features that could be related to lymphocytes (46.3%). In addition, a decrease in the expression of zbtb46 was detected in IFNγ-stimulated splenocytes. Finally, the expression of IFNγ and cell-surface markers was assessed in Atlantic salmon under field conditions. In vivo results showed that the expression of ifnγ increased simultaneously with the up-regulation of cd80/86, cd83 and mhcii during a natural outbreak of Piscirickettsia salmonis. Overall, the results obtained in this study allow us to propose IFNγ as a candidate molecule to stimulate the phenotypic progression of a small population of immune cells, which will increase antigen presenting cells markers. Thereby, modulatory strategies using IFNγ may generate a robust and coordinated immune response in fish against pathogens that affect aquaculture. |
format |
Text |
author |
Morales-Lange, Byron Ramírez-Cepeda, Felipe Schmitt, Paulina Guzmán, Fanny Lagos, Leidy Øverland, Margareth Wong-Benito, Valentina Imarai, Mónica Fuentes, Derie Boltaña, Sebastián Alcaíno, Javier Soto, Carlos Mercado, Luis |
author_facet |
Morales-Lange, Byron Ramírez-Cepeda, Felipe Schmitt, Paulina Guzmán, Fanny Lagos, Leidy Øverland, Margareth Wong-Benito, Valentina Imarai, Mónica Fuentes, Derie Boltaña, Sebastián Alcaíno, Javier Soto, Carlos Mercado, Luis |
author_sort |
Morales-Lange, Byron |
title |
Interferon Gamma Induces the Increase of Cell-Surface Markers (CD80/86, CD83 and MHC-II) in Splenocytes From Atlantic Salmon |
title_short |
Interferon Gamma Induces the Increase of Cell-Surface Markers (CD80/86, CD83 and MHC-II) in Splenocytes From Atlantic Salmon |
title_full |
Interferon Gamma Induces the Increase of Cell-Surface Markers (CD80/86, CD83 and MHC-II) in Splenocytes From Atlantic Salmon |
title_fullStr |
Interferon Gamma Induces the Increase of Cell-Surface Markers (CD80/86, CD83 and MHC-II) in Splenocytes From Atlantic Salmon |
title_full_unstemmed |
Interferon Gamma Induces the Increase of Cell-Surface Markers (CD80/86, CD83 and MHC-II) in Splenocytes From Atlantic Salmon |
title_sort |
interferon gamma induces the increase of cell-surface markers (cd80/86, cd83 and mhc-ii) in splenocytes from atlantic salmon |
publisher |
Frontiers Media S.A. |
publishDate |
2021 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155612/ https://doi.org/10.3389/fimmu.2021.666356 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Front Immunol |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155612/ http://dx.doi.org/10.3389/fimmu.2021.666356 |
op_rights |
Copyright © 2021 Morales-Lange, Ramírez-Cepeda, Schmitt, Guzmán, Lagos, Øverland, Wong-Benito, Imarai, Fuentes, Boltaña, Alcaíno, Soto and Mercado https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fimmu.2021.666356 |
container_title |
Frontiers in Immunology |
container_volume |
12 |
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1766361405321117696 |