Agglutination Activity of Fasciola gigantica DM9-1, a Mannose-Binding Lectin
The DM9 domain is a protein unit of 60–75 amino acids that has been first detected in the fruit fly Drosophila as a repeated motif of unknown function. Recent research on proteins carrying DM9 domains in the mosquito Anopheles gambiae and the oyster Crassostrea gigas indicated an association with th...
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ftpubmed:oai:pubmedcentral.nih.gov:8106982 2023-05-15T15:57:47+02:00 Agglutination Activity of Fasciola gigantica DM9-1, a Mannose-Binding Lectin Phadungsil, Wansika Grams, Rudi 2021-04 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106982/ http://www.ncbi.nlm.nih.gov/pubmed/33951774 https://doi.org/10.3347/kjp.2021.59.2.173 en eng The Korean Society for Parasitology and Tropical Medicine http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106982/ http://www.ncbi.nlm.nih.gov/pubmed/33951774 http://dx.doi.org/10.3347/kjp.2021.59.2.173 © 2021, Korean Society for Parasitology and Tropical Medicine https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. CC-BY-NC Korean J Parasitol Brief Communication Text 2021 ftpubmed https://doi.org/10.3347/kjp.2021.59.2.173 2021-05-23T00:25:47Z The DM9 domain is a protein unit of 60–75 amino acids that has been first detected in the fruit fly Drosophila as a repeated motif of unknown function. Recent research on proteins carrying DM9 domains in the mosquito Anopheles gambiae and the oyster Crassostrea gigas indicated an association with the uptake of microbial organisms. Likewise, in the trematode Fasciola gigantica DM9-1 showed intracellular relocalization following microbial, heat and drug stress. In the present research, we show that FgDM9-1 is a lectin with a novel mannose-binding site that has been recently described for the protein CGL1 of Crassostrea gigas. This property allowed FgDM9-1 to agglutinate gram-positive and -negative bacteria with appropriate cell surface glycosylation patterns. Furthermore, FgDM9-1 caused hemagglutination across all ABO blood group phenotypes. It is speculated that the parenchymal located FgDM9-1 has a role in cellular processes that involve the transport of mannose-carrying molecules in the parenchymal cells of the parasite. Text Crassostrea gigas PubMed Central (PMC) The Korean Journal of Parasitology 59 2 173 178 |
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English |
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Brief Communication |
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Brief Communication Phadungsil, Wansika Grams, Rudi Agglutination Activity of Fasciola gigantica DM9-1, a Mannose-Binding Lectin |
topic_facet |
Brief Communication |
description |
The DM9 domain is a protein unit of 60–75 amino acids that has been first detected in the fruit fly Drosophila as a repeated motif of unknown function. Recent research on proteins carrying DM9 domains in the mosquito Anopheles gambiae and the oyster Crassostrea gigas indicated an association with the uptake of microbial organisms. Likewise, in the trematode Fasciola gigantica DM9-1 showed intracellular relocalization following microbial, heat and drug stress. In the present research, we show that FgDM9-1 is a lectin with a novel mannose-binding site that has been recently described for the protein CGL1 of Crassostrea gigas. This property allowed FgDM9-1 to agglutinate gram-positive and -negative bacteria with appropriate cell surface glycosylation patterns. Furthermore, FgDM9-1 caused hemagglutination across all ABO blood group phenotypes. It is speculated that the parenchymal located FgDM9-1 has a role in cellular processes that involve the transport of mannose-carrying molecules in the parenchymal cells of the parasite. |
format |
Text |
author |
Phadungsil, Wansika Grams, Rudi |
author_facet |
Phadungsil, Wansika Grams, Rudi |
author_sort |
Phadungsil, Wansika |
title |
Agglutination Activity of Fasciola gigantica DM9-1, a Mannose-Binding Lectin |
title_short |
Agglutination Activity of Fasciola gigantica DM9-1, a Mannose-Binding Lectin |
title_full |
Agglutination Activity of Fasciola gigantica DM9-1, a Mannose-Binding Lectin |
title_fullStr |
Agglutination Activity of Fasciola gigantica DM9-1, a Mannose-Binding Lectin |
title_full_unstemmed |
Agglutination Activity of Fasciola gigantica DM9-1, a Mannose-Binding Lectin |
title_sort |
agglutination activity of fasciola gigantica dm9-1, a mannose-binding lectin |
publisher |
The Korean Society for Parasitology and Tropical Medicine |
publishDate |
2021 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106982/ http://www.ncbi.nlm.nih.gov/pubmed/33951774 https://doi.org/10.3347/kjp.2021.59.2.173 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Korean J Parasitol |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106982/ http://www.ncbi.nlm.nih.gov/pubmed/33951774 http://dx.doi.org/10.3347/kjp.2021.59.2.173 |
op_rights |
© 2021, Korean Society for Parasitology and Tropical Medicine https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
op_rightsnorm |
CC-BY-NC |
op_doi |
https://doi.org/10.3347/kjp.2021.59.2.173 |
container_title |
The Korean Journal of Parasitology |
container_volume |
59 |
container_issue |
2 |
container_start_page |
173 |
op_container_end_page |
178 |
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1766393489992450048 |