Insulin-Like Growth Factors-1 Receptor (IGF-1R) Expression and the Phosphorylation of Endogenous Substrates Lead to Maturation of the Pacific oyster, Crassostrea gigas
This study investigated the IGF-1 signal in specific tissues using Pacific oysters artificially matured via water temperature elevation. Pacific oysters were subjected to water temperature elevation from March to June, and 20 were randomly sampled each month. The condition index (CI) and tissue weig...
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Korean Society of Developmental Biology
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ftpubmed:oai:pubmedcentral.nih.gov:8087256 2023-05-15T15:58:41+02:00 Insulin-Like Growth Factors-1 Receptor (IGF-1R) Expression and the Phosphorylation of Endogenous Substrates Lead to Maturation of the Pacific oyster, Crassostrea gigas Park, Su-Jin Choi, Youn Hee 2021-03 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8087256/ http://www.ncbi.nlm.nih.gov/pubmed/33977177 https://doi.org/10.12717/DR.2021.25.1.67 en eng Korean Society of Developmental Biology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8087256/ http://www.ncbi.nlm.nih.gov/pubmed/33977177 http://dx.doi.org/10.12717/DR.2021.25.1.67 © Copyright 2021 The Korean Society of Developmental Biology https://creativecommons.org/licenses/by-nc/4.0/This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creative-commons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. CC-BY-NC Dev Reprod Short Communication Text 2021 ftpubmed https://doi.org/10.12717/DR.2021.25.1.67 2021-05-16T00:28:10Z This study investigated the IGF-1 signal in specific tissues using Pacific oysters artificially matured via water temperature elevation. Pacific oysters were subjected to water temperature elevation from March to June, and 20 were randomly sampled each month. The condition index (CI) and tissue weight rate (TWR) were examined by measuring shell length, shell height, shell width, and soft tissue weight. The IGF-1 signal in tissues (adductor muscle, digestive glands, gills, labial palps, mantle edges, and gonads) was analyzed by sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. From April to June, the TWR of females and males increased from 19.1±2.9 to 21.0±3.6 and 18.2±2.0 to 19.2±2.5, respectively, while the CI remained the same. The IGF-1 signal in each tissue differed. IGF-1 was expressed in the adductor muscle, while tyrosine was expressed in all tissues. The phosphor (p)-ERK and p-AKT activities were high in the adductor muscle, mantle edge, and gonads. IGF-1 signaling affected the growth and maturity of the Pacific oysters examined. Text Crassostrea gigas Pacific oyster PubMed Central (PMC) Pacific Development & Reproduction 25 1 67 72 |
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Short Communication |
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Short Communication Park, Su-Jin Choi, Youn Hee Insulin-Like Growth Factors-1 Receptor (IGF-1R) Expression and the Phosphorylation of Endogenous Substrates Lead to Maturation of the Pacific oyster, Crassostrea gigas |
topic_facet |
Short Communication |
description |
This study investigated the IGF-1 signal in specific tissues using Pacific oysters artificially matured via water temperature elevation. Pacific oysters were subjected to water temperature elevation from March to June, and 20 were randomly sampled each month. The condition index (CI) and tissue weight rate (TWR) were examined by measuring shell length, shell height, shell width, and soft tissue weight. The IGF-1 signal in tissues (adductor muscle, digestive glands, gills, labial palps, mantle edges, and gonads) was analyzed by sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. From April to June, the TWR of females and males increased from 19.1±2.9 to 21.0±3.6 and 18.2±2.0 to 19.2±2.5, respectively, while the CI remained the same. The IGF-1 signal in each tissue differed. IGF-1 was expressed in the adductor muscle, while tyrosine was expressed in all tissues. The phosphor (p)-ERK and p-AKT activities were high in the adductor muscle, mantle edge, and gonads. IGF-1 signaling affected the growth and maturity of the Pacific oysters examined. |
format |
Text |
author |
Park, Su-Jin Choi, Youn Hee |
author_facet |
Park, Su-Jin Choi, Youn Hee |
author_sort |
Park, Su-Jin |
title |
Insulin-Like Growth Factors-1 Receptor (IGF-1R) Expression and the Phosphorylation of Endogenous Substrates Lead to Maturation of the Pacific oyster, Crassostrea gigas |
title_short |
Insulin-Like Growth Factors-1 Receptor (IGF-1R) Expression and the Phosphorylation of Endogenous Substrates Lead to Maturation of the Pacific oyster, Crassostrea gigas |
title_full |
Insulin-Like Growth Factors-1 Receptor (IGF-1R) Expression and the Phosphorylation of Endogenous Substrates Lead to Maturation of the Pacific oyster, Crassostrea gigas |
title_fullStr |
Insulin-Like Growth Factors-1 Receptor (IGF-1R) Expression and the Phosphorylation of Endogenous Substrates Lead to Maturation of the Pacific oyster, Crassostrea gigas |
title_full_unstemmed |
Insulin-Like Growth Factors-1 Receptor (IGF-1R) Expression and the Phosphorylation of Endogenous Substrates Lead to Maturation of the Pacific oyster, Crassostrea gigas |
title_sort |
insulin-like growth factors-1 receptor (igf-1r) expression and the phosphorylation of endogenous substrates lead to maturation of the pacific oyster, crassostrea gigas |
publisher |
Korean Society of Developmental Biology |
publishDate |
2021 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8087256/ http://www.ncbi.nlm.nih.gov/pubmed/33977177 https://doi.org/10.12717/DR.2021.25.1.67 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Dev Reprod |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8087256/ http://www.ncbi.nlm.nih.gov/pubmed/33977177 http://dx.doi.org/10.12717/DR.2021.25.1.67 |
op_rights |
© Copyright 2021 The Korean Society of Developmental Biology https://creativecommons.org/licenses/by-nc/4.0/This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creative-commons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
op_rightsnorm |
CC-BY-NC |
op_doi |
https://doi.org/10.12717/DR.2021.25.1.67 |
container_title |
Development & Reproduction |
container_volume |
25 |
container_issue |
1 |
container_start_page |
67 |
op_container_end_page |
72 |
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1766394452689027072 |