Genetic Characterization of a Neurovirulent West Nile Virus Variant Associated with a Fatal Great Grey Owl Infection
This study reports on a fatal case of a captive great grey owl infected with the West Nile virus (WNV) in the zoological garden Košice, eastern Slovakia (Central Europe). The tissue samples of the dead owl were used for virus isolation and genetic characterization. The novel isolate is genetically c...
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ftpubmed:oai:pubmedcentral.nih.gov:8073349 2023-05-15T16:23:00+02:00 Genetic Characterization of a Neurovirulent West Nile Virus Variant Associated with a Fatal Great Grey Owl Infection Peňazziová, Katarína Korytár, Ľuboš Pastorek, Patrik Pistl, Juraj Rusňáková, Diana Szemes, Tomáš Čabanová, Viktória Ličková, Martina Boršová, Kristína Klempa, Boris Csank, Tomáš 2021-04-17 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8073349/ https://doi.org/10.3390/v13040699 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8073349/ http://dx.doi.org/10.3390/v13040699 © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). CC-BY Viruses Communication Text 2021 ftpubmed https://doi.org/10.3390/v13040699 2021-05-02T00:52:40Z This study reports on a fatal case of a captive great grey owl infected with the West Nile virus (WNV) in the zoological garden Košice, eastern Slovakia (Central Europe). The tissue samples of the dead owl were used for virus isolation and genetic characterization. The novel isolate is genetically closer to Hungarian, Greek, and Bulgarian strains from the central/southern European clade of lineage 2 than to the strains previously isolated in Slovakia. Interestingly, it carries NS3-249P, a molecular virulence determinant associated with higher neurovirulence, which has not previously been observed in Slovakia. Subsequent serological investigation of the captive owls revealed additional seropositive animals, indicating local WNV transmission. Although no WNV-positive mosquitoes were found, the presence of the WNV principal vector Culex pipiens complex together with the described fatal case and further serological findings indicate an endemic focus of bird-neurovirulent WNV variant in the area. Text great grey owl PubMed Central (PMC) Viruses 13 4 699 |
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PubMed Central (PMC) |
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English |
topic |
Communication |
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Communication Peňazziová, Katarína Korytár, Ľuboš Pastorek, Patrik Pistl, Juraj Rusňáková, Diana Szemes, Tomáš Čabanová, Viktória Ličková, Martina Boršová, Kristína Klempa, Boris Csank, Tomáš Genetic Characterization of a Neurovirulent West Nile Virus Variant Associated with a Fatal Great Grey Owl Infection |
topic_facet |
Communication |
description |
This study reports on a fatal case of a captive great grey owl infected with the West Nile virus (WNV) in the zoological garden Košice, eastern Slovakia (Central Europe). The tissue samples of the dead owl were used for virus isolation and genetic characterization. The novel isolate is genetically closer to Hungarian, Greek, and Bulgarian strains from the central/southern European clade of lineage 2 than to the strains previously isolated in Slovakia. Interestingly, it carries NS3-249P, a molecular virulence determinant associated with higher neurovirulence, which has not previously been observed in Slovakia. Subsequent serological investigation of the captive owls revealed additional seropositive animals, indicating local WNV transmission. Although no WNV-positive mosquitoes were found, the presence of the WNV principal vector Culex pipiens complex together with the described fatal case and further serological findings indicate an endemic focus of bird-neurovirulent WNV variant in the area. |
format |
Text |
author |
Peňazziová, Katarína Korytár, Ľuboš Pastorek, Patrik Pistl, Juraj Rusňáková, Diana Szemes, Tomáš Čabanová, Viktória Ličková, Martina Boršová, Kristína Klempa, Boris Csank, Tomáš |
author_facet |
Peňazziová, Katarína Korytár, Ľuboš Pastorek, Patrik Pistl, Juraj Rusňáková, Diana Szemes, Tomáš Čabanová, Viktória Ličková, Martina Boršová, Kristína Klempa, Boris Csank, Tomáš |
author_sort |
Peňazziová, Katarína |
title |
Genetic Characterization of a Neurovirulent West Nile Virus Variant Associated with a Fatal Great Grey Owl Infection |
title_short |
Genetic Characterization of a Neurovirulent West Nile Virus Variant Associated with a Fatal Great Grey Owl Infection |
title_full |
Genetic Characterization of a Neurovirulent West Nile Virus Variant Associated with a Fatal Great Grey Owl Infection |
title_fullStr |
Genetic Characterization of a Neurovirulent West Nile Virus Variant Associated with a Fatal Great Grey Owl Infection |
title_full_unstemmed |
Genetic Characterization of a Neurovirulent West Nile Virus Variant Associated with a Fatal Great Grey Owl Infection |
title_sort |
genetic characterization of a neurovirulent west nile virus variant associated with a fatal great grey owl infection |
publisher |
MDPI |
publishDate |
2021 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8073349/ https://doi.org/10.3390/v13040699 |
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great grey owl |
genre_facet |
great grey owl |
op_source |
Viruses |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8073349/ http://dx.doi.org/10.3390/v13040699 |
op_rights |
© 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
op_doi |
https://doi.org/10.3390/v13040699 |
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Viruses |
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