Chimeric Protein IPath(®) with Chelating Activity Improves Atlantic Salmon’s Immunity against Infectious Diseases
Infection processes displayed by pathogens require the acquisition of essential inorganic nutrients and trace elements from the host to survive and proliferate. Without a doubt, iron is a crucial trace metal for all living organisms and also a pivotal component in the host–parasite interactions. In...
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ftpubmed:oai:pubmedcentral.nih.gov:8068967 2023-05-15T15:31:50+02:00 Chimeric Protein IPath(®) with Chelating Activity Improves Atlantic Salmon’s Immunity against Infectious Diseases Valenzuela-Muñoz, Valentina Benavente, Bárbara P. Casuso, Antonio Leal, Yeny Gallardo-Escárate, Cristian 2021-04-09 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8068967/ https://doi.org/10.3390/vaccines9040361 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8068967/ http://dx.doi.org/10.3390/vaccines9040361 © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). CC-BY Vaccines (Basel) Article Text 2021 ftpubmed https://doi.org/10.3390/vaccines9040361 2021-05-02T00:41:02Z Infection processes displayed by pathogens require the acquisition of essential inorganic nutrients and trace elements from the host to survive and proliferate. Without a doubt, iron is a crucial trace metal for all living organisms and also a pivotal component in the host–parasite interactions. In particular, the host reduces the iron available to face the infectious disease, increasing iron transport proteins’ expression and activating the heme synthesis and degradation pathways. Moreover, recent findings have suggested that iron metabolism modulation in fish promotes the immune response by reducing cellular iron toxicity. We hypothesized that recombinant proteins related to iron metabolism could modulate the fish’s immune system through iron metabolism and iron-responsive genes. Here a chimeric iron transport protein (IPath(®)) was bioinformatically designed and then expressed in a recombinant bacterial system. The IPath(®) protein showed a significant chelating activity under in vitro conditions and biological activity. Taking this evidence, a vaccine candidate based on IPath(®) was evaluated in Atlantic salmon challenged with three different fish pathogens. Experimental trials were conducted using two fish groups: one immunized with IPath(®) and another injected with adjutant as the control group. After 400 accumulated thermal units (ATUs), two different infection trials were performed. In the first one, fish were infected with the bacterium Aeromonas salmonicida, and in a second trial, fish were exposed to the ectoparasite Caligus rogercresseyi and subsequently infected with the intracellular bacterium Piscirickettsia salmonis. Fish immunized with IPath(®) showed a significant delay in the mortality curve in response to A. salmonicida and P. salmonis infections. However, no significant differences between infected and control fish groups were observed at the end of the experiment. Notably, sea lice burden reduction was observed in vaccinated Atlantic salmon. Transcriptional analysis evidenced a high ... Text Atlantic salmon PubMed Central (PMC) Vaccines 9 4 361 |
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Article Valenzuela-Muñoz, Valentina Benavente, Bárbara P. Casuso, Antonio Leal, Yeny Gallardo-Escárate, Cristian Chimeric Protein IPath(®) with Chelating Activity Improves Atlantic Salmon’s Immunity against Infectious Diseases |
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Infection processes displayed by pathogens require the acquisition of essential inorganic nutrients and trace elements from the host to survive and proliferate. Without a doubt, iron is a crucial trace metal for all living organisms and also a pivotal component in the host–parasite interactions. In particular, the host reduces the iron available to face the infectious disease, increasing iron transport proteins’ expression and activating the heme synthesis and degradation pathways. Moreover, recent findings have suggested that iron metabolism modulation in fish promotes the immune response by reducing cellular iron toxicity. We hypothesized that recombinant proteins related to iron metabolism could modulate the fish’s immune system through iron metabolism and iron-responsive genes. Here a chimeric iron transport protein (IPath(®)) was bioinformatically designed and then expressed in a recombinant bacterial system. The IPath(®) protein showed a significant chelating activity under in vitro conditions and biological activity. Taking this evidence, a vaccine candidate based on IPath(®) was evaluated in Atlantic salmon challenged with three different fish pathogens. Experimental trials were conducted using two fish groups: one immunized with IPath(®) and another injected with adjutant as the control group. After 400 accumulated thermal units (ATUs), two different infection trials were performed. In the first one, fish were infected with the bacterium Aeromonas salmonicida, and in a second trial, fish were exposed to the ectoparasite Caligus rogercresseyi and subsequently infected with the intracellular bacterium Piscirickettsia salmonis. Fish immunized with IPath(®) showed a significant delay in the mortality curve in response to A. salmonicida and P. salmonis infections. However, no significant differences between infected and control fish groups were observed at the end of the experiment. Notably, sea lice burden reduction was observed in vaccinated Atlantic salmon. Transcriptional analysis evidenced a high ... |
format |
Text |
author |
Valenzuela-Muñoz, Valentina Benavente, Bárbara P. Casuso, Antonio Leal, Yeny Gallardo-Escárate, Cristian |
author_facet |
Valenzuela-Muñoz, Valentina Benavente, Bárbara P. Casuso, Antonio Leal, Yeny Gallardo-Escárate, Cristian |
author_sort |
Valenzuela-Muñoz, Valentina |
title |
Chimeric Protein IPath(®) with Chelating Activity Improves Atlantic Salmon’s Immunity against Infectious Diseases |
title_short |
Chimeric Protein IPath(®) with Chelating Activity Improves Atlantic Salmon’s Immunity against Infectious Diseases |
title_full |
Chimeric Protein IPath(®) with Chelating Activity Improves Atlantic Salmon’s Immunity against Infectious Diseases |
title_fullStr |
Chimeric Protein IPath(®) with Chelating Activity Improves Atlantic Salmon’s Immunity against Infectious Diseases |
title_full_unstemmed |
Chimeric Protein IPath(®) with Chelating Activity Improves Atlantic Salmon’s Immunity against Infectious Diseases |
title_sort |
chimeric protein ipath(®) with chelating activity improves atlantic salmon’s immunity against infectious diseases |
publisher |
MDPI |
publishDate |
2021 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8068967/ https://doi.org/10.3390/vaccines9040361 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Vaccines (Basel) |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8068967/ http://dx.doi.org/10.3390/vaccines9040361 |
op_rights |
© 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/vaccines9040361 |
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Vaccines |
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9 |
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4 |
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361 |
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1766362346971725824 |