Molecular Characterization of Retinoic Acid Receptor CgRAR in Pacific Oyster (Crassostrea gigas)
Retinoic acid (RA) signaling pathways mediated by RA receptors (RARs) are essential for many physiological processes such as organ development, regeneration, and differentiation in animals. Recent studies reveal that RARs identified in several mollusks, including Pacific oyster Crassostrea gigas, ha...
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ftpubmed:oai:pubmedcentral.nih.gov:8060629 2023-05-15T15:57:57+02:00 Molecular Characterization of Retinoic Acid Receptor CgRAR in Pacific Oyster (Crassostrea gigas) Jin, Kaidi Jin, Qianqian Cai, Zhongqiang Huang, Baoyu Wei, Lei Zhang, Meiwei Guo, Wen Liu, Yaqiong Wang, Xiaotong 2021-04-08 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8060629/ https://doi.org/10.3389/fphys.2021.666842 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8060629/ http://dx.doi.org/10.3389/fphys.2021.666842 Copyright © 2021 Jin, Jin, Cai, Huang, Wei, Zhang, Guo, Liu and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. CC-BY Front Physiol Physiology Text 2021 ftpubmed https://doi.org/10.3389/fphys.2021.666842 2021-04-25T00:42:33Z Retinoic acid (RA) signaling pathways mediated by RA receptors (RARs) are essential for many physiological processes such as organ development, regeneration, and differentiation in animals. Recent studies reveal that RARs identified in several mollusks, including Pacific oyster Crassostrea gigas, have a different function mechanism compared with that in chordates. In this report, we identified the molecular characteristics of CgRAR to further explore the mechanism of RAR in mollusks. RT-qPCR analysis shows that CgRAR has a higher expression level in the hemocytes and gonads, indicating that CgRAR may play roles in the processes of development and metabolism. The mRNA expression level of both CgRAR and CgRXR was analyzed by RT-qPCR after injection with RA. The elevated expression of CgRAR and CgRXR was detected upon all-trans-RA (ATRA) exposure. Finally, according to the results of Yeast Two-Hybrid assay and co-immunoprecipitation analysis, CgRAR and CgRXR can interact with each other through the C-terminal region. Taken together, our results suggest that CgRAR shows a higher expression level in gonads and hemocytes. ATRA exposure up-regulates the expression of CgRAR and CgRXR. Besides, CgRAR can interact with CgRXR to form a heterodimer complex. Text Crassostrea gigas Pacific oyster PubMed Central (PMC) Pacific Frontiers in Physiology 12 |
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Physiology Jin, Kaidi Jin, Qianqian Cai, Zhongqiang Huang, Baoyu Wei, Lei Zhang, Meiwei Guo, Wen Liu, Yaqiong Wang, Xiaotong Molecular Characterization of Retinoic Acid Receptor CgRAR in Pacific Oyster (Crassostrea gigas) |
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Physiology |
description |
Retinoic acid (RA) signaling pathways mediated by RA receptors (RARs) are essential for many physiological processes such as organ development, regeneration, and differentiation in animals. Recent studies reveal that RARs identified in several mollusks, including Pacific oyster Crassostrea gigas, have a different function mechanism compared with that in chordates. In this report, we identified the molecular characteristics of CgRAR to further explore the mechanism of RAR in mollusks. RT-qPCR analysis shows that CgRAR has a higher expression level in the hemocytes and gonads, indicating that CgRAR may play roles in the processes of development and metabolism. The mRNA expression level of both CgRAR and CgRXR was analyzed by RT-qPCR after injection with RA. The elevated expression of CgRAR and CgRXR was detected upon all-trans-RA (ATRA) exposure. Finally, according to the results of Yeast Two-Hybrid assay and co-immunoprecipitation analysis, CgRAR and CgRXR can interact with each other through the C-terminal region. Taken together, our results suggest that CgRAR shows a higher expression level in gonads and hemocytes. ATRA exposure up-regulates the expression of CgRAR and CgRXR. Besides, CgRAR can interact with CgRXR to form a heterodimer complex. |
format |
Text |
author |
Jin, Kaidi Jin, Qianqian Cai, Zhongqiang Huang, Baoyu Wei, Lei Zhang, Meiwei Guo, Wen Liu, Yaqiong Wang, Xiaotong |
author_facet |
Jin, Kaidi Jin, Qianqian Cai, Zhongqiang Huang, Baoyu Wei, Lei Zhang, Meiwei Guo, Wen Liu, Yaqiong Wang, Xiaotong |
author_sort |
Jin, Kaidi |
title |
Molecular Characterization of Retinoic Acid Receptor CgRAR in Pacific Oyster (Crassostrea gigas) |
title_short |
Molecular Characterization of Retinoic Acid Receptor CgRAR in Pacific Oyster (Crassostrea gigas) |
title_full |
Molecular Characterization of Retinoic Acid Receptor CgRAR in Pacific Oyster (Crassostrea gigas) |
title_fullStr |
Molecular Characterization of Retinoic Acid Receptor CgRAR in Pacific Oyster (Crassostrea gigas) |
title_full_unstemmed |
Molecular Characterization of Retinoic Acid Receptor CgRAR in Pacific Oyster (Crassostrea gigas) |
title_sort |
molecular characterization of retinoic acid receptor cgrar in pacific oyster (crassostrea gigas) |
publisher |
Frontiers Media S.A. |
publishDate |
2021 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8060629/ https://doi.org/10.3389/fphys.2021.666842 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Front Physiol |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8060629/ http://dx.doi.org/10.3389/fphys.2021.666842 |
op_rights |
Copyright © 2021 Jin, Jin, Cai, Huang, Wei, Zhang, Guo, Liu and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fphys.2021.666842 |
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Frontiers in Physiology |
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12 |
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1766393673666265088 |