Characterizing the culturable surface microbiomes of diverse marine animals
Biofilm-forming bacteria have the potential to contribute to the health, physiology, behavior and ecology of the host and serve as its first line of defense against adverse conditions in the environment. While metabarcoding and metagenomic information furthers our understanding of microbiome composi...
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012112/ http://www.ncbi.nlm.nih.gov/pubmed/33681975 https://doi.org/10.1093/femsec/fiab040 |
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ftpubmed:oai:pubmedcentral.nih.gov:8012112 2023-05-15T17:10:50+02:00 Characterizing the culturable surface microbiomes of diverse marine animals Keller, Abigail G Apprill, Amy Lebaron, Philippe Robbins, Jooke Romano, Tracy A Overton, Ellysia Rong, Yuying Yuan, Ruiyi Pollara, Scott Whalen, Kristen E 2021-03-03 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012112/ http://www.ncbi.nlm.nih.gov/pubmed/33681975 https://doi.org/10.1093/femsec/fiab040 en eng Oxford University Press http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012112/ http://www.ncbi.nlm.nih.gov/pubmed/33681975 http://dx.doi.org/10.1093/femsec/fiab040 © The Author(s) 2021. Published by Oxford University Press on behalf of FEMS. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com. CC-BY-NC-ND FEMS Microbiol Ecol Research Article Text 2021 ftpubmed https://doi.org/10.1093/femsec/fiab040 2021-04-11T00:26:37Z Biofilm-forming bacteria have the potential to contribute to the health, physiology, behavior and ecology of the host and serve as its first line of defense against adverse conditions in the environment. While metabarcoding and metagenomic information furthers our understanding of microbiome composition, fewer studies use cultured samples to study the diverse interactions among the host and its microbiome, as cultured representatives are often lacking. This study examines the surface microbiomes cultured from three shallow-water coral species and two whale species. These unique marine animals place strong selective pressures on their microbial symbionts and contain members under similar environmental and anthropogenic stress. We developed an intense cultivation procedure, utilizing a suite of culture conditions targeting a rich assortment of biofilm-forming microorganisms. We identified 592 microbial isolates contained within 15 bacterial orders representing 50 bacterial genera, and two fungal species. Culturable bacteria from coral and whale samples paralleled taxonomic groups identified in culture-independent surveys, including 29% of all bacterial genera identified in the Megaptera novaeangliae skin microbiome through culture-independent methods. This microbial repository provides raw material and biological input for more nuanced studies which can explore how members of the microbiome both shape their micro-niche and impact host fitness. Text Megaptera novaeangliae PubMed Central (PMC) FEMS Microbiology Ecology 97 4 |
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English |
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Research Article |
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Research Article Keller, Abigail G Apprill, Amy Lebaron, Philippe Robbins, Jooke Romano, Tracy A Overton, Ellysia Rong, Yuying Yuan, Ruiyi Pollara, Scott Whalen, Kristen E Characterizing the culturable surface microbiomes of diverse marine animals |
topic_facet |
Research Article |
description |
Biofilm-forming bacteria have the potential to contribute to the health, physiology, behavior and ecology of the host and serve as its first line of defense against adverse conditions in the environment. While metabarcoding and metagenomic information furthers our understanding of microbiome composition, fewer studies use cultured samples to study the diverse interactions among the host and its microbiome, as cultured representatives are often lacking. This study examines the surface microbiomes cultured from three shallow-water coral species and two whale species. These unique marine animals place strong selective pressures on their microbial symbionts and contain members under similar environmental and anthropogenic stress. We developed an intense cultivation procedure, utilizing a suite of culture conditions targeting a rich assortment of biofilm-forming microorganisms. We identified 592 microbial isolates contained within 15 bacterial orders representing 50 bacterial genera, and two fungal species. Culturable bacteria from coral and whale samples paralleled taxonomic groups identified in culture-independent surveys, including 29% of all bacterial genera identified in the Megaptera novaeangliae skin microbiome through culture-independent methods. This microbial repository provides raw material and biological input for more nuanced studies which can explore how members of the microbiome both shape their micro-niche and impact host fitness. |
format |
Text |
author |
Keller, Abigail G Apprill, Amy Lebaron, Philippe Robbins, Jooke Romano, Tracy A Overton, Ellysia Rong, Yuying Yuan, Ruiyi Pollara, Scott Whalen, Kristen E |
author_facet |
Keller, Abigail G Apprill, Amy Lebaron, Philippe Robbins, Jooke Romano, Tracy A Overton, Ellysia Rong, Yuying Yuan, Ruiyi Pollara, Scott Whalen, Kristen E |
author_sort |
Keller, Abigail G |
title |
Characterizing the culturable surface microbiomes of diverse marine animals |
title_short |
Characterizing the culturable surface microbiomes of diverse marine animals |
title_full |
Characterizing the culturable surface microbiomes of diverse marine animals |
title_fullStr |
Characterizing the culturable surface microbiomes of diverse marine animals |
title_full_unstemmed |
Characterizing the culturable surface microbiomes of diverse marine animals |
title_sort |
characterizing the culturable surface microbiomes of diverse marine animals |
publisher |
Oxford University Press |
publishDate |
2021 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012112/ http://www.ncbi.nlm.nih.gov/pubmed/33681975 https://doi.org/10.1093/femsec/fiab040 |
genre |
Megaptera novaeangliae |
genre_facet |
Megaptera novaeangliae |
op_source |
FEMS Microbiol Ecol |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012112/ http://www.ncbi.nlm.nih.gov/pubmed/33681975 http://dx.doi.org/10.1093/femsec/fiab040 |
op_rights |
© The Author(s) 2021. Published by Oxford University Press on behalf of FEMS. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com. |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1093/femsec/fiab040 |
container_title |
FEMS Microbiology Ecology |
container_volume |
97 |
container_issue |
4 |
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1766067496687763456 |