Investigating the microbial ecology of coastal hotspots of marine nitrogen fixation in the western North Atlantic
Variation in the microbial cycling of nutrients and carbon in the ocean is an emergent property of complex planktonic communities. While recent findings have considerably expanded our understanding of the diversity and distribution of nitrogen (N(2)) fixing marine diazotrophs, knowledge gaps remain...
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ftpubmed:oai:pubmedcentral.nih.gov:7943828 2023-05-15T17:30:21+02:00 Investigating the microbial ecology of coastal hotspots of marine nitrogen fixation in the western North Atlantic Wang, Seaver Tang, Weiyi Delage, Erwan Gifford, Scott Whitby, Hannah González, Aridane G. Eveillard, Damien Planquette, Hélène Cassar, Nicolas 2021-03-09 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7943828/ https://doi.org/10.1038/s41598-021-84969-1 en eng Nature Publishing Group UK http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7943828/ http://dx.doi.org/10.1038/s41598-021-84969-1 © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. CC-BY Sci Rep Article Text 2021 ftpubmed https://doi.org/10.1038/s41598-021-84969-1 2021-03-14T02:03:43Z Variation in the microbial cycling of nutrients and carbon in the ocean is an emergent property of complex planktonic communities. While recent findings have considerably expanded our understanding of the diversity and distribution of nitrogen (N(2)) fixing marine diazotrophs, knowledge gaps remain regarding ecological interactions between diazotrophs and other community members. Using quantitative 16S and 18S V4 rDNA amplicon sequencing, we surveyed eukaryotic and prokaryotic microbial communities from samples collected in August 2016 and 2017 across the Western North Atlantic. Leveraging and significantly expanding an earlier published 2015 molecular dataset, we examined microbial community structure and ecological co-occurrence relationships associated with intense hotspots of N(2) fixation previously reported at sites off the Southern New England Shelf and Mid-Atlantic Bight. Overall, we observed a negative relationship between eukaryotic diversity and both N(2) fixation and net community production (NCP). Maximum N(2) fixation rates occurred at sites with high abundances of mixotrophic stramenopiles, notably Chrysophyceae. Network analysis revealed such stramenopiles to be keystone taxa alongside the haptophyte diazotroph host Braarudosphaera bigelowii and chlorophytes. Our findings highlight an intriguing relationship between marine stramenopiles and high N(2) fixation coastal sites. Text North Atlantic PubMed Central (PMC) Scientific Reports 11 1 |
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Article Wang, Seaver Tang, Weiyi Delage, Erwan Gifford, Scott Whitby, Hannah González, Aridane G. Eveillard, Damien Planquette, Hélène Cassar, Nicolas Investigating the microbial ecology of coastal hotspots of marine nitrogen fixation in the western North Atlantic |
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Article |
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Variation in the microbial cycling of nutrients and carbon in the ocean is an emergent property of complex planktonic communities. While recent findings have considerably expanded our understanding of the diversity and distribution of nitrogen (N(2)) fixing marine diazotrophs, knowledge gaps remain regarding ecological interactions between diazotrophs and other community members. Using quantitative 16S and 18S V4 rDNA amplicon sequencing, we surveyed eukaryotic and prokaryotic microbial communities from samples collected in August 2016 and 2017 across the Western North Atlantic. Leveraging and significantly expanding an earlier published 2015 molecular dataset, we examined microbial community structure and ecological co-occurrence relationships associated with intense hotspots of N(2) fixation previously reported at sites off the Southern New England Shelf and Mid-Atlantic Bight. Overall, we observed a negative relationship between eukaryotic diversity and both N(2) fixation and net community production (NCP). Maximum N(2) fixation rates occurred at sites with high abundances of mixotrophic stramenopiles, notably Chrysophyceae. Network analysis revealed such stramenopiles to be keystone taxa alongside the haptophyte diazotroph host Braarudosphaera bigelowii and chlorophytes. Our findings highlight an intriguing relationship between marine stramenopiles and high N(2) fixation coastal sites. |
format |
Text |
author |
Wang, Seaver Tang, Weiyi Delage, Erwan Gifford, Scott Whitby, Hannah González, Aridane G. Eveillard, Damien Planquette, Hélène Cassar, Nicolas |
author_facet |
Wang, Seaver Tang, Weiyi Delage, Erwan Gifford, Scott Whitby, Hannah González, Aridane G. Eveillard, Damien Planquette, Hélène Cassar, Nicolas |
author_sort |
Wang, Seaver |
title |
Investigating the microbial ecology of coastal hotspots of marine nitrogen fixation in the western North Atlantic |
title_short |
Investigating the microbial ecology of coastal hotspots of marine nitrogen fixation in the western North Atlantic |
title_full |
Investigating the microbial ecology of coastal hotspots of marine nitrogen fixation in the western North Atlantic |
title_fullStr |
Investigating the microbial ecology of coastal hotspots of marine nitrogen fixation in the western North Atlantic |
title_full_unstemmed |
Investigating the microbial ecology of coastal hotspots of marine nitrogen fixation in the western North Atlantic |
title_sort |
investigating the microbial ecology of coastal hotspots of marine nitrogen fixation in the western north atlantic |
publisher |
Nature Publishing Group UK |
publishDate |
2021 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7943828/ https://doi.org/10.1038/s41598-021-84969-1 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
Sci Rep |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7943828/ http://dx.doi.org/10.1038/s41598-021-84969-1 |
op_rights |
© The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
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CC-BY |
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https://doi.org/10.1038/s41598-021-84969-1 |
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Scientific Reports |
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11 |
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