Protein-Based Vaccine Protect Against Piscirickettsia salmonis in Atlantic Salmon (Salmo salar)
An effective and economical vaccine against the Piscirickettsia salmonis pathogen is needed for sustainable salmon farming and to reduce disease-related economic losses. Consequently, the aquaculture industry urgently needs to investigate efficient prophylactic measures. Three protein-based vaccine...
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ftpubmed:oai:pubmedcentral.nih.gov:7927424 2023-05-15T15:30:51+02:00 Protein-Based Vaccine Protect Against Piscirickettsia salmonis in Atlantic Salmon (Salmo salar) Pontigo, Juan Pablo Espinoza, Carla Hernandez, Mauricio Nourdin, Guillermo Oliver, Cristian Avendaño-Herrera, Rubén Figueroa, Jaime Rauch, Cecilia Troncoso, José M. Vargas-Chacoff, Luis Yáñez, Alejandro J. 2021-02-17 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7927424/ https://doi.org/10.3389/fimmu.2021.602689 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7927424/ http://dx.doi.org/10.3389/fimmu.2021.602689 Copyright © 2021 Pontigo, Espinoza, Hernandez, Nourdin, Oliver, Avendaño-Herrera, Figueroa, Rauch, Troncoso, Vargas-Chacoff and Yáñez. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. CC-BY Front Immunol Immunology Text 2021 ftpubmed https://doi.org/10.3389/fimmu.2021.602689 2021-03-07T02:35:07Z An effective and economical vaccine against the Piscirickettsia salmonis pathogen is needed for sustainable salmon farming and to reduce disease-related economic losses. Consequently, the aquaculture industry urgently needs to investigate efficient prophylactic measures. Three protein-based vaccine prototypes against Piscirickettsia salmonis were prepared from a highly pathogenic Chilean isolate. Only one vaccine effectively protected Atlantic salmon (Salmo salar), in correlation with the induction of Piscirickettsia-specific IgM antibodies and a high induction of transcripts encoding pro-inflammatory cytokines (i.e., Il-1β and TNF-α). In addition, we studied the proteome fraction protein of P. salmonis strain Austral-005 using multidimensional protein identification technology. The analyzes identified 87 proteins of different subcellular origins, such as the cytoplasmic and membrane compartment, where many of them have virulence functions. The other two prototypes activated only the innate immune responses, but did not protect Salmo salar against P. salmonis. These results suggest that the knowledge of the formulation of vaccines based on P. salmonis proteins is useful as an effective therapy, this demonstrates the importance of the different research tools to improve the study of the different immune responses, resistance to diseases in the Atlantic salmon. We suggest that this vaccine can help prevent widespread infection by P. salmonis, in addition to being able to be used as a booster after a primary vaccine to maintain high levels of circulating protective antibodies, greatly helping to reduce the economic losses caused by the pathogen. Text Atlantic salmon Salmo salar PubMed Central (PMC) Austral Frontiers in Immunology 12 |
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PubMed Central (PMC) |
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English |
topic |
Immunology |
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Immunology Pontigo, Juan Pablo Espinoza, Carla Hernandez, Mauricio Nourdin, Guillermo Oliver, Cristian Avendaño-Herrera, Rubén Figueroa, Jaime Rauch, Cecilia Troncoso, José M. Vargas-Chacoff, Luis Yáñez, Alejandro J. Protein-Based Vaccine Protect Against Piscirickettsia salmonis in Atlantic Salmon (Salmo salar) |
topic_facet |
Immunology |
description |
An effective and economical vaccine against the Piscirickettsia salmonis pathogen is needed for sustainable salmon farming and to reduce disease-related economic losses. Consequently, the aquaculture industry urgently needs to investigate efficient prophylactic measures. Three protein-based vaccine prototypes against Piscirickettsia salmonis were prepared from a highly pathogenic Chilean isolate. Only one vaccine effectively protected Atlantic salmon (Salmo salar), in correlation with the induction of Piscirickettsia-specific IgM antibodies and a high induction of transcripts encoding pro-inflammatory cytokines (i.e., Il-1β and TNF-α). In addition, we studied the proteome fraction protein of P. salmonis strain Austral-005 using multidimensional protein identification technology. The analyzes identified 87 proteins of different subcellular origins, such as the cytoplasmic and membrane compartment, where many of them have virulence functions. The other two prototypes activated only the innate immune responses, but did not protect Salmo salar against P. salmonis. These results suggest that the knowledge of the formulation of vaccines based on P. salmonis proteins is useful as an effective therapy, this demonstrates the importance of the different research tools to improve the study of the different immune responses, resistance to diseases in the Atlantic salmon. We suggest that this vaccine can help prevent widespread infection by P. salmonis, in addition to being able to be used as a booster after a primary vaccine to maintain high levels of circulating protective antibodies, greatly helping to reduce the economic losses caused by the pathogen. |
format |
Text |
author |
Pontigo, Juan Pablo Espinoza, Carla Hernandez, Mauricio Nourdin, Guillermo Oliver, Cristian Avendaño-Herrera, Rubén Figueroa, Jaime Rauch, Cecilia Troncoso, José M. Vargas-Chacoff, Luis Yáñez, Alejandro J. |
author_facet |
Pontigo, Juan Pablo Espinoza, Carla Hernandez, Mauricio Nourdin, Guillermo Oliver, Cristian Avendaño-Herrera, Rubén Figueroa, Jaime Rauch, Cecilia Troncoso, José M. Vargas-Chacoff, Luis Yáñez, Alejandro J. |
author_sort |
Pontigo, Juan Pablo |
title |
Protein-Based Vaccine Protect Against Piscirickettsia salmonis in Atlantic Salmon (Salmo salar) |
title_short |
Protein-Based Vaccine Protect Against Piscirickettsia salmonis in Atlantic Salmon (Salmo salar) |
title_full |
Protein-Based Vaccine Protect Against Piscirickettsia salmonis in Atlantic Salmon (Salmo salar) |
title_fullStr |
Protein-Based Vaccine Protect Against Piscirickettsia salmonis in Atlantic Salmon (Salmo salar) |
title_full_unstemmed |
Protein-Based Vaccine Protect Against Piscirickettsia salmonis in Atlantic Salmon (Salmo salar) |
title_sort |
protein-based vaccine protect against piscirickettsia salmonis in atlantic salmon (salmo salar) |
publisher |
Frontiers Media S.A. |
publishDate |
2021 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7927424/ https://doi.org/10.3389/fimmu.2021.602689 |
geographic |
Austral |
geographic_facet |
Austral |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Front Immunol |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7927424/ http://dx.doi.org/10.3389/fimmu.2021.602689 |
op_rights |
Copyright © 2021 Pontigo, Espinoza, Hernandez, Nourdin, Oliver, Avendaño-Herrera, Figueroa, Rauch, Troncoso, Vargas-Chacoff and Yáñez. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fimmu.2021.602689 |
container_title |
Frontiers in Immunology |
container_volume |
12 |
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1766361312659505152 |