Cytogenomics Unveil Possible Transposable Elements Driving Rearrangements in Chromosomes 2 and 4 of Solea senegalensis
Cytogenomics, the integration of cytogenetic and genomic data, has been used here to reconstruct the evolution of chromosomes 2 and 4 of Solea senegalensis. S. senegalensis is a flat fish with a karyotype comprising 2n = 42 chromosomes: 6 metacentric + 4 submetacentric + 8 subtelocentric + 24 teloce...
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ftpubmed:oai:pubmedcentral.nih.gov:7915175 2023-05-15T18:15:51+02:00 Cytogenomics Unveil Possible Transposable Elements Driving Rearrangements in Chromosomes 2 and 4 of Solea senegalensis Rodríguez, María Esther Cross, Ismael Arias-Pérez, Alberto Portela-Bens, Silvia Merlo, Manuel Alejandro Liehr, Thomas Rebordinos, Laureana 2021-02-05 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7915175/ http://www.ncbi.nlm.nih.gov/pubmed/33562667 https://doi.org/10.3390/ijms22041614 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7915175/ http://www.ncbi.nlm.nih.gov/pubmed/33562667 http://dx.doi.org/10.3390/ijms22041614 © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Int J Mol Sci Article Text 2021 ftpubmed https://doi.org/10.3390/ijms22041614 2021-03-07T02:09:14Z Cytogenomics, the integration of cytogenetic and genomic data, has been used here to reconstruct the evolution of chromosomes 2 and 4 of Solea senegalensis. S. senegalensis is a flat fish with a karyotype comprising 2n = 42 chromosomes: 6 metacentric + 4 submetacentric + 8 subtelocentric + 24 telocentric. The Fluorescence in situ Hybridization with Bacterial Artificial Chromosomes (FISH-BAC) technique was applied to locate BACs in these chromosomes (11 and 10 BACs in chromosomes 2 and 4, respectively) and to generate integrated maps. Synteny analysis, taking eight reference fish species (Cynoglossus semilaevis, Scophthalmus maximus, Sparus aurata, Gasterosteus aculeatus, Xiphophorus maculatus, Oryzias latipes, Danio rerio, and Lepisosteus oculatus) for comparison, showed that the BACs of these two chromosomes of S. senegalensis were mainly distributed in two principal chromosomes in the reference species. Transposable Elements (TE) analysis showed significant differences between the two chromosomes, in terms of number of loci per Mb and coverage, and the class of TE (I or II) present. Analysis of TE divergence in chromosomes 2 and 4 compared to their syntenic regions in four reference fish species (C. semilaevis, S. maximus, O. latipes, and D. rerio) revealed differences in their age of activity compared with those species but less notable differences between the two chromosomes. Differences were also observed in peaks of divergence and coverage of TE families for all reference species even in those close to S. senegalensis, like S. maximus and C. semilaevis. Considered together, chromosomes 2 and 4 have evolved by Robertsonian fusions, pericentric inversions, and other chromosomal rearrangements mediated by TEs. Text Scophthalmus maximus PubMed Central (PMC) International Journal of Molecular Sciences 22 4 1614 |
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Article Rodríguez, María Esther Cross, Ismael Arias-Pérez, Alberto Portela-Bens, Silvia Merlo, Manuel Alejandro Liehr, Thomas Rebordinos, Laureana Cytogenomics Unveil Possible Transposable Elements Driving Rearrangements in Chromosomes 2 and 4 of Solea senegalensis |
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Cytogenomics, the integration of cytogenetic and genomic data, has been used here to reconstruct the evolution of chromosomes 2 and 4 of Solea senegalensis. S. senegalensis is a flat fish with a karyotype comprising 2n = 42 chromosomes: 6 metacentric + 4 submetacentric + 8 subtelocentric + 24 telocentric. The Fluorescence in situ Hybridization with Bacterial Artificial Chromosomes (FISH-BAC) technique was applied to locate BACs in these chromosomes (11 and 10 BACs in chromosomes 2 and 4, respectively) and to generate integrated maps. Synteny analysis, taking eight reference fish species (Cynoglossus semilaevis, Scophthalmus maximus, Sparus aurata, Gasterosteus aculeatus, Xiphophorus maculatus, Oryzias latipes, Danio rerio, and Lepisosteus oculatus) for comparison, showed that the BACs of these two chromosomes of S. senegalensis were mainly distributed in two principal chromosomes in the reference species. Transposable Elements (TE) analysis showed significant differences between the two chromosomes, in terms of number of loci per Mb and coverage, and the class of TE (I or II) present. Analysis of TE divergence in chromosomes 2 and 4 compared to their syntenic regions in four reference fish species (C. semilaevis, S. maximus, O. latipes, and D. rerio) revealed differences in their age of activity compared with those species but less notable differences between the two chromosomes. Differences were also observed in peaks of divergence and coverage of TE families for all reference species even in those close to S. senegalensis, like S. maximus and C. semilaevis. Considered together, chromosomes 2 and 4 have evolved by Robertsonian fusions, pericentric inversions, and other chromosomal rearrangements mediated by TEs. |
format |
Text |
author |
Rodríguez, María Esther Cross, Ismael Arias-Pérez, Alberto Portela-Bens, Silvia Merlo, Manuel Alejandro Liehr, Thomas Rebordinos, Laureana |
author_facet |
Rodríguez, María Esther Cross, Ismael Arias-Pérez, Alberto Portela-Bens, Silvia Merlo, Manuel Alejandro Liehr, Thomas Rebordinos, Laureana |
author_sort |
Rodríguez, María Esther |
title |
Cytogenomics Unveil Possible Transposable Elements Driving Rearrangements in Chromosomes 2 and 4 of Solea senegalensis |
title_short |
Cytogenomics Unveil Possible Transposable Elements Driving Rearrangements in Chromosomes 2 and 4 of Solea senegalensis |
title_full |
Cytogenomics Unveil Possible Transposable Elements Driving Rearrangements in Chromosomes 2 and 4 of Solea senegalensis |
title_fullStr |
Cytogenomics Unveil Possible Transposable Elements Driving Rearrangements in Chromosomes 2 and 4 of Solea senegalensis |
title_full_unstemmed |
Cytogenomics Unveil Possible Transposable Elements Driving Rearrangements in Chromosomes 2 and 4 of Solea senegalensis |
title_sort |
cytogenomics unveil possible transposable elements driving rearrangements in chromosomes 2 and 4 of solea senegalensis |
publisher |
MDPI |
publishDate |
2021 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7915175/ http://www.ncbi.nlm.nih.gov/pubmed/33562667 https://doi.org/10.3390/ijms22041614 |
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Scophthalmus maximus |
genre_facet |
Scophthalmus maximus |
op_source |
Int J Mol Sci |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7915175/ http://www.ncbi.nlm.nih.gov/pubmed/33562667 http://dx.doi.org/10.3390/ijms22041614 |
op_rights |
© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
op_doi |
https://doi.org/10.3390/ijms22041614 |
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International Journal of Molecular Sciences |
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22 |
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4 |
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1614 |
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1766189083484225536 |