Genomics Insights into Pseudomonas sp. CG01: An Antarctic Cadmium-Resistant Strain Capable of Biosynthesizing CdS Nanoparticles Using Methionine as S-Source
Here, we present the draft genome sequence of Pseudomonas sp. GC01, a cadmium-resistant Antarctic bacterium capable of biosynthesizing CdS fluorescent nanoparticles (quantum dots, QDs) employing a unique mechanism involving the production of methanethiol (MeSH) from methionine (Met). To explore the...
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ftpubmed:oai:pubmedcentral.nih.gov:7912247 2023-05-15T13:48:31+02:00 Genomics Insights into Pseudomonas sp. CG01: An Antarctic Cadmium-Resistant Strain Capable of Biosynthesizing CdS Nanoparticles Using Methionine as S-Source Gallardo-Benavente, Carla Campo-Giraldo, Jessica L. Castro-Severyn, Juan Quiroz, Andrés Pérez-Donoso, José M. 2021-01-27 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7912247/ http://www.ncbi.nlm.nih.gov/pubmed/33514061 https://doi.org/10.3390/genes12020187 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7912247/ http://www.ncbi.nlm.nih.gov/pubmed/33514061 http://dx.doi.org/10.3390/genes12020187 © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Genes (Basel) Article Text 2021 ftpubmed https://doi.org/10.3390/genes12020187 2021-03-07T02:00:37Z Here, we present the draft genome sequence of Pseudomonas sp. GC01, a cadmium-resistant Antarctic bacterium capable of biosynthesizing CdS fluorescent nanoparticles (quantum dots, QDs) employing a unique mechanism involving the production of methanethiol (MeSH) from methionine (Met). To explore the molecular/metabolic components involved in QDs biosynthesis, we conducted a comparative genomic analysis, searching for the genes related to cadmium resistance and sulfur metabolic pathways. The genome of Pseudomonas sp. GC01 has a 4,706,645 bp size with a 58.61% G+C content. Pseudomonas sp. GC01 possesses five genes related to cadmium transport/resistance, with three P-type ATPases (cadA, zntA, and pbrA) involved in Cd-secretion that could contribute to the extracellular biosynthesis of CdS QDs. Furthermore, it exhibits genes involved in sulfate assimilation, cysteine/methionine synthesis, and volatile sulfur compounds catabolic pathways. Regarding MeSH production from Met, Pseudomonas sp. GC01 lacks the genes E4.4.1.11 and megL for MeSH generation. Interestingly, despite the absence of these genes, Pseudomonas sp. GC01 produces high levels of MeSH. This is probably associated with the metC gene that also produces MeSH from Met in bacteria. This work is the first report of the potential genes involved in Cd resistance, sulfur metabolism, and the process of MeSH-dependent CdS QDs bioproduction in Pseudomonas spp. strains. Text Antarc* Antarctic PubMed Central (PMC) Antarctic Genes 12 2 187 |
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Article Gallardo-Benavente, Carla Campo-Giraldo, Jessica L. Castro-Severyn, Juan Quiroz, Andrés Pérez-Donoso, José M. Genomics Insights into Pseudomonas sp. CG01: An Antarctic Cadmium-Resistant Strain Capable of Biosynthesizing CdS Nanoparticles Using Methionine as S-Source |
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Here, we present the draft genome sequence of Pseudomonas sp. GC01, a cadmium-resistant Antarctic bacterium capable of biosynthesizing CdS fluorescent nanoparticles (quantum dots, QDs) employing a unique mechanism involving the production of methanethiol (MeSH) from methionine (Met). To explore the molecular/metabolic components involved in QDs biosynthesis, we conducted a comparative genomic analysis, searching for the genes related to cadmium resistance and sulfur metabolic pathways. The genome of Pseudomonas sp. GC01 has a 4,706,645 bp size with a 58.61% G+C content. Pseudomonas sp. GC01 possesses five genes related to cadmium transport/resistance, with three P-type ATPases (cadA, zntA, and pbrA) involved in Cd-secretion that could contribute to the extracellular biosynthesis of CdS QDs. Furthermore, it exhibits genes involved in sulfate assimilation, cysteine/methionine synthesis, and volatile sulfur compounds catabolic pathways. Regarding MeSH production from Met, Pseudomonas sp. GC01 lacks the genes E4.4.1.11 and megL for MeSH generation. Interestingly, despite the absence of these genes, Pseudomonas sp. GC01 produces high levels of MeSH. This is probably associated with the metC gene that also produces MeSH from Met in bacteria. This work is the first report of the potential genes involved in Cd resistance, sulfur metabolism, and the process of MeSH-dependent CdS QDs bioproduction in Pseudomonas spp. strains. |
format |
Text |
author |
Gallardo-Benavente, Carla Campo-Giraldo, Jessica L. Castro-Severyn, Juan Quiroz, Andrés Pérez-Donoso, José M. |
author_facet |
Gallardo-Benavente, Carla Campo-Giraldo, Jessica L. Castro-Severyn, Juan Quiroz, Andrés Pérez-Donoso, José M. |
author_sort |
Gallardo-Benavente, Carla |
title |
Genomics Insights into Pseudomonas sp. CG01: An Antarctic Cadmium-Resistant Strain Capable of Biosynthesizing CdS Nanoparticles Using Methionine as S-Source |
title_short |
Genomics Insights into Pseudomonas sp. CG01: An Antarctic Cadmium-Resistant Strain Capable of Biosynthesizing CdS Nanoparticles Using Methionine as S-Source |
title_full |
Genomics Insights into Pseudomonas sp. CG01: An Antarctic Cadmium-Resistant Strain Capable of Biosynthesizing CdS Nanoparticles Using Methionine as S-Source |
title_fullStr |
Genomics Insights into Pseudomonas sp. CG01: An Antarctic Cadmium-Resistant Strain Capable of Biosynthesizing CdS Nanoparticles Using Methionine as S-Source |
title_full_unstemmed |
Genomics Insights into Pseudomonas sp. CG01: An Antarctic Cadmium-Resistant Strain Capable of Biosynthesizing CdS Nanoparticles Using Methionine as S-Source |
title_sort |
genomics insights into pseudomonas sp. cg01: an antarctic cadmium-resistant strain capable of biosynthesizing cds nanoparticles using methionine as s-source |
publisher |
MDPI |
publishDate |
2021 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7912247/ http://www.ncbi.nlm.nih.gov/pubmed/33514061 https://doi.org/10.3390/genes12020187 |
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Antarctic |
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Antarctic |
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Antarc* Antarctic |
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Antarc* Antarctic |
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Genes (Basel) |
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7912247/ http://www.ncbi.nlm.nih.gov/pubmed/33514061 http://dx.doi.org/10.3390/genes12020187 |
op_rights |
© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
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https://doi.org/10.3390/genes12020187 |
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Genes |
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