Macrophage migration inhibitory factor may play a protective role in osteoarthritis
BACKGROUND: Osteoarthritis (OA) is the most prevalent form of arthritis and the major cause of disability and overall diminution of quality of life in the elderly population. Currently there is no cure for OA, partly due to the large gaps in our understanding of its underlying molecular and cellular...
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ftpubmed:oai:pubmedcentral.nih.gov:7896408 2023-05-15T17:22:54+02:00 Macrophage migration inhibitory factor may play a protective role in osteoarthritis Liu, Ming Xie, Zikun Sun, Guang Chen, Liujun Qi, Dake Zhang, Hongwei Xiong, Jieying Furey, Andrew Rahman, Proton Lei, Guanghua Zhai, Guangju 2021-02-20 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7896408/ http://www.ncbi.nlm.nih.gov/pubmed/33610191 https://doi.org/10.1186/s13075-021-02442-w en eng BioMed Central http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7896408/ http://www.ncbi.nlm.nih.gov/pubmed/33610191 http://dx.doi.org/10.1186/s13075-021-02442-w © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. CC0 PDM CC-BY Arthritis Res Ther Research Article Text 2021 ftpubmed https://doi.org/10.1186/s13075-021-02442-w 2021-02-28T01:36:42Z BACKGROUND: Osteoarthritis (OA) is the most prevalent form of arthritis and the major cause of disability and overall diminution of quality of life in the elderly population. Currently there is no cure for OA, partly due to the large gaps in our understanding of its underlying molecular and cellular mechanisms. Macrophage migration inhibitory factor (MIF) is a procytokine that mediates pleiotropic inflammatory effects in inflammatory diseases such as rheumatoid arthritis (RA) and ankylosing spondylitis (AS). However, data on the role of MIF in OA is limited with conflicting results. We undertook this study to investigate the role of MIF in OA by examining MIF genotype, mRNA expression, and protein levels in the Newfoundland Osteoarthritis Study. METHODS: One hundred nineteen end-stage knee/hip OA patients, 16 RA patients, and 113 healthy controls were included in the study. Two polymorphisms in the MIF gene, rs755622, and -794 CATT(5-8), were genotyped using polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) and PCR followed by automated capillary electrophoresis, respectively. MIF mRNA levels in articular cartilage and subchondral bone were measured by quantitative polymerase chain reaction. Plasma concentrations of MIF, tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and interleukin-1 beta (IL-1β) were measured by enzyme-linked immunosorbent assay. RESULTS: rs755622 and -794 CATT(5-8) genotypes were not associated with MIF mRNA or protein levels or OA (all p ≥ 0.19). MIF mRNA level in cartilage was lower in OA patients than in controls (p = 0.028) and RA patients (p = 0.004), while the levels in bone were comparable between OA patients and controls (p = 0.165). MIF protein level in plasma was lower in OA patients than in controls (p = 3.01 × 10(−10)), while the levels of TNF-α, IL-6 and IL-1β in plasma were all significantly higher in OA patients than in controls (all p ≤ 0.0007). Multivariable logistic regression showed lower MIF and higher IL-1β protein levels in plasma ... Text Newfoundland PubMed Central (PMC) Arthritis Research & Therapy 23 1 |
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Research Article Liu, Ming Xie, Zikun Sun, Guang Chen, Liujun Qi, Dake Zhang, Hongwei Xiong, Jieying Furey, Andrew Rahman, Proton Lei, Guanghua Zhai, Guangju Macrophage migration inhibitory factor may play a protective role in osteoarthritis |
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Research Article |
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BACKGROUND: Osteoarthritis (OA) is the most prevalent form of arthritis and the major cause of disability and overall diminution of quality of life in the elderly population. Currently there is no cure for OA, partly due to the large gaps in our understanding of its underlying molecular and cellular mechanisms. Macrophage migration inhibitory factor (MIF) is a procytokine that mediates pleiotropic inflammatory effects in inflammatory diseases such as rheumatoid arthritis (RA) and ankylosing spondylitis (AS). However, data on the role of MIF in OA is limited with conflicting results. We undertook this study to investigate the role of MIF in OA by examining MIF genotype, mRNA expression, and protein levels in the Newfoundland Osteoarthritis Study. METHODS: One hundred nineteen end-stage knee/hip OA patients, 16 RA patients, and 113 healthy controls were included in the study. Two polymorphisms in the MIF gene, rs755622, and -794 CATT(5-8), were genotyped using polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) and PCR followed by automated capillary electrophoresis, respectively. MIF mRNA levels in articular cartilage and subchondral bone were measured by quantitative polymerase chain reaction. Plasma concentrations of MIF, tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and interleukin-1 beta (IL-1β) were measured by enzyme-linked immunosorbent assay. RESULTS: rs755622 and -794 CATT(5-8) genotypes were not associated with MIF mRNA or protein levels or OA (all p ≥ 0.19). MIF mRNA level in cartilage was lower in OA patients than in controls (p = 0.028) and RA patients (p = 0.004), while the levels in bone were comparable between OA patients and controls (p = 0.165). MIF protein level in plasma was lower in OA patients than in controls (p = 3.01 × 10(−10)), while the levels of TNF-α, IL-6 and IL-1β in plasma were all significantly higher in OA patients than in controls (all p ≤ 0.0007). Multivariable logistic regression showed lower MIF and higher IL-1β protein levels in plasma ... |
format |
Text |
author |
Liu, Ming Xie, Zikun Sun, Guang Chen, Liujun Qi, Dake Zhang, Hongwei Xiong, Jieying Furey, Andrew Rahman, Proton Lei, Guanghua Zhai, Guangju |
author_facet |
Liu, Ming Xie, Zikun Sun, Guang Chen, Liujun Qi, Dake Zhang, Hongwei Xiong, Jieying Furey, Andrew Rahman, Proton Lei, Guanghua Zhai, Guangju |
author_sort |
Liu, Ming |
title |
Macrophage migration inhibitory factor may play a protective role in osteoarthritis |
title_short |
Macrophage migration inhibitory factor may play a protective role in osteoarthritis |
title_full |
Macrophage migration inhibitory factor may play a protective role in osteoarthritis |
title_fullStr |
Macrophage migration inhibitory factor may play a protective role in osteoarthritis |
title_full_unstemmed |
Macrophage migration inhibitory factor may play a protective role in osteoarthritis |
title_sort |
macrophage migration inhibitory factor may play a protective role in osteoarthritis |
publisher |
BioMed Central |
publishDate |
2021 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7896408/ http://www.ncbi.nlm.nih.gov/pubmed/33610191 https://doi.org/10.1186/s13075-021-02442-w |
genre |
Newfoundland |
genre_facet |
Newfoundland |
op_source |
Arthritis Res Ther |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7896408/ http://www.ncbi.nlm.nih.gov/pubmed/33610191 http://dx.doi.org/10.1186/s13075-021-02442-w |
op_rights |
© The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
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CC0 PDM CC-BY |
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https://doi.org/10.1186/s13075-021-02442-w |
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