Renal Protective Effect of Beluga Lentil Pretreatment for Ischemia-Reperfusion Injury
MATERIALS AND METHODS: Mice were divided into four groups: normal, untreated, low- (2 mg), and high-dose (8 mg) beluga lentil treatment groups. Beluga lentil was orally administered for 2 weeks, followed by bilateral renal ischemia for 20 min and reperfusion for 30 min. Blood samples and kidney tiss...
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ftpubmed:oai:pubmedcentral.nih.gov:7868138 2023-05-15T15:41:41+02:00 Renal Protective Effect of Beluga Lentil Pretreatment for Ischemia-Reperfusion Injury Lee, Syng-ook Chun, So Young Lee, EunHye Kim, Bomi Yoon, BoHyun Gil, Haejung Kim, Dae Hwan Jang, Byung Ik Lee, Dong Woo Yoo, Eun Sang Park, Dong Jin Lee, Jun Nyung Han, Man-Hoon Kim, Bum Soo Song, Phil Hyun Kwon, Tae Gyun Ha, Yun-Sok 2021-01-31 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7868138/ https://doi.org/10.1155/2021/6890679 en eng Hindawi http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7868138/ http://dx.doi.org/10.1155/2021/6890679 Copyright © 2021 Syng-ook Lee et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. CC-BY Biomed Res Int Research Article Text 2021 ftpubmed https://doi.org/10.1155/2021/6890679 2021-02-21T01:25:22Z MATERIALS AND METHODS: Mice were divided into four groups: normal, untreated, low- (2 mg), and high-dose (8 mg) beluga lentil treatment groups. Beluga lentil was orally administered for 2 weeks, followed by bilateral renal ischemia for 20 min and reperfusion for 30 min. Blood samples and kidney tissues were collected and analyzed to investigate renal function, histopathology, epithelial and endothelial cell damage, apoptosis, oxidative stress, and inflammatory responses. RESULTS: The pretreated groups maintained renal function, with significantly lower blood urea nitrogen (BUN) and creatinine levels, compared with the other groups. The histopathological analysis showed reduced proximal tubule injury and decreased injury-related molecule (kidney injury molecule 1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL)) secretion in the pretreated groups compared with the other groups. Terminal deoxynucleotidyl transferase dUTP nick-end labeling- (TUNEL-) positive cells and the secretion of apoptosis-related molecules (Fas and caspase 3) were significantly reduced in the pretreated groups compared with the other groups. The pretreated groups showed positive microvessel-associated gene (cluster of differentiation (CD31)) expression and negative adhesion molecule (intracellular adhesion molecule 1 (ICAM-1)) expression. An antioxidant effect was observed in the pretreatment groups, with reduced malonaldehyde (MDA) expression and increased antioxidant enzyme (superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), and glutathione peroxidase (GPx)) secretion. In the pretreated groups, F4/80+ macrophages and CD4+ T cell infiltration were inhibited and proinflammatory cytokine (interleukin- (IL-) 1β, IL-6, and tumor necrosis factor- (TNF-) α) levels decreased; however, the levels of anti-inflammatory cytokines (transforming growth factor- (TGF-) β, IL-10, and IL-22) increased. CONCLUSIONS: Beluga lentil pretreatment demonstrated protective effects against I/R-induced renal damage, via antiapoptotic, ... Text Beluga Beluga* PubMed Central (PMC) BioMed Research International 2021 1 10 |
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Research Article Lee, Syng-ook Chun, So Young Lee, EunHye Kim, Bomi Yoon, BoHyun Gil, Haejung Kim, Dae Hwan Jang, Byung Ik Lee, Dong Woo Yoo, Eun Sang Park, Dong Jin Lee, Jun Nyung Han, Man-Hoon Kim, Bum Soo Song, Phil Hyun Kwon, Tae Gyun Ha, Yun-Sok Renal Protective Effect of Beluga Lentil Pretreatment for Ischemia-Reperfusion Injury |
topic_facet |
Research Article |
description |
MATERIALS AND METHODS: Mice were divided into four groups: normal, untreated, low- (2 mg), and high-dose (8 mg) beluga lentil treatment groups. Beluga lentil was orally administered for 2 weeks, followed by bilateral renal ischemia for 20 min and reperfusion for 30 min. Blood samples and kidney tissues were collected and analyzed to investigate renal function, histopathology, epithelial and endothelial cell damage, apoptosis, oxidative stress, and inflammatory responses. RESULTS: The pretreated groups maintained renal function, with significantly lower blood urea nitrogen (BUN) and creatinine levels, compared with the other groups. The histopathological analysis showed reduced proximal tubule injury and decreased injury-related molecule (kidney injury molecule 1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL)) secretion in the pretreated groups compared with the other groups. Terminal deoxynucleotidyl transferase dUTP nick-end labeling- (TUNEL-) positive cells and the secretion of apoptosis-related molecules (Fas and caspase 3) were significantly reduced in the pretreated groups compared with the other groups. The pretreated groups showed positive microvessel-associated gene (cluster of differentiation (CD31)) expression and negative adhesion molecule (intracellular adhesion molecule 1 (ICAM-1)) expression. An antioxidant effect was observed in the pretreatment groups, with reduced malonaldehyde (MDA) expression and increased antioxidant enzyme (superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), and glutathione peroxidase (GPx)) secretion. In the pretreated groups, F4/80+ macrophages and CD4+ T cell infiltration were inhibited and proinflammatory cytokine (interleukin- (IL-) 1β, IL-6, and tumor necrosis factor- (TNF-) α) levels decreased; however, the levels of anti-inflammatory cytokines (transforming growth factor- (TGF-) β, IL-10, and IL-22) increased. CONCLUSIONS: Beluga lentil pretreatment demonstrated protective effects against I/R-induced renal damage, via antiapoptotic, ... |
format |
Text |
author |
Lee, Syng-ook Chun, So Young Lee, EunHye Kim, Bomi Yoon, BoHyun Gil, Haejung Kim, Dae Hwan Jang, Byung Ik Lee, Dong Woo Yoo, Eun Sang Park, Dong Jin Lee, Jun Nyung Han, Man-Hoon Kim, Bum Soo Song, Phil Hyun Kwon, Tae Gyun Ha, Yun-Sok |
author_facet |
Lee, Syng-ook Chun, So Young Lee, EunHye Kim, Bomi Yoon, BoHyun Gil, Haejung Kim, Dae Hwan Jang, Byung Ik Lee, Dong Woo Yoo, Eun Sang Park, Dong Jin Lee, Jun Nyung Han, Man-Hoon Kim, Bum Soo Song, Phil Hyun Kwon, Tae Gyun Ha, Yun-Sok |
author_sort |
Lee, Syng-ook |
title |
Renal Protective Effect of Beluga Lentil Pretreatment for Ischemia-Reperfusion Injury |
title_short |
Renal Protective Effect of Beluga Lentil Pretreatment for Ischemia-Reperfusion Injury |
title_full |
Renal Protective Effect of Beluga Lentil Pretreatment for Ischemia-Reperfusion Injury |
title_fullStr |
Renal Protective Effect of Beluga Lentil Pretreatment for Ischemia-Reperfusion Injury |
title_full_unstemmed |
Renal Protective Effect of Beluga Lentil Pretreatment for Ischemia-Reperfusion Injury |
title_sort |
renal protective effect of beluga lentil pretreatment for ischemia-reperfusion injury |
publisher |
Hindawi |
publishDate |
2021 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7868138/ https://doi.org/10.1155/2021/6890679 |
genre |
Beluga Beluga* |
genre_facet |
Beluga Beluga* |
op_source |
Biomed Res Int |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7868138/ http://dx.doi.org/10.1155/2021/6890679 |
op_rights |
Copyright © 2021 Syng-ook Lee et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1155/2021/6890679 |
container_title |
BioMed Research International |
container_volume |
2021 |
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1 |
op_container_end_page |
10 |
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1766374575128444928 |