Stress Impairs Skin Barrier Function and Induces α2-3 Linked N-Acetylneuraminic Acid and Core 1 O-Glycans on Skin Mucins in Atlantic Salmon, Salmo salar
The skin barrier consists of mucus, primarily comprising highly glycosylated mucins, and the epithelium. Host mucin glycosylation governs interactions with pathogens and stress is associated with impaired epithelial barrier function. We characterized Atlantic salmon skin barrier function during chro...
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ftpubmed:oai:pubmedcentral.nih.gov:7867331 2023-05-15T15:31:25+02:00 Stress Impairs Skin Barrier Function and Induces α2-3 Linked N-Acetylneuraminic Acid and Core 1 O-Glycans on Skin Mucins in Atlantic Salmon, Salmo salar Benktander, John Sundh, Henrik Sundell, Kristina Murugan, Abarna V. M. Venkatakrishnan, Vignesh Padra, János Tamás Kolarevic, Jelena Terjesen, Bendik Fyhn Gorissen, Marnix Lindén, Sara K. 2021-02-02 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7867331/ http://www.ncbi.nlm.nih.gov/pubmed/33540792 https://doi.org/10.3390/ijms22031488 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7867331/ http://www.ncbi.nlm.nih.gov/pubmed/33540792 http://dx.doi.org/10.3390/ijms22031488 © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Int J Mol Sci Article Text 2021 ftpubmed https://doi.org/10.3390/ijms22031488 2021-02-14T01:34:27Z The skin barrier consists of mucus, primarily comprising highly glycosylated mucins, and the epithelium. Host mucin glycosylation governs interactions with pathogens and stress is associated with impaired epithelial barrier function. We characterized Atlantic salmon skin barrier function during chronic stress (high density) and mucin O-glycosylation changes in response to acute and chronic stress. Fish held at low (LD: 14–30 kg/m(3)) and high densities (HD: 50-80 kg/m(3)) were subjected to acute stress 24 h before sampling at 17 and 21 weeks after start of the experiment. Blood parameters indicated primary and secondary stress responses at both sampling points. At the second sampling, skin barrier function towards molecules was reduced in the HD compared to the LD group (P(app) mannitol; p < 0.01). Liquid chromatography–mass spectrometry revealed 81 O-glycan structures from the skin. Fish subjected to both chronic and acute stress had an increased proportion of large O-glycan structures. Overall, four of the O-glycan changes have potential as indicators of stress, especially for the combined chronic and acute stress. Stress thus impairs skin barrier function and induces glycosylation changes, which have potential to both affect interactions with pathogens and serve as stress indicators. Text Atlantic salmon Salmo salar PubMed Central (PMC) International Journal of Molecular Sciences 22 3 1488 |
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Article Benktander, John Sundh, Henrik Sundell, Kristina Murugan, Abarna V. M. Venkatakrishnan, Vignesh Padra, János Tamás Kolarevic, Jelena Terjesen, Bendik Fyhn Gorissen, Marnix Lindén, Sara K. Stress Impairs Skin Barrier Function and Induces α2-3 Linked N-Acetylneuraminic Acid and Core 1 O-Glycans on Skin Mucins in Atlantic Salmon, Salmo salar |
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Article |
description |
The skin barrier consists of mucus, primarily comprising highly glycosylated mucins, and the epithelium. Host mucin glycosylation governs interactions with pathogens and stress is associated with impaired epithelial barrier function. We characterized Atlantic salmon skin barrier function during chronic stress (high density) and mucin O-glycosylation changes in response to acute and chronic stress. Fish held at low (LD: 14–30 kg/m(3)) and high densities (HD: 50-80 kg/m(3)) were subjected to acute stress 24 h before sampling at 17 and 21 weeks after start of the experiment. Blood parameters indicated primary and secondary stress responses at both sampling points. At the second sampling, skin barrier function towards molecules was reduced in the HD compared to the LD group (P(app) mannitol; p < 0.01). Liquid chromatography–mass spectrometry revealed 81 O-glycan structures from the skin. Fish subjected to both chronic and acute stress had an increased proportion of large O-glycan structures. Overall, four of the O-glycan changes have potential as indicators of stress, especially for the combined chronic and acute stress. Stress thus impairs skin barrier function and induces glycosylation changes, which have potential to both affect interactions with pathogens and serve as stress indicators. |
format |
Text |
author |
Benktander, John Sundh, Henrik Sundell, Kristina Murugan, Abarna V. M. Venkatakrishnan, Vignesh Padra, János Tamás Kolarevic, Jelena Terjesen, Bendik Fyhn Gorissen, Marnix Lindén, Sara K. |
author_facet |
Benktander, John Sundh, Henrik Sundell, Kristina Murugan, Abarna V. M. Venkatakrishnan, Vignesh Padra, János Tamás Kolarevic, Jelena Terjesen, Bendik Fyhn Gorissen, Marnix Lindén, Sara K. |
author_sort |
Benktander, John |
title |
Stress Impairs Skin Barrier Function and Induces α2-3 Linked N-Acetylneuraminic Acid and Core 1 O-Glycans on Skin Mucins in Atlantic Salmon, Salmo salar |
title_short |
Stress Impairs Skin Barrier Function and Induces α2-3 Linked N-Acetylneuraminic Acid and Core 1 O-Glycans on Skin Mucins in Atlantic Salmon, Salmo salar |
title_full |
Stress Impairs Skin Barrier Function and Induces α2-3 Linked N-Acetylneuraminic Acid and Core 1 O-Glycans on Skin Mucins in Atlantic Salmon, Salmo salar |
title_fullStr |
Stress Impairs Skin Barrier Function and Induces α2-3 Linked N-Acetylneuraminic Acid and Core 1 O-Glycans on Skin Mucins in Atlantic Salmon, Salmo salar |
title_full_unstemmed |
Stress Impairs Skin Barrier Function and Induces α2-3 Linked N-Acetylneuraminic Acid and Core 1 O-Glycans on Skin Mucins in Atlantic Salmon, Salmo salar |
title_sort |
stress impairs skin barrier function and induces α2-3 linked n-acetylneuraminic acid and core 1 o-glycans on skin mucins in atlantic salmon, salmo salar |
publisher |
MDPI |
publishDate |
2021 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7867331/ http://www.ncbi.nlm.nih.gov/pubmed/33540792 https://doi.org/10.3390/ijms22031488 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Int J Mol Sci |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7867331/ http://www.ncbi.nlm.nih.gov/pubmed/33540792 http://dx.doi.org/10.3390/ijms22031488 |
op_rights |
© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
op_doi |
https://doi.org/10.3390/ijms22031488 |
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International Journal of Molecular Sciences |
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22 |
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3 |
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1488 |
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1766361910923493376 |