Molecular Decoration of Ceramic Supports for Highly Effective Enzyme Immobilization—Material Approach

A highly effective method was developed to functionalize ceramic supports (Al(2)O(3) powders and membranes) using newly synthesized spacer molecules. The functionalized materials were subsequently utilized for Candida antarctica lipase B enzyme immobilization. The objective is to systematically eval...

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Bibliographic Details
Published in:Materials
Main Authors: Kujawa, Joanna, Głodek, Marta, Koter, Izabela, Ośmiałowski, Borys, Knozowska, Katarzyna, Al-Gharabli, Samer, Dumée, Ludovic F., Kujawski, Wojciech
Format: Text
Language:English
Published: MDPI 2021
Subjects:
Article
Antarc*
Antarctica
Online Access:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7794798/
http://www.ncbi.nlm.nih.gov/pubmed/33401646
https://doi.org/10.3390/ma14010201
Description
Summary:A highly effective method was developed to functionalize ceramic supports (Al(2)O(3) powders and membranes) using newly synthesized spacer molecules. The functionalized materials were subsequently utilized for Candida antarctica lipase B enzyme immobilization. The objective is to systematically evaluate the impact of various spacer molecules grafted onto the alumina materials will affect both the immobilization of the enzymes and specific material surface properties, critical to enzymatic reactors performance. The enzyme loading was significantly improved for the supports modified with shorter spacer molecules, which possessed higher grafting effectiveness on the order of 90%. The specific enzyme activity was found to be much higher for samples functionalized with longer modifiers yielding excellent enantioselectivity >97%. However, the enantiomeric ratio of the immobilized lipase was slightly lower in the case of shorter spacer molecules.

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