A Multiplex Immunosensor for Detecting Perchlorate-Reducing Bacteria for Environmental Monitoring and Planetary Exploration
Perchlorate anions are produced by chemical industries and are important contaminants in certain natural ecosystems. Perchlorate also occurs in some natural and uncontaminated environments such as the Atacama Desert, the high Arctic or the Antarctic Dry Valleys, and is especially abundant on the sur...
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ftpubmed:oai:pubmedcentral.nih.gov:7772991 2023-05-15T13:38:05+02:00 A Multiplex Immunosensor for Detecting Perchlorate-Reducing Bacteria for Environmental Monitoring and Planetary Exploration Gallardo-Carreño, Ignacio Moreno-Paz, Mercedes Aguirre, Jacobo Blanco, Yolanda Alonso-Pintado, Eduardo Raymond-Bouchard, Isabelle Maggiori, Catherine Rivas, Luis A. Engelbrektson, Anna Whyte, Lyle Parro, Víctor 2020-12-16 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7772991/ https://doi.org/10.3389/fmicb.2020.590736 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7772991/ http://dx.doi.org/10.3389/fmicb.2020.590736 Copyright © 2020 Gallardo-Carreño, Moreno-Paz, Aguirre, Blanco, Alonso-Pintado, Raymond-Bouchard, Maggiori, Rivas, Engelbrektson, Whyte and Parro. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. CC-BY Front Microbiol Microbiology Text 2020 ftpubmed https://doi.org/10.3389/fmicb.2020.590736 2021-01-03T02:04:31Z Perchlorate anions are produced by chemical industries and are important contaminants in certain natural ecosystems. Perchlorate also occurs in some natural and uncontaminated environments such as the Atacama Desert, the high Arctic or the Antarctic Dry Valleys, and is especially abundant on the surface of Mars. As some bacterial strains are capable of using perchlorate as an electron acceptor under anaerobic conditions, their detection is relevant for environmental monitoring on Earth as well as for the search for life on Mars. We have developed an antibody microarray with 20 polyclonal antibodies to detect perchlorate-reducing bacteria (PRB) strains and two crucial and highly conserved enzymes involved in perchlorate respiration: perchlorate reductase and chlorite dismutase. We determined the cross-reactivity, the working concentration, and the limit of detection of each antibody individually and in a multiplex format by Fluorescent Sandwich Microarray Immunoassay. Although most of them exhibited relatively high sensitivity and specificity, we applied a deconvolution method based on graph theory to discriminate between specific signals and cross-reactions from related microorganisms. We validated the system by analyzing multiple bacterial isolates, crude extracts from contaminated reactors and salt-rich natural samples from the high Arctic. The PRB detecting chip (PRBCHIP) allowed us to detect and classify environmental isolates as well as to detect similar strains by using crude extracts obtained from 0.5 g even from soils with low organic-matter levels (<10(3) cells/g of soil). Our results demonstrated that PRBCHIP is a valuable tool for sensitive and reliable detection of perchlorate-reducing bacteria for research purposes, environmental monitoring and planetary exploration. Text Antarc* Antarctic Arctic PubMed Central (PMC) Antarctic Arctic The Antarctic Frontiers in Microbiology 11 |
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English |
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Microbiology |
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Microbiology Gallardo-Carreño, Ignacio Moreno-Paz, Mercedes Aguirre, Jacobo Blanco, Yolanda Alonso-Pintado, Eduardo Raymond-Bouchard, Isabelle Maggiori, Catherine Rivas, Luis A. Engelbrektson, Anna Whyte, Lyle Parro, Víctor A Multiplex Immunosensor for Detecting Perchlorate-Reducing Bacteria for Environmental Monitoring and Planetary Exploration |
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Microbiology |
description |
Perchlorate anions are produced by chemical industries and are important contaminants in certain natural ecosystems. Perchlorate also occurs in some natural and uncontaminated environments such as the Atacama Desert, the high Arctic or the Antarctic Dry Valleys, and is especially abundant on the surface of Mars. As some bacterial strains are capable of using perchlorate as an electron acceptor under anaerobic conditions, their detection is relevant for environmental monitoring on Earth as well as for the search for life on Mars. We have developed an antibody microarray with 20 polyclonal antibodies to detect perchlorate-reducing bacteria (PRB) strains and two crucial and highly conserved enzymes involved in perchlorate respiration: perchlorate reductase and chlorite dismutase. We determined the cross-reactivity, the working concentration, and the limit of detection of each antibody individually and in a multiplex format by Fluorescent Sandwich Microarray Immunoassay. Although most of them exhibited relatively high sensitivity and specificity, we applied a deconvolution method based on graph theory to discriminate between specific signals and cross-reactions from related microorganisms. We validated the system by analyzing multiple bacterial isolates, crude extracts from contaminated reactors and salt-rich natural samples from the high Arctic. The PRB detecting chip (PRBCHIP) allowed us to detect and classify environmental isolates as well as to detect similar strains by using crude extracts obtained from 0.5 g even from soils with low organic-matter levels (<10(3) cells/g of soil). Our results demonstrated that PRBCHIP is a valuable tool for sensitive and reliable detection of perchlorate-reducing bacteria for research purposes, environmental monitoring and planetary exploration. |
format |
Text |
author |
Gallardo-Carreño, Ignacio Moreno-Paz, Mercedes Aguirre, Jacobo Blanco, Yolanda Alonso-Pintado, Eduardo Raymond-Bouchard, Isabelle Maggiori, Catherine Rivas, Luis A. Engelbrektson, Anna Whyte, Lyle Parro, Víctor |
author_facet |
Gallardo-Carreño, Ignacio Moreno-Paz, Mercedes Aguirre, Jacobo Blanco, Yolanda Alonso-Pintado, Eduardo Raymond-Bouchard, Isabelle Maggiori, Catherine Rivas, Luis A. Engelbrektson, Anna Whyte, Lyle Parro, Víctor |
author_sort |
Gallardo-Carreño, Ignacio |
title |
A Multiplex Immunosensor for Detecting Perchlorate-Reducing Bacteria for Environmental Monitoring and Planetary Exploration |
title_short |
A Multiplex Immunosensor for Detecting Perchlorate-Reducing Bacteria for Environmental Monitoring and Planetary Exploration |
title_full |
A Multiplex Immunosensor for Detecting Perchlorate-Reducing Bacteria for Environmental Monitoring and Planetary Exploration |
title_fullStr |
A Multiplex Immunosensor for Detecting Perchlorate-Reducing Bacteria for Environmental Monitoring and Planetary Exploration |
title_full_unstemmed |
A Multiplex Immunosensor for Detecting Perchlorate-Reducing Bacteria for Environmental Monitoring and Planetary Exploration |
title_sort |
multiplex immunosensor for detecting perchlorate-reducing bacteria for environmental monitoring and planetary exploration |
publisher |
Frontiers Media S.A. |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7772991/ https://doi.org/10.3389/fmicb.2020.590736 |
geographic |
Antarctic Arctic The Antarctic |
geographic_facet |
Antarctic Arctic The Antarctic |
genre |
Antarc* Antarctic Arctic |
genre_facet |
Antarc* Antarctic Arctic |
op_source |
Front Microbiol |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7772991/ http://dx.doi.org/10.3389/fmicb.2020.590736 |
op_rights |
Copyright © 2020 Gallardo-Carreño, Moreno-Paz, Aguirre, Blanco, Alonso-Pintado, Raymond-Bouchard, Maggiori, Rivas, Engelbrektson, Whyte and Parro. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2020.590736 |
container_title |
Frontiers in Microbiology |
container_volume |
11 |
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1766101369337413632 |