Development of a Piscirickettsia salmonis immersion challenge model to investigate the comparative susceptibility of three salmon species
Piscirickettsia salmonis, the aetiological agent of salmonid rickettsial septicaemia (SRS), is a global pathogen of wild and cultured marine salmonids. Here, we describe the development and application of a reproducible, standardized immersion challenge model to induce clinical SRS in juvenile pink...
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ftpubmed:oai:pubmedcentral.nih.gov:7756497 2023-05-15T15:30:36+02:00 Development of a Piscirickettsia salmonis immersion challenge model to investigate the comparative susceptibility of three salmon species Long, Amy Goodall, Aidan Jones, Simon R.M. 2020-10-16 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7756497/ http://www.ncbi.nlm.nih.gov/pubmed/33067883 https://doi.org/10.1111/jfd.13261 en eng John Wiley and Sons Inc. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7756497/ http://www.ncbi.nlm.nih.gov/pubmed/33067883 http://dx.doi.org/10.1111/jfd.13261 © 2020 Her Majesty the Queen in Right of Canada. Journal of Fish Diseases published by John Wiley & Sons Ltd. Reproduced with the permission of the Minister of Fisheries and Oceans Canada. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. CC-BY J Fish Dis Original Articles Text 2020 ftpubmed https://doi.org/10.1111/jfd.13261 2021-01-03T01:39:28Z Piscirickettsia salmonis, the aetiological agent of salmonid rickettsial septicaemia (SRS), is a global pathogen of wild and cultured marine salmonids. Here, we describe the development and application of a reproducible, standardized immersion challenge model to induce clinical SRS in juvenile pink (Oncorhynchus gorbuscha), Atlantic (Salmo salar) and sockeye salmon (O. nerka). Following a 1‐hr immersion in 10(5) colony‐forming units/ml, cumulative mortality in Atlantic salmon was 63.2% while mortality in sockeye salmon was 10%. Prevalence and levels of the bacterium in kidney prior to onset of mortality were lower in sockeye compared with Atlantic or pink salmon. The timing and magnitude of bacterial shedding were estimated from water samples collected during the exposure trials. Shedding was estimated to be 82‐fold higher in Atlantic salmon as compared to sockeye salmon and peaked in the Atlantic salmon trial at 36 d post‐immersion. These data suggest sockeye salmon are less susceptible to P. salmonis than Atlantic or pink salmon. Finally, skin lesions were observed on infected fish during all trials, often in the absence of detectable infection in kidney. As a result, we hypothesize that skin is the primary point of entry for P. salmonis during the immersion challenge. Text Atlantic salmon Oncorhynchus gorbuscha Pink salmon Salmo salar PubMed Central (PMC) Sockeye ENVELOPE(-130.143,-130.143,54.160,54.160) Journal of Fish Diseases 44 1 1 9 |
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English |
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Original Articles |
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Original Articles Long, Amy Goodall, Aidan Jones, Simon R.M. Development of a Piscirickettsia salmonis immersion challenge model to investigate the comparative susceptibility of three salmon species |
topic_facet |
Original Articles |
description |
Piscirickettsia salmonis, the aetiological agent of salmonid rickettsial septicaemia (SRS), is a global pathogen of wild and cultured marine salmonids. Here, we describe the development and application of a reproducible, standardized immersion challenge model to induce clinical SRS in juvenile pink (Oncorhynchus gorbuscha), Atlantic (Salmo salar) and sockeye salmon (O. nerka). Following a 1‐hr immersion in 10(5) colony‐forming units/ml, cumulative mortality in Atlantic salmon was 63.2% while mortality in sockeye salmon was 10%. Prevalence and levels of the bacterium in kidney prior to onset of mortality were lower in sockeye compared with Atlantic or pink salmon. The timing and magnitude of bacterial shedding were estimated from water samples collected during the exposure trials. Shedding was estimated to be 82‐fold higher in Atlantic salmon as compared to sockeye salmon and peaked in the Atlantic salmon trial at 36 d post‐immersion. These data suggest sockeye salmon are less susceptible to P. salmonis than Atlantic or pink salmon. Finally, skin lesions were observed on infected fish during all trials, often in the absence of detectable infection in kidney. As a result, we hypothesize that skin is the primary point of entry for P. salmonis during the immersion challenge. |
format |
Text |
author |
Long, Amy Goodall, Aidan Jones, Simon R.M. |
author_facet |
Long, Amy Goodall, Aidan Jones, Simon R.M. |
author_sort |
Long, Amy |
title |
Development of a Piscirickettsia salmonis immersion challenge model to investigate the comparative susceptibility of three salmon species |
title_short |
Development of a Piscirickettsia salmonis immersion challenge model to investigate the comparative susceptibility of three salmon species |
title_full |
Development of a Piscirickettsia salmonis immersion challenge model to investigate the comparative susceptibility of three salmon species |
title_fullStr |
Development of a Piscirickettsia salmonis immersion challenge model to investigate the comparative susceptibility of three salmon species |
title_full_unstemmed |
Development of a Piscirickettsia salmonis immersion challenge model to investigate the comparative susceptibility of three salmon species |
title_sort |
development of a piscirickettsia salmonis immersion challenge model to investigate the comparative susceptibility of three salmon species |
publisher |
John Wiley and Sons Inc. |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7756497/ http://www.ncbi.nlm.nih.gov/pubmed/33067883 https://doi.org/10.1111/jfd.13261 |
long_lat |
ENVELOPE(-130.143,-130.143,54.160,54.160) |
geographic |
Sockeye |
geographic_facet |
Sockeye |
genre |
Atlantic salmon Oncorhynchus gorbuscha Pink salmon Salmo salar |
genre_facet |
Atlantic salmon Oncorhynchus gorbuscha Pink salmon Salmo salar |
op_source |
J Fish Dis |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7756497/ http://www.ncbi.nlm.nih.gov/pubmed/33067883 http://dx.doi.org/10.1111/jfd.13261 |
op_rights |
© 2020 Her Majesty the Queen in Right of Canada. Journal of Fish Diseases published by John Wiley & Sons Ltd. Reproduced with the permission of the Minister of Fisheries and Oceans Canada. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1111/jfd.13261 |
container_title |
Journal of Fish Diseases |
container_volume |
44 |
container_issue |
1 |
container_start_page |
1 |
op_container_end_page |
9 |
_version_ |
1766361048457150464 |