DNA metabarcoding uncovers fungal diversity in soils of protected and non-protected areas on Deception Island, Antarctica
We assessed soil fungal diversity at two sites on Deception Island, South Shetland Islands, Antarctica using DNA metabarcoding analysis. The first site was a relatively undisturbed area, and the second was much more heavily impacted by research and tourism. We detected 346 fungal amplicon sequence v...
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ftpubmed:oai:pubmedcentral.nih.gov:7738542 2023-05-15T13:45:52+02:00 DNA metabarcoding uncovers fungal diversity in soils of protected and non-protected areas on Deception Island, Antarctica Rosa, Luiz Henrique da Silva, Thamar Holanda Ogaki, Mayara Baptistucci Pinto, Otávio Henrique Bezerra Stech, Michael Convey, Peter Carvalho-Silva, Micheline Rosa, Carlos Augusto Câmara, Paulo E. A. S. 2020-12-15 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738542/ http://www.ncbi.nlm.nih.gov/pubmed/33319803 https://doi.org/10.1038/s41598-020-78934-7 en eng Nature Publishing Group UK http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738542/ http://www.ncbi.nlm.nih.gov/pubmed/33319803 http://dx.doi.org/10.1038/s41598-020-78934-7 © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. CC-BY Sci Rep Article Text 2020 ftpubmed https://doi.org/10.1038/s41598-020-78934-7 2020-12-20T01:44:18Z We assessed soil fungal diversity at two sites on Deception Island, South Shetland Islands, Antarctica using DNA metabarcoding analysis. The first site was a relatively undisturbed area, and the second was much more heavily impacted by research and tourism. We detected 346 fungal amplicon sequence variants dominated by the phyla Ascomycota, Basidiomycota, Mortierellomycota and Chytridiomycota. We also detected taxa belonging to the rare phyla Mucoromycota and Rozellomycota, which have been difficult to detect in Antarctica by traditional isolation methods. Cladosporium sp., Pseudogymnoascus roseus, Leotiomycetes sp. 2, Penicillium sp., Mortierella sp. 1, Mortierella sp. 2, Pseudogymnoascus appendiculatus and Pseudogymnoascus sp. were the most dominant fungi. In addition, 440,153 of the total of 1,214,875 reads detected could be classified only at the level of Fungi. In both sampling areas the DNA of opportunistic, phytopathogenic and symbiotic fungi were detected, which might have been introduced by human activities, transported by birds or wind, and/or represent resident fungi not previously reported from Antarctica. Further long-term studies are required to elucidate how biological colonization in the island may be affected by climatic changes and/or other anthropogenic influences. Text Antarc* Antarctica Deception Island South Shetland Islands PubMed Central (PMC) Deception Island ENVELOPE(-60.633,-60.633,-62.950,-62.950) South Shetland Islands Scientific Reports 10 1 |
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Article Rosa, Luiz Henrique da Silva, Thamar Holanda Ogaki, Mayara Baptistucci Pinto, Otávio Henrique Bezerra Stech, Michael Convey, Peter Carvalho-Silva, Micheline Rosa, Carlos Augusto Câmara, Paulo E. A. S. DNA metabarcoding uncovers fungal diversity in soils of protected and non-protected areas on Deception Island, Antarctica |
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Article |
description |
We assessed soil fungal diversity at two sites on Deception Island, South Shetland Islands, Antarctica using DNA metabarcoding analysis. The first site was a relatively undisturbed area, and the second was much more heavily impacted by research and tourism. We detected 346 fungal amplicon sequence variants dominated by the phyla Ascomycota, Basidiomycota, Mortierellomycota and Chytridiomycota. We also detected taxa belonging to the rare phyla Mucoromycota and Rozellomycota, which have been difficult to detect in Antarctica by traditional isolation methods. Cladosporium sp., Pseudogymnoascus roseus, Leotiomycetes sp. 2, Penicillium sp., Mortierella sp. 1, Mortierella sp. 2, Pseudogymnoascus appendiculatus and Pseudogymnoascus sp. were the most dominant fungi. In addition, 440,153 of the total of 1,214,875 reads detected could be classified only at the level of Fungi. In both sampling areas the DNA of opportunistic, phytopathogenic and symbiotic fungi were detected, which might have been introduced by human activities, transported by birds or wind, and/or represent resident fungi not previously reported from Antarctica. Further long-term studies are required to elucidate how biological colonization in the island may be affected by climatic changes and/or other anthropogenic influences. |
format |
Text |
author |
Rosa, Luiz Henrique da Silva, Thamar Holanda Ogaki, Mayara Baptistucci Pinto, Otávio Henrique Bezerra Stech, Michael Convey, Peter Carvalho-Silva, Micheline Rosa, Carlos Augusto Câmara, Paulo E. A. S. |
author_facet |
Rosa, Luiz Henrique da Silva, Thamar Holanda Ogaki, Mayara Baptistucci Pinto, Otávio Henrique Bezerra Stech, Michael Convey, Peter Carvalho-Silva, Micheline Rosa, Carlos Augusto Câmara, Paulo E. A. S. |
author_sort |
Rosa, Luiz Henrique |
title |
DNA metabarcoding uncovers fungal diversity in soils of protected and non-protected areas on Deception Island, Antarctica |
title_short |
DNA metabarcoding uncovers fungal diversity in soils of protected and non-protected areas on Deception Island, Antarctica |
title_full |
DNA metabarcoding uncovers fungal diversity in soils of protected and non-protected areas on Deception Island, Antarctica |
title_fullStr |
DNA metabarcoding uncovers fungal diversity in soils of protected and non-protected areas on Deception Island, Antarctica |
title_full_unstemmed |
DNA metabarcoding uncovers fungal diversity in soils of protected and non-protected areas on Deception Island, Antarctica |
title_sort |
dna metabarcoding uncovers fungal diversity in soils of protected and non-protected areas on deception island, antarctica |
publisher |
Nature Publishing Group UK |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738542/ http://www.ncbi.nlm.nih.gov/pubmed/33319803 https://doi.org/10.1038/s41598-020-78934-7 |
long_lat |
ENVELOPE(-60.633,-60.633,-62.950,-62.950) |
geographic |
Deception Island South Shetland Islands |
geographic_facet |
Deception Island South Shetland Islands |
genre |
Antarc* Antarctica Deception Island South Shetland Islands |
genre_facet |
Antarc* Antarctica Deception Island South Shetland Islands |
op_source |
Sci Rep |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738542/ http://www.ncbi.nlm.nih.gov/pubmed/33319803 http://dx.doi.org/10.1038/s41598-020-78934-7 |
op_rights |
© The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1038/s41598-020-78934-7 |
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Scientific Reports |
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10 |
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1 |
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1766231908727914496 |